Heat Stress Induces Alterations in Gene Expression of Actin Cytoskeleton and Filament of Cellular Components Causing Gut Disruption in Growing–Finishing Pigs
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Treatments
2.2. Sample Collection
2.3. Transcriptomic Analysis
2.4. Bioinformatic Analysis
2.5. RNA Extraction and Quantitative Real-Time PCR
2.6. Statistical Analysis
3. Results
3.1. Analysis of DEGs
3.2. Clustering and Functional Enrichment Analysis
3.3. Protein-Protein Interaction (PPI) Network Analysis
3.4. Key Candidate Genes Involving in Actin Cytoskeleton and Filament Formation Pathway
3.5. Quantitative Real-Time PCR Validation of Gene Expression
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer Sequence (5′-3′) | Product Size (bp) | GenBank No. |
---|---|---|---|
ZO1 | F GGGGCAATCTCAACTCCTGT R GGTTGTCCAACTTGGGCAT | 137 | XM 021098896.1 |
CLDN1 | F TTTCCTCAATACAGGAGGGAAGC R CCCTCTCCCCACATTCGAG | 74 | NM_001244539.1 |
CLDN3 | F GCCAAGATCCTCTACTCCGC R GAGAGCTGCCTAGCATCTGG | 197 | NM_001160075.1 |
CLDN4 | F CTCTCGGACACCTTCCCAAG R GCAGTGGGGAAGGTCAAAGG | 192 | XM_005661969.2 |
OCLN | F TCTCAGCCAGCGTATTCTTTC R GCACATCACGATAACGAGCAT | 111 | XM 005672525.3 |
ACTG2 | F CCTGGCATTGCCGACAGGAT R GGCCAGGATAGAGCCTCCGA | 123 | XM_021087371.1 |
DES | F TTCCGAGCGGATGTGGATGC R CTGAAGCTGGGCCTGCAGTT | 135 | NM_001001535.1 |
PYGM | F GTGGAGATGGCGGAAGAGGC R TCGATGACGTGCCGAAGCTC | 140 | XM_003122588.5 |
MYL9 | F CACCAAGAAGCGGCCACAGA R TGCCCTCCAGGTACTCGTCC | 188 | NM_001244472.1 |
MYLK | F ATCAGGGAGTCCCGCCACTT R CTGCTGTGCAGGTGGCTTCT | 142 | XM_001929078.6 |
PPP1R14A | F TGAGCAAGCTGCAGTCTCCG R CGGTACAGCTCCTCCAAGCG | 163 | NM_214337.1 |
TPM1 | F TGAGCTGGTGTCGCTGCAAA R ATCGGCTTCAGCATCGGTGG | 130 | NM_001097483.2 |
TPM2 | F GATGTGGCCTCCCTGAACCG R TTCTCGGCCTCCTCCAGCTT | 103 | NM_001129947.1 |
CNN1 | F GCAGGAGCAGGAGCTTCGAG R CTGGTGCCAGTTCTGGGTGG | 166 | NM_213878.1 |
PDLIM3 | F GCTGGCGGCACTCAGAAGAT R TTCGCAGTACAGCTCCCCCT | 174 | NM_001001637.1 |
PDLIM7 | F AGCAGAATGGACAGCCGCTC R GAGGATGCGGAAGGAACGGG | 122 | XM_021084391.1 |
PCP4 | F GCTGGGGCCACCAATGGAAA R TGAGACTGAATGGCCACCGC | 128 | XM_021071001.1 |
PCK1 | F TGCTCCCGGAACCTCTGTGA R GGTCGATGATGGGGCACTGG | 107 | NM_001123158.1 |
PCK2 | F TCTTTCGGCAGCGGCTATGG R ACATAGCGCTTCTTCCCCGC | 152 | NM_001161753.1 |
GAPDH | F CCACGGTCCATGCCATCACT R GCCTGCTTCACCACCTTCTTG | 268 | XM_021091114.1 |
Gene ID | Gene Name | Universal Gene Name |
---|---|---|
100736682 | ZO1 | zonula occludens-1 |
100625166 | CLDN1 | claudin 1 |
431781 | CLDN3 | claudin 3 |
733578 | CLDN4 | claudin 4 |
397236 | OCLN | occludin |
100520667 | ACTG2 | actin gamma 2, smooth muscle |
396725 | DES | desmin |
733659 | PYGM | glycogen phosphorylase |
100157760 | MYL9 | myosin light chain 9 |
396848 | MYLK | myosin light chain kinase |
397610 | PPP1R14A | protein phosphatase 1 regulatory inhibitor subunit 14A |
100037999 | TPM1 | tropomyosin 1 |
396693 | TPM2 | tropomyosin 2 |
396911 | CNN1 | calponin 1 |
414421 | PDLIM3 | PDZ and LIM domain 3 |
100624749 | PDLIM7 | PDZ and LIM domain 7 |
110256491 | PCP4 | Purkinje cell protein 4 |
100144531 | PCK1 | phosphoenolpyruvate carboxykinase 1 |
403165 | PCK2 | phosphoenolpyruvate carboxykinase 2 |
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Choi, Y.; Park, H.; Kim, J.; Lee, H.; Kim, M. Heat Stress Induces Alterations in Gene Expression of Actin Cytoskeleton and Filament of Cellular Components Causing Gut Disruption in Growing–Finishing Pigs. Animals 2024, 14, 2476. https://doi.org/10.3390/ani14172476
Choi Y, Park H, Kim J, Lee H, Kim M. Heat Stress Induces Alterations in Gene Expression of Actin Cytoskeleton and Filament of Cellular Components Causing Gut Disruption in Growing–Finishing Pigs. Animals. 2024; 14(17):2476. https://doi.org/10.3390/ani14172476
Chicago/Turabian StyleChoi, Yohan, Hyunju Park, Joeun Kim, Hyunseo Lee, and Minju Kim. 2024. "Heat Stress Induces Alterations in Gene Expression of Actin Cytoskeleton and Filament of Cellular Components Causing Gut Disruption in Growing–Finishing Pigs" Animals 14, no. 17: 2476. https://doi.org/10.3390/ani14172476