POLB Regulates Proliferation and Apoptosis of Bovine Primary Myocytes
by
,
Geyang Zhang
1,2,†,
Jiamei Wang
3,†,
Yulong Li
1,
Zijing Zhang
1,
Xiangnan Wang
1,
Fuying Chen
1,
Qiaoting Shi
1,
Yongzhen Huang
3,
Eryao Wang
1,* and
Shijie Lyu
1,4,* 1
Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
2
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China
3
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
4
The Shennong Laboratory, Zhengzhou 450002, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to the work.
Animals 2024, 14(9), 1323; https://doi.org/10.3390/ani14091323
Submission received: 6 February 2024
/
Revised: 30 March 2024
/
Accepted: 22 April 2024
/
Published: 28 April 2024
(This article belongs to the Collection Advances in Cattle Breeding, Genetics and Genomics)
Simple Summary
This study focused on the DNA polymerase β (POLB) gene, which belongs to the DNA polymerase X family involved in DNA replication, repair, recombination, and cell cycle regulation. POLB was identified as a muscle development-related gene through gene screening and gene expression analysis methods. Further validation in bovine primary myocytes showed that overexpression of POLB promoted apoptosis, while gene knockdown had no significant effect. Analysis of related genes revealed that POLB overexpression affected the expression of the CASP9 gene, which is involved in the apoptotic pathway.
Abstract
DNA polymerase β (DNA polymerase beta (POLB)) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation. In this study, the muscle development-related gene POLB was screened by selection signature and RNA-seq analysis and then validated for the proliferation and apoptosis of bovine primary myocytes. It was also found that overexpression of the POLB gene had a pro-apoptosis effect, but interfering with the expression of the gene had no significant effect on cells. Then, the analysis of related apoptotic genes revealed that POLB overexpression affected CASP9 gene expression.
