Exploration of Synergistic Regulation Mechanisms of Cerebral Ganglion and Muscle in Eriocheir sinensis Activated in Response to Alkalinity Stress
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Animals and Alkalinity Stress
2.2. Proteomics Analysis of the Cerebral Ganglion of E. sinensis
2.2.1. Sample Collection
2.2.2. Proteomics Analysis
Protein Extraction, Separation, Enzymolysis, and Desalination
Liquid Chromatography–Tandem Mass Spectrometry/Mass Spectrometry (LC-MS/MS) Analysis and Database Query
Gene Ontology (GO) Annotation on Proteomics Analysis
Enrichment Analysis on Differentially Expressed Proteins (DEPs)
2.3. Metabolomic Analysis of the Muscle of E. sinensis
2.3.1. Sample Collection
2.3.2. Metabolomics Analysis
Extraction of Metabolites from the Muscle of E. sinensis and Ultra Performance Liquid Chromatography/Mass Chromatography (UPLC-MS) Analysis
Principal Component Analysis (PCA) and Partial Least Squares Discrimination Analysis (PLS-DA) on Differential Expressed Metabolites (DEMs)
KEGG Enrichment Analysis on DEMs
2.4. Combined Proteomic and Metabolomic Analyses on Cerebral Ganglion and Muscle
3. Results
3.1. Proteomics Analysis
3.1.1. Basic Statistics and Annotation of Cerebral Ganglion Proteome
Peptide Length and Molecular Weight Distribution
GO Annotation of Proteome
3.1.2. Enrichment Analysis of the Top 20 KEGG Pathways
3.2. Metabolomics Analysis
3.2.1. Multivariate Statistics of Differentially Expressed Metabolites
PCA Analysis of Differential Metabolites
PLS-DA Analysis of Differential Metabolites
3.2.2. Enrichment Analysis on Top 20 KEGG Pathways
3.3. Combined Proteomics and Metabolomics Analyses of Cerebral Ganglion and Muscle of E. sinensis under Acute Alkalinity Stress
3.3.1. Co-Enrichment Pathways of Cerebral Ganglion Proteome and Muscle Metabolome
3.3.2. Synergistic Regulation Mechanisms of Cerebral Ganglion and Muscle of E. sinensis under Alkalinity Stress
4. Discussion
4.1. Synergistic Regulation of Amino Acid Metabolism in Cerebral Ganglion and Muscle of E. sinensis under Alkalinity Stress
4.2. Synergistic Regulation of Energy Metabolism under Alkalinity Stress
4.3. Synergistic Regulation of Signal Transduction and Immunoregulation under Alkalinity Stress
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Classification | Log2FC | p-Value | Regulation |
---|---|---|---|---|
4-aminobutyrate aminotransferase | DEP | inf | 2.94 × 10−6 | up |
alpha-Ketoglutaric acid | DEM | 1.81 | 4.90 × 10−3 | up |
Citric acid | DEM | 0.73 | 4.28 × 10−2 | up |
Adenylosuccinic acid | DEM | 0.99 | 2.95 × 10−2 | up |
L-Glutamic acid | DEM | 0.71 | 3.63 × 10−2 | up |
Prostaglandin H2 | DEM | 1.51 | 3.79 × 10−2 | up |
Prostaglandin B2 | DEM | 1.67 | 1.29 × 10−2 | up |
Leukotriene C4 | DEM | 1.197 | 4.09 × 10−2 | up |
Lipoxin B4 | DEM | 1.52 | 1.50 × 10−3 | up |
ABAT | DEP | inf | 2.94 × 10−6 | up |
Aldose reductase | DEP | 0.2 | 4.00 × 10−4 | up |
Calcium-transporting ATPase | DEP | −0.39 | 2.87 × 10−2 | down |
Ryanodine receptor 5 | DEP | −3.07 | 1.50 × 10−2 | down |
Ryanodine receptor 9 | DEP | 0.12 | 2.77 × 10−3 | up |
Ryanodine receptor 2 | DEP | 0.35 | 1.12 × 10−3 | up |
Ryanodine receptor 3 | DEP | 0.21 | 3.75 × 10−4 | up |
Reduced nicotinamide adenine dinucleotide | DEM | −5.25 | 5.60 × 10−3 | down |
Uridine | DEM | −0.54 | 1.04 × 10−2 | down |
Cytidine-5′-monophosphate | −4.81 | 8.90 × 10−3 | down | |
17alpha-Hydroxyprogesterone | DEM | 0.32 | 3.02 × 10−3 | up |
Serine hydroxymethyltransferase | DEP | 0.63 | 5.72 × 10−4 | up |
Acetyl-coA acetyltransferase | DEP | 0.98 | 1.07 × 10−4 | up |
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Wang, M.; Zhou, J.; Ge, J.; Tang, Y.; Xu, G. Exploration of Synergistic Regulation Mechanisms of Cerebral Ganglion and Muscle in Eriocheir sinensis Activated in Response to Alkalinity Stress. Animals 2024, 14, 2374. https://doi.org/10.3390/ani14162374
Wang M, Zhou J, Ge J, Tang Y, Xu G. Exploration of Synergistic Regulation Mechanisms of Cerebral Ganglion and Muscle in Eriocheir sinensis Activated in Response to Alkalinity Stress. Animals. 2024; 14(16):2374. https://doi.org/10.3390/ani14162374
Chicago/Turabian StyleWang, Meiyao, Jun Zhou, Jiachun Ge, Yongkai Tang, and Gangchun Xu. 2024. "Exploration of Synergistic Regulation Mechanisms of Cerebral Ganglion and Muscle in Eriocheir sinensis Activated in Response to Alkalinity Stress" Animals 14, no. 16: 2374. https://doi.org/10.3390/ani14162374