Protective Effects of Tea Tree Oil on Inflammatory Injury of Porcine Intestinal Epithelial Cells Induced by Lipopolysaccharide In Vitro
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Line Culture
2.2. Experimental Design
2.3. Cell Viability
2.4. Cytokine Secretion
2.5. Cell Apoptosis
2.6. Gene Expression
2.7. Statistical Analysis
3. Results
3.1. Cell Viability after LPS Stimulation
3.2. Cytokine Secretion after LPS Stimulation
3.3. Gene Expression of Cytokines after LPS Stimulation
3.4. Viability of IPI-2I Cells after Tea Tree Oil Treatment
3.5. Cell Apoptosis
3.6. Cytokine Secretion and Gene Expression
3.7. Gene Expression of NF-κB and TLR4
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer Sequence (5′-3′) | Fragment, bp |
---|---|---|
GAPDH | 5′ GTCGGAGTGAACGGATTTGGC 3′ 5′ GGAGGTCAATGAAGGGGTCA 3′ | 106 |
IL-1β | 5′ GTGGCAGGACCTACACTCTTC 3′ 5′ TTCCTTCAGAATGCCGTCCTC 3′ | 115 |
IL10 | 5′ GTGGCAGCCAGCATTAAGTC 3′ 5′ AACTCTTCACTGGGCCGAAG 3′ | 103 |
IFN-γ | 5′ GGCCATTCAAAGGAGCATGGA 3′ 5′ TCACTGATGGCTTTGCGCT 3′ | 144 |
TNF-α | 5′ GCCCTTCCACCAACGTTTTC 3′ 5′ CAAGGGCTCTTGATGGCAGA 3′ | 97 |
NF-κB p65 | 5′ AGATCTTCCTGCTGTGCGAC 3′ 5′ GTCGGCTTGTGAAAAGGAGC 3′ | 98 |
TLR4 | 5′ GTGGCCTCCAAGGAACAAGA 3′ 5′ CTGGTGTTCACACGCACAAG 3′ | 96 |
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Dong, L.; Yuan, Q.; Qiu, G.; Zhang, Y.; Wang, H.; Yu, L. Protective Effects of Tea Tree Oil on Inflammatory Injury of Porcine Intestinal Epithelial Cells Induced by Lipopolysaccharide In Vitro. Animals 2024, 14, 2577. https://doi.org/10.3390/ani14172577
Dong L, Yuan Q, Qiu G, Zhang Y, Wang H, Yu L. Protective Effects of Tea Tree Oil on Inflammatory Injury of Porcine Intestinal Epithelial Cells Induced by Lipopolysaccharide In Vitro. Animals. 2024; 14(17):2577. https://doi.org/10.3390/ani14172577
Chicago/Turabian StyleDong, Li, Qingqing Yuan, Guangzhi Qiu, Yongsheng Zhang, Hongrong Wang, and Lihuai Yu. 2024. "Protective Effects of Tea Tree Oil on Inflammatory Injury of Porcine Intestinal Epithelial Cells Induced by Lipopolysaccharide In Vitro" Animals 14, no. 17: 2577. https://doi.org/10.3390/ani14172577