Detection of KPC, NDM and VIM-Producing Organisms Directly from Rectal Swabs by a Multiplex Lateral Flow Immunoassay
Abstract
:1. Introduction
2. Materials and Methods
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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No | Bacterial Isolate | PCR | Combined Disc Test with PBA and EDTA | NG-Test Carba 5 |
---|---|---|---|---|
1 | (1) K. pneumoniae (2) P. aeuginosa | blaKPC + blaVIM | (1) carbapenemase inhibited by PBA (2) carbapenemase inhibited by EDTA | KPC + VIM |
2 | P. aeruginosa | blaVIM | carbapenemase inhibited by EDTA | VIM |
3 | K. pneumoniae | blaKPC | carbapenemase inhibited by PBA | KPC |
4 | K. pneumoniae | blaKPC + blaVIM | carbapenemase inhibited by PBA + EDTA | KPC + VIM |
5 | (1) K. pneumoniae (2) No VIM producing organism isolated | blaKPC + blaVIM | (1) carbapenemase inhibited by PBA | KPC + VIM |
6 | K. pneumoniae | blaNDM | carbapenemase inhibited by ΕDTA | NDM |
7 | K. pneumoniae | blaKPC | carbapenemase inhibited by PBA | KPC |
8 | K. pneumoniae | blaNDM | carbapenemase inhibited by EDTA | NDM |
9 | (1) K. pneumoniae (2) P. aeruginosa | blaKPC + blaVIM | (1) carbapenemase inhibited by PBA (2) carbapenemase inhibited by EDTA | KPC + VIM |
10 | K. pneumoniae | blaKPC | carbapenemase inhibited by PBA | KPC |
11 | P. aeruginosa | blaVIM | carbapenemase inhibited by EDTA | VIM |
12 | P. aeruginosa | blaVIM | carbapenemase inhibited by EDTA | VIM |
13 | K. pneumoniae | blaKPC | carbapenemase inhibited by PBA | KPC |
14 | K. pneumoniae | blaKPC + blaVIM | carbapenemase inhibited by PBA + EDTA | KPC + VIM |
15 | K. pneumoniae | blaNDM | carbapenemase inhibited by EDTA | NDM |
16 | K. pneumoniae | blaNDM | carbapenemase inhibited by EDTA | NDM |
17 | K. pneumoniae | blaKPC | carbapenemase inhibited by PBA | KPC |
18 | K. pneumoniae | blaKPC | carbapenemase inhibited by PBA | KPC |
19 | K. pneumoniae | blaNDM | carbapenemase inhibited by EDTA | NDM |
20 | K. pneumoniae | blaNDM | carbapenemase inhibited by EDTA | NDM |
Carbapenemase | Broth Incubation Time (h) | TP | FN | Sensitivity | TN | FP | Specificity |
---|---|---|---|---|---|---|---|
KPC | 0 h | 7 | 4 | 63.6% | 19 | 0 | 100% |
1 h | 8 | 3 | 72.7% | 19 | 0 | 100% | |
2 h | 9 | 2 | 81.8% | 19 | 0 | 100% | |
3 h | 11 | 0 | 100% | 19 | 0 | 100% | |
4 h | 11 | 0 | 100% | 19 | 0 | 100% | |
VIM | 0 h | 7 | 1 | 87.5% | 22 | 0 | 100% |
1 h | 8 | 0 | 100% | 22 | 0 | 100% | |
2 h | 8 | 0 | 100% | 22 | 0 | 100% | |
3 h | 8 | 0 | 100% | 22 | 0 | 100% | |
4 h | 8 | 0 | 100% | 22 | 0 | 100% | |
NDM | 0 h | 6 | 0 | 100% | 24 | 0 | 100% |
1 h | 6 | 0 | 100% | 24 | 0 | 100% | |
2 h | 6 | 0 | 100% | 24 | 0 | 100% | |
3 h | 6 | 0 | 100% | 24 | 0 | 100% | |
4 h | 6 | 0 | 100% | 24 | 0 | 100% |
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Vasilakopoulou, A.; Karakosta, P.; Vourli, S.; Kalogeropoulou, E.; Pournaras, S. Detection of KPC, NDM and VIM-Producing Organisms Directly from Rectal Swabs by a Multiplex Lateral Flow Immunoassay. Microorganisms 2021, 9, 942. https://doi.org/10.3390/microorganisms9050942
Vasilakopoulou A, Karakosta P, Vourli S, Kalogeropoulou E, Pournaras S. Detection of KPC, NDM and VIM-Producing Organisms Directly from Rectal Swabs by a Multiplex Lateral Flow Immunoassay. Microorganisms. 2021; 9(5):942. https://doi.org/10.3390/microorganisms9050942
Chicago/Turabian StyleVasilakopoulou, Alexandra, Polyxeni Karakosta, Sophia Vourli, Eleni Kalogeropoulou, and Spyros Pournaras. 2021. "Detection of KPC, NDM and VIM-Producing Organisms Directly from Rectal Swabs by a Multiplex Lateral Flow Immunoassay" Microorganisms 9, no. 5: 942. https://doi.org/10.3390/microorganisms9050942
APA StyleVasilakopoulou, A., Karakosta, P., Vourli, S., Kalogeropoulou, E., & Pournaras, S. (2021). Detection of KPC, NDM and VIM-Producing Organisms Directly from Rectal Swabs by a Multiplex Lateral Flow Immunoassay. Microorganisms, 9(5), 942. https://doi.org/10.3390/microorganisms9050942