Recent Development of Morphology Controlled Conducting Polymer Nanomaterial-Based Biosensor
Round 1
Reviewer 1 Report
The authors present a nice review of morphology of conductive polymers. The paper is divided in three different macro areas PPy, PANI and PEDOT and, further, in the dimensions of the device 0,1,2,3D. This result in a fast and easy-to-get layout useful for the reader. I like the manuscript and I think this could recieve many citation as the topic is innovative and there are no many summary like this in literature.
The only main concerning I have is about the self citations of the author, I was able to count like 16, and even if there are not inappropriate, I'd like to pass you the message to take care of these and if possible reduce in a manner that not more than 10% of the total citations regard you.
The rest of my comments are mainly typos or small corrections:
line 9: please indicate two email if you indicate 2 stars
line 21: add morphology related keyword
line 75: I'd like to have a citation regarding this affirmation of aqu. and non aqueous enviroments.
line 78: e.g is more appropriate in this case instead of i.e.
table 1: check all the acronyms you used and declare them, FET is not explained.
table 1bis: repeat LOD also in the caption, not only the resolution of the acronym (this apply also to all the following Tables)
line 227: the dash in a list is hasrd to get, prefer something like: "nanometric: rods, spheres...etc"
line 258: resolve PEG acronym
figure 5: please, choose a better resolution for this image
line 291: FET acronym, also if in the caption of Table 1 you repeat it.
figure 7: please, choose a better resolution for this image
part 4.3: you could expand more the topic, I think here you passed to fast and you cite too few results present in literature; the following work is, as example, nice to include : 10.1016/j.snb.2019.127539.
Author Response
Reviewer 1.
COMMENT
The authors present a nice review of morphology of conductive polymers. The paper is divided in three different macro areas PPy, PANI and PEDOT and, further, in the dimensions of the device 0,1,2,3D. This result in a fast and easy-to-get layout useful for the reader. I like the manuscript and I think this could receive many citation as the topic is innovative and there are no many summary like this in literature.
REPONSE:
We highly appreciate your positive comments on our manuscript.
COMMENT 1) The only main concerning I have is about the self citations of the author, I was able to count like 16, and even if there are not inappropriate, I'd like to pass you the message to take care of these and if possible reduce in a manner that not more than 10% of the total citations regard you.
REPONSE:
Thanks for your comment about self-citation. As you said, we reduced number of the self-citation down to 7 in the revised reference part.
COMMENT 2) line 9: please indicate two email if you indicate 2 stars
REPONSE:
Thanks for indicating error about authorship of the manuscript. We corrected correspondence just on the Prof. Lee.
COMMENT 3) line 21: add morphology related keyword
REPONSE:
We added “morphology control” as a keyword.
COMMENT 4) line 75: I'd like to have a citation regarding this affirmation of aqu. and non-aqueous environments.
REPONSE:
Thanks for your comment about reference about aqu. and non-aqueous environments in polymerization. As you commented, we added manuscripts about illustration of the aqu. and non-aqueous environments.[R1,R2]
[R1] Langmuir 2002, 18, 5613-5618.
[R2] Chem. Commun. 2003, 720-721.
Revised parts in the manuscript
(1) [R1] and [R2] were added as “reference 69 and 70” in the revised manuscript.
COMMENT 5) line 78: e.g is more appropriate in this case instead of i.e.
REPONSE:
We changed “i.e.” to “e.g.” in the revised manuscript.
COMMENT 6) table 1: check all the acronyms you used and declare them, FET is not explained.
REPONSE:
We checked all acronyms and added illustration of FET in the revised manuscript.
COMMENT 7) table 1bis: repeat LOD also in the caption, not only the resolution of the acronym (this apply also to all the following Tables)
REPONSE:
As you mentioned, we added illustration of LOD in the all tables.
COMMENT 8) line 227: the dash in a list is hard to get, prefer something like: "nanometric: rods, spheres...etc"
REPONSE:
As you said, we corrected the sentence of line 227.
Revised parts in the manuscript
(1) The sentence “These traits are strongly influenced by the shapes of the materials, namely, whether they are nanofibers, -wires, -rods, -tubes, or -particles [94].” was changed to “These traits are strongly influenced by the shapes of the materials, whether they are nanofibers, nanowires, nanorods, nanotubes, or nanoparticles [96].” in the revised manuscript.
COMMENT 9) line 258: resolve PEG acronym
REPONSE:
We added illustration of PEG as polyethylene glycol in the revised manuscript.
COMMENT 10) figure 5: please, choose a better resolution for this image
REPONSE:
We changed figure 5 using higher resolution version.
COMMENT 11) line 291: FET acronym, also if in the caption of Table 1 you repeat it.
REPONSE:
We added illustration of FET such as field effect transistor” in the revised manuscript.
COMMENT 12) figure 7: please, choose a better resolution for this image
REPONSE:
We changed figure 7 by higher resolution version.
COMMENT 13) part 4.3: you could expand more the topic, I think here you passed to fast and you cite too few results present in literature; the following work is, as example, nice to include : 10.1016/j.snb.2019.127539
REPONSE:
We added more explanation about 2D PEDOT structures.
