Effect of Fibroblast Growth Factor 10 and an Interacting Non-Coding RNA on Secondary Hair Follicle Dermal Papilla Cells in Cashmere Goats’ Follicle Development Assessed by Whole-Transcriptome Sequencing Technology
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Preparation
2.2. Library Preparation and Illumina Hiseq xten/NovaSeq6000 Sequencing
2.3. Sequencing Data Quality Control
2.4. Sequence Alignment
2.5. Transcript Assembly and Functional Annotation
2.6. Identification and Prediction of ncRNAs
2.7. Differential Expression Analysis
2.8. ncRNA Target Gene Prediction
2.9. Functional Enrichment Analysis
2.10. Construction of a ceRNA Network
2.11. Construction of Vectors
2.12. Morphological Observation
2.13. Target Verification
2.14. Assessment of Cell Proliferation
2.15. Detection of Apoptosis
2.16. Cell Cycle Analysis
2.17. Reverse-Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) Validation
2.18. Western Blotting
3. Results
3.1. HF Morphogenesis
3.2. RNA Sequencing and cDNA Library Construction
3.3. Expression Analysis and Functional Annotation of mRNAs
3.4. Expression Analysis and Functional Annotation of circRNAs
3.5. Expression Analysis and Functional Annotation of miRNAs
3.6. IPath Metabolic Pathway Analysis
3.7. Construction of ceRNA Network
3.8. RT-qPCR Validation
3.9. Verification of the Targeting Relationship between FGF10 and ncRNA
3.10. miR-184 Inhibits the Proliferation of SHF-DPCs by Targeting FGF10
3.11. miR-184 Inhibits Apoptosis in SHF-DPCs by Targeting FGF10
3.12. miR-184 Affects Cell Cycle Progression in SHF-DPCs by Targeting FGF10
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Gao, Y.; Song, W.; Hao, F.; Duo, L.; Zhe, X.; Gao, C.; Guo, X.; Liu, D. Effect of Fibroblast Growth Factor 10 and an Interacting Non-Coding RNA on Secondary Hair Follicle Dermal Papilla Cells in Cashmere Goats’ Follicle Development Assessed by Whole-Transcriptome Sequencing Technology. Animals 2023, 13, 2234. https://doi.org/10.3390/ani13132234
Gao Y, Song W, Hao F, Duo L, Zhe X, Gao C, Guo X, Liu D. Effect of Fibroblast Growth Factor 10 and an Interacting Non-Coding RNA on Secondary Hair Follicle Dermal Papilla Cells in Cashmere Goats’ Follicle Development Assessed by Whole-Transcriptome Sequencing Technology. Animals. 2023; 13(13):2234. https://doi.org/10.3390/ani13132234
Chicago/Turabian StyleGao, Yuan, Weiguo Song, Fei Hao, Lei Duo, Xiaoshu Zhe, Chunyan Gao, Xudong Guo, and Dongjun Liu. 2023. "Effect of Fibroblast Growth Factor 10 and an Interacting Non-Coding RNA on Secondary Hair Follicle Dermal Papilla Cells in Cashmere Goats’ Follicle Development Assessed by Whole-Transcriptome Sequencing Technology" Animals 13, no. 13: 2234. https://doi.org/10.3390/ani13132234