Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular Acinetobacter baumannii
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Cell Culture
2.2. Cell Culture and Infection
2.3. Flow Cytometry
2.4. Fluorescent Microscopy
2.5. RNA Isolation
2.6. Read Mapping and Differential Expression Analysis
2.7. Clustering and Functional Enrichment Analysis
3. Results and Discussion
3.1. Acinetobacter baumannii Can Survive inside Epithelial Cells
3.2. Acinetobacter baumannii Reorganizes the Cell Transcriptome after Intracellular Invasion
3.3. Regulation of Host Genes in Response to A. baumannii Infection
3.4. Gene Expression Changes Are Correlated at the Level of Protein–Protein Interactions
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Crua Asensio, N.; Macho Rendón, J.; Torrent Burgas, M. Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular Acinetobacter baumannii. Microorganisms 2021, 9, 354. https://doi.org/10.3390/microorganisms9020354
Crua Asensio N, Macho Rendón J, Torrent Burgas M. Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular Acinetobacter baumannii. Microorganisms. 2021; 9(2):354. https://doi.org/10.3390/microorganisms9020354
Chicago/Turabian StyleCrua Asensio, Nuria, Javier Macho Rendón, and Marc Torrent Burgas. 2021. "Time-Resolved Transcriptional Profiling of Epithelial Cells Infected by Intracellular Acinetobacter baumannii" Microorganisms 9, no. 2: 354. https://doi.org/10.3390/microorganisms9020354