Revised parts in the manuscript
(1) The paragraph “Moreover, Scotto et al. fabricated layer-by-layer PEDOT/graphite nanosheet-based film using simple spin-coating method [129]. In detail, EDOT and graphite composite solution was deposited by spin-coating process and then exposed oxidant (Fe(III) tosylate) with high temperature. Then, glucose oxidase (GOx) and osmium-modified polyallylamine (OsPA) were coated layer-by-layer on the films surface to detect glucose. In the sensing performance, incorporated graphite nanosheet in the PEDOT structure not only improve the voltammetric response of the resulting all-polymer electrodes but also produces a better integration of the electrochemically active supramolecular assembly allowing the effective glucose sensing. In addition, Wang et al. suggested low fouling electrochemical biosensor using zwitterionic polypeptide doted PEDOT film by simple electrodeposition process to detect breast cancer biomarker (BRCA1) detection [130]. Especially, the polypeptide in the film structure acted as a bifunctional material that not only inhibits the nonspecific adsorption of proteins but also allows for the usage of its carboxylic to fix DNA probes (terminated with amino group).” were added in the revised 4.3 part.
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The revised parts were highlighted by yellow background color. Thank you for your critical and valuable comments again.
Sincerely,
Prof. Junseop Lee
Author Response File: 
Author Response.doc
Reviewer 2 Report
The manuscript is interesting and valuable, even if it could be extended in some parts (e.g. polyaniline micro- and nanofibril based sensors).
The conclusions are "flat" and need to be improved.
Author Response
Reviewer 2.
COMMENT
The manuscript is interesting and valuable, even if it could be extended in some parts (e.g. polyaniline micro- and nanofibril based sensors).
The conclusions are "flat" and need to be improved.
REPONSE:
We highly appreciate your positive comments on our manuscript. As you mentioned, we rewrite conclusion part in the revised manuscript.
Revised parts in the manuscript
(1) The conclusions were changed to “Various conducting polymers are of interest for biosensor applications due to their excellent electrical, chemical, and physical properties. Researchers have utilized these properties to actively optimize the performance of these biosensors by controlling the structure of the conducting polymer in nanoscale such as 0D, 1D, 2D and 3D. In the case of the 0D structure, most researches were conducted to generate uniformly sized conductive polymer nanoparticles using template-based methods (microemulsion and hard template) and then applied to the biosensor electrodes by attaching inorganic components or bio-receptors to the surface. In the case of 1D structure, the tube or fiber structure-based textiles were manufactured using methods such as electrospinning, electrodeposition, and hard template and applied them as a flexible biosensor electrode by attaching various receptors. Using simple electrodeposition, spin coating, and screen printing methods, the 2D structure manufactured a flexible, large-area film and used it as a conductive polymer film-based sensor electrode. Finally, in order to expand the active surface area of the nanomaterials to the analyte, various manufacturing methods were modified to generate the 3D conducting polymer nanostructure, and applied as a highly reactive biosensor electrode material. Insights gained from this field will promote the development of flexible, wearable sensors that could have far reaching consequences in the healthcare industry.” in the revised manuscript.
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The revised parts were highlighted by yellow background color. Thank you for your critical and valuable comments again.
Sincerely,
Prof. Junseop Lee
Author Response File: Author Response.doc
Reviewer 3 Report
Dear Authors,
The proposed paper has been review with reviewer's comments and suggestions as attached file. The paper should been revised in minor.
Yours sincerely,
from Reviewer
Reviewer’s comments:
The paper described the related dimensional (0, 1, 2, and 3D) hetero-nanostructures of conducting polymers has revealed the polymer nanomaterial-based biosensor on morphology controlling the properties of speed of charge transfer processes, working temperature, sensitivity and cycle stability by means of expounding the referred research papers. However, there are some suggestions and comments proposed as below for this review paper.
- Insufficient writing formats and styles should be revised such as the repeat typing on line 19 of page one ( …..the the speed of charge transfer processes……..). The writing formats and styles of manuscript for the proposed paper should been rechecked up.
- The sensing structure, sensing figure of merit, and research results of referred research papers on various sensing types and structures, including the speed of charge transfer processes, working temperature, sensitivity and cycle stability should been clearly compared with each other using a table.
- In section 5, the description of conductive polymer chains in the detection of small RNA should construct a obvious table to explore the receptor, analyte, mechanism , and LOD including various sensing structure .
Comments for author File: 
Comments.pdf
Author Response
Reviewer 3.
COMMENT
The paper described the related dimensional (0, 1, 2, and 3D) hetero-nanostructures of conducting polymers has revealed the polymer nanomaterial-based biosensor on morphology controlling the properties of speed of charge transfer processes, working temperature, sensitivity and cycle stability by means of expounding the referred research papers. However, there are some suggestions and comments proposed as below for this review paper.
REPONSE:
We highly appreciate your positive comments on our manuscript.
COMMENT 1) Insufficient writing formats and styles should be revised such as the repeat typing on line 19 of page one ( …..the the speed of charge transfer processes……..). The writing formats and styles of manuscript for the proposed paper should been rechecked up.
REPONSE:
We modified errors (including line 19) in the manuscript.
COMMENT 2) The sensing structure, sensing figure of merit, and research results of referred research papers on various sensing types and structures, including the speed of charge transfer processes, working temperature, sensitivity and cycle stability should been clearly compared with each other using a table.
REPONSE:
We added index about Response time, working temperature, linearity range, cycle stability in the revised table of the manuscript.
COMMENT 3) In section 5, the description of conductive polymer chains in the detection of small RNA should construct an obvious table to explore the receptor, analyte, mechanism , and LOD including various sensing structure .
REPONSE:
As you mentioned, we added table 4 in the part 5 of the revised version.
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The revised parts were highlighted by yellow background color. Thank you for your critical and valuable comments again.
Sincerely,
Prof. Junseop Lee
Author Response File: Author Response.doc
