Isoprene-Degrading Bacteria from Soils Associated with Tropical Economic Crops and Framework Forest Trees
Abstract
:1. Introduction
2. Materials and Methods
2.1. Soil Sampling
2.2. Preparation of Soil Samples for Isoprene Degradation Measurement
2.3. Analysis of Isoprene Degradation by Gas Chromatography-Flame Ionisation Detection (GC-FID)
2.4. Isolation and Identification of Isoprene-Degrading Bacteria
2.5. Microbial Community Analysis and Bioinformatics
2.5.1. Differences in Bacterial Community Composition
2.5.2. Phylogenetic Analysis
2.6. Measurement of Isoprene-Degrading Activity of Bacterial Isolates
3. Results and Discussion
3.1. Isoprene Degradation by Soil Microbes Associated with Economic Crop Species and Framework Forest Tree Species
3.2. Bacterial Community Analysis and Identification of Taxa That Increase in Relative Abundance after Isoprene Enrichment
3.3. Isoprene-Degrading Bacteria Isolated from Soil Samples
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Type of Plant | Common Name | Scientific Name |
---|---|---|
Framework tree | Council tree | Ficus altissima BI. |
Wild oak | Quercus semiserrata Roxb. | |
Wild Himalayan cherry | Prunus cerasoides D. Don | |
Economic crop | Oil palm | Elaeis guineensis Jacq. |
Rubber tree | Hevea brasiliensis Muell. Arg. | |
Sugar cane | Saccharum officinarum L. |
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Uttarotai, T.; McKew, B.A.; Benyahia, F.; Murrell, J.C.; Mhuantong, W.; Wangkarn, S.; Chitov, T.; Bovonsombut, S.; McGenity, T.J. Isoprene-Degrading Bacteria from Soils Associated with Tropical Economic Crops and Framework Forest Trees. Microorganisms 2021, 9, 1024. https://doi.org/10.3390/microorganisms9051024
Uttarotai T, McKew BA, Benyahia F, Murrell JC, Mhuantong W, Wangkarn S, Chitov T, Bovonsombut S, McGenity TJ. Isoprene-Degrading Bacteria from Soils Associated with Tropical Economic Crops and Framework Forest Trees. Microorganisms. 2021; 9(5):1024. https://doi.org/10.3390/microorganisms9051024
Chicago/Turabian StyleUttarotai, Toungporn, Boyd A. McKew, Farid Benyahia, J. Colin Murrell, Wuttichai Mhuantong, Sunanta Wangkarn, Thararat Chitov, Sakunnee Bovonsombut, and Terry J. McGenity. 2021. "Isoprene-Degrading Bacteria from Soils Associated with Tropical Economic Crops and Framework Forest Trees" Microorganisms 9, no. 5: 1024. https://doi.org/10.3390/microorganisms9051024