Ningxiang Pig-Derived Microbiota Affects the Growth Performance, Gut Microbiota, and Serum Metabolome of Nursery Pigs
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Design
2.2. Sample Collection
2.3. Measurement of Apparent Total Tract Digestibility
2.4. Measurement of Antioxidant Indicators
2.5. DNA Extraction, 16S rRNA Sequencing of Fecal Microbiota and Data Analysis
2.6. Untargeted Metabolomics Study of Serum
2.7. Statistical Analysis
3. Results
3.1. Growth Performance, Apparent Digestibility, and Serum Antioxidant Activity
3.2. Ningxiang Pig-Derived Microbiota Shaped the Gut Microbiome in DLY Pigs
3.3. Ningxiang Pig-Derived Microbiota Altered the Serum Metabolism of DLY Pigs
3.4. Correlation between Serum Metabolites and Fecal Microbiota, Fecal Microbiota, and Digestibility
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Items | CON 1 | FMT 2 | p-Value |
---|---|---|---|
Initial weight (kg) | 19.79 ± 0.31 | 19.58 ± 0.30 | 0.620 |
Final weight (kg) | 35.41 ± 0.89 | 38.60 ± 1.31 | 0.055 |
ADG (g/d) | 558.10 ± 33.87 | 679.52 ± 47.15 | 0.046 |
Items | CON 1 | FMT 2 | p-Value |
---|---|---|---|
DM | 86.8 ± 0.09 | 86.08 ± 0.27 | 0.039 |
GE | 87.96 ± 0.06 | 88.65 ± 0.10 | <0.001 |
EE | 86.31 ± 0.58 | 84.6 ± 0.84 | 0.122 |
CP | 91.33 ± 0.22 | 90.93 ± 0.15 | 0.178 |
CF | 42.08 ± 0.83 | 47.76 ± 1.62 | 0.011 |
ASH | 64.06 ± 0.53 | 67.42 ± 0.40 | <0.001 |
Ca | 50.59 ± 0.87 | 55.12 ± 1.56 | 0.026 |
P | 48.95 ± 0.75 | 51.77 ± 1.44 | 0.102 |
Items | CON 1 | FMT 2 | p-Value |
---|---|---|---|
CAT (U/mL) | 20.00 ± 2.86 | 30.79 ± 3.66 | 0.039 |
TAOC (U/mL) | 2.38 ± 0.48 | 3.58 ± 1.14 | 0.351 |
TSOD (U/mL) | 56.43 ± 5.05 | 70.97 ± 3.09 | 0.034 |
MDA (nmol/mL) | 15.05 ± 3.31 | 5.21 ± 0.63 | 0.027 |
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Li, H.; Han, L.; Zhou, F.; Wu, Z.; Zhang, L.; Xie, R.; Jiang, F.; Tian, Q.; Huang, X. Ningxiang Pig-Derived Microbiota Affects the Growth Performance, Gut Microbiota, and Serum Metabolome of Nursery Pigs. Animals 2024, 14, 2450. https://doi.org/10.3390/ani14172450
Li H, Han L, Zhou F, Wu Z, Zhang L, Xie R, Jiang F, Tian Q, Huang X. Ningxiang Pig-Derived Microbiota Affects the Growth Performance, Gut Microbiota, and Serum Metabolome of Nursery Pigs. Animals. 2024; 14(17):2450. https://doi.org/10.3390/ani14172450
Chicago/Turabian StyleLi, Hongkun, Li Han, Feng Zhou, Zichen Wu, Longlin Zhang, Renjie Xie, Feng Jiang, Qiyu Tian, and Xingguo Huang. 2024. "Ningxiang Pig-Derived Microbiota Affects the Growth Performance, Gut Microbiota, and Serum Metabolome of Nursery Pigs" Animals 14, no. 17: 2450. https://doi.org/10.3390/ani14172450