Evaluation of Two Commonly Used Field Tests to Assess Varroa destructor Infestation on Honey Bee (Apis mellifera) Colonies
Abstract
:Featured Application
Abstract
1. Introduction
2. Materials and Methods
2.1. Location and Timing
2.2. Colony Measurements and Sample Collection
2.3. Powdered Sugar Roll
2.4. Natural Mite Fall
2.5. Test Protocols
- Protocol 1. Dispersal mite infestation assessment
- Protocol 2. Total mite infestation assessment
- 10-day natural mite fall and dispersal varroa population (protocol 1) or total varroa population (protocol 2)
- mites found with the powdered sugar roll method and dispersal varroa population (protocol 1) or total varroa population (protocol 2)
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Method | Reliability | Sample Size | Sensitivity | References |
---|---|---|---|---|
Soapy wash | 90% when bees are frozen and centrifuged at 6342 rpm | Approx. 100 bees | More than 3 mites per 100 bees, when the infestation level is lower than 3%, efficiency is 85% | [2] |
Equally accurate as powdered sugar test (93%) | 250 frozen bees | [3] | ||
Positive correlations between relative number of infested honey bees detected by powdered sugar shake and washing bees with alcohol plus soapy water (r = 0.90 and r = 0.62) | 300–400 bees | [4] | ||
Hand shaking for 1 min 92% | 300 adult bees | [5] 1 | ||
Mechanical shaking for 30 min 100% | Approx. 250 adult bees | [6] | ||
Brood uncapping | More reliable in combination with sampling adult bees | 50 worker brood cells and on average 29 drone brood cells | [7] | |
Powdered sugar roll | 91% | Dusting and CO2 anaesthesia, 761 adult bees (5 replicates) | 10 mites/100 bees, 42 h | [8] |
73.8%/90.98%-low infestation level 76.2%/87.86%-medium infestation level 79.8%/82.16%-high infestation level | Approx. 318 adult bees | Sensitivity in lower infestation levels 84.85%, Sensitivity in medium and high infestation level 100% | [9,10] | |
66.10 ± 35.23% and 94.64 ± 9.56% in August and October | 300–400 bees | [4] | ||
CO2 | 49.5% | 200–600 adult bees on average 415 adult bees | At 22 samples from 32 efficiency was below 80% | [11] |
62.5% | On average 427 adult bees | Range 28.6–85.7% | [12] | |
Natural mite fall | Strong linear correlation between natural mite fall and mite fall after chemical treatment r = 0.951 | 22 colonies | [7] | |
Strong linear correlation between natural mite fall and mite fall after chemical treatment r = 0.41–0.89 | 150 colonies | Correlation depending on the year | [13] | |
76.43% low infestation level 68.26% medium infestation level 66.83% high infestation level | Approx. 318 adult bees | [10] |
Apiary ID | Mean Amount of Bees * | Mean Amount of Brood ** | Dispersal Mite Infestation (Mean) *** | Powdered Sugar Roll | Natural Mite Fall | ||
---|---|---|---|---|---|---|---|
Spearman Correlation (p-Value) | Coefficient of Determination | Spearman Correlation (p-Value) | Coefficient of Determination | ||||
ITG1 | 16816 a | 24,880 bc | 146 b | 0.564 (0.030) | 0.319 | 0.839 (<0.0001) | 0.704 |
ITG2 | 12,055 a | 32,746 c | 26 a | 0.623 (0.015) | 0.388 | 0.345 (0.206) | 0.119 |
NA1 | 17,076 a | 19,756 ab | 76 ab | −0.146 (0.604) | 0.021 | −0.072 (0.802) | 0.005 |
NA2 | 13,922 a | 15,293 a | 29 a | −0.462 (0.085) | 0.213 | 0.136 (0.625) | 0.018 |
Total apiaries: 4 | 14,758 | 23,169 | 69 | 0.368 (0.004) | 0.136 | 0.537 (<0.0001) | 0.289 |
Colony Category | Powdered Sugar Roll | Natural Mite Fall |
---|---|---|
Weak | 0.79 (p < 0.05) | 0.66 (p < 0.05) |
Strong | 0.60 (p < 0.05) | 0.74 (p < 0.05) |
Low amount of brood | 0.66 (p < 0.05) | 0.86 (p < 0.05) |
High amount of brood | 0.84 (p < 0.05) | 0.67 (p < 0.05) |
Low infested | 0.67 (p < 0.05) | 0.73 (p < 0.05) |
High infested | 0.48 (p > 0.05) | 0.68 (p < 0.05) |
Apiary ID | Mean Amount of Bees * | Mean Amount of Brood ** | Total Mite Infestation (Mean) *** | Powdered Sugar Roll | Natural Mite Fall | ||
---|---|---|---|---|---|---|---|
Spearman Correlation (p-Value) | Coefficient of Determination | Spearman Correlation (p-Value) | Coefficient of Determination | ||||
DG1 | 19,456 b | 32,733 b | 118 a | 0.267 (0.365) | 0.071 | 0.092 (0.773) | 0.008 |
DG2 | 18,793 b | 28,320 b | 541 b | 0.354 (0.116) | 0.125 | 0.549 (0.011) | 0.302 |
JW1 | 16,697 b | 31,167 b | 131 a | 0.044 (0.879) | 0.002 | 0.846 (<0.001) | 0.715 |
JW2 | 18,061 b | 16,911 a | 179 a | 0.221 (0.296) | 0.049 | 0.084 (0.695) | 0.007 |
CC | 12,843 a | 26,915 b | 2478 b | 0.890 (<0.0001) | 0.791 | 0.926 (<0.0001) | 0.857 |
Total apiaries: 5 | 17,210 | 25,981 | 673 | 0.547 (<0.0001) | 0.299 | 0.625 (<0.0001) | 0.391 |
Colony Category | Powdered Sugar Roll | Natural Mite Fall |
---|---|---|
Weak | 0.77 (p <0.05) | 0.77 (p < 0.05) |
Strong | 0.32 (p < 0.05) | 0.30 (p < 0.05) |
Low amount of brood | 0.58 (p < 0.05) | 0.56 (p < 0.05) |
High amount of brood | 0.44 (p < 0.05) | 0.67 (p < 0.05) |
Low infested | 0.44 (p < 0.05) | 0.26 (p > 0.05) |
High infested | 0.52 (p < 0.05) | 0.68 (p < 0.05) |
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Pietropaoli, M.; Tlak Gajger, I.; Costa, C.; Gerula, D.; Wilde, J.; Adjlane, N.; Aldea-Sánchez, P.; Smodiš Škerl, M.I.; Bubnič, J.; Formato, G. Evaluation of Two Commonly Used Field Tests to Assess Varroa destructor Infestation on Honey Bee (Apis mellifera) Colonies. Appl. Sci. 2021, 11, 4458. https://doi.org/10.3390/app11104458
Pietropaoli M, Tlak Gajger I, Costa C, Gerula D, Wilde J, Adjlane