LEP Gene Promotes Milk Fat Synthesis via the JAK2-STAT3 and mTOR Signaling Pathways in Buffalo Mammary Epithelial Cells
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Gene Cloning and Identification
2.3. Bioinformatics Analysis
2.4. Overexpression Vector Construction
2.5. Cell Culture and Transduction
2.6. Gene Expression Detection and Analysis
2.7. TG Test in BuMECs
2.8. Detection and Analysis of LEP Polymorphisms
2.9. Data Statistics
3. Results
3.1. Cloning and Identification of Buffalo LEP
3.2. Transcriptional Region Structure of LEP
3.3. Identity of Amino Acid Sequences and Phylogenetic Tree of LEP
3.4. Structures and Physicochemical Characteristics of LEP Protein
3.5. Signal Peptide and Transmembrane Region
3.6. Biological Process, Molecular Function, and Functional Modification Site
3.7. Differential Expression of LEP
3.8. Overexpression of LEP_X1 Upregulates Genes Related to Milk Fat Synthesis in BuMECs and Increases TG Concentration
3.9. Overexpression of LEP_X2 Upregulates the Genes Related to Milk Fat Synthesis in BuMECs and Increases TG Concentration
3.10. Antagonism Interaction between LEP_X1 and LEP_X2
3.11. Population Variation Analysis
3.12. Sequence Differences Among Bovidae Species
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Population | SNP | Genotype | Genotype Frequency (%) | Allele | Allele Frequency (%) | p-Value 1 |
---|---|---|---|---|---|---|
River buffalo | c.148C>T | CC | 1.0000 | C T | 1.0000 0.0000 | − |
CT | 0.0000 | |||||
TT | 0.0000 | |||||
c.435G>A | AA | 0.0417 | A G | 0.1667 0.8333 | 0.53751 | |
AG | 0.2500 | |||||
GG | 0.7083 | |||||
c.450G>A | AA | 0.0417 | A G | 0.2500 0.7500 | 0.65497 | |
AG | 0.4167 | |||||
GG | 0.5416 | |||||
c.558G>A | AA | 0.0417 | A G | 0.1042 0.8958 | 0.06186 | |
AG | 0.1250 | |||||
GG | 0.8333 | |||||
Swamp buffalo | c.148C>T | CC | 0.9756 | C T | 0.9878 0.0122 | 1.00000 |
CT | 0.0244 | |||||
TT | 0.0000 | |||||
c.435G>A | AA | 0.4634 | A G | 0.5610 0.4390 | 0.00008 | |
AG | 0.1951 | |||||
GG | 0.3415 | |||||
c.450G>A | AA | 0.0732 | A G | 0.1707 0.8293 | 0.03474 | |
AG | 0.1951 | |||||
GG | 0.7317 | |||||
c.558G>A | AA | 0.0976 | A G | 0.2195 0.7805 | 0.05150 | |
AG | 0.2439 | |||||
GG | 0.6585 |
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Gao, R.; Zhu, Q.; Huang, L.; Fan, X.; Teng, X.; Miao, Y. LEP Gene Promotes Milk Fat Synthesis via the JAK2-STAT3 and mTOR Signaling Pathways in Buffalo Mammary Epithelial Cells. Animals 2024, 14, 2446. https://doi.org/10.3390/ani14162446
Gao R, Zhu Q, Huang L, Fan X, Teng X, Miao Y. LEP Gene Promotes Milk Fat Synthesis via the JAK2-STAT3 and mTOR Signaling Pathways in Buffalo Mammary Epithelial Cells. Animals. 2024; 14(16):2446. https://doi.org/10.3390/ani14162446
Chicago/Turabian StyleGao, Ruixia, Qunyao Zhu, Lige Huang, Xinyang Fan, Xiaohong Teng, and Yongwang Miao. 2024. "LEP Gene Promotes Milk Fat Synthesis via the JAK2-STAT3 and mTOR Signaling Pathways in Buffalo Mammary Epithelial Cells" Animals 14, no. 16: 2446. https://doi.org/10.3390/ani14162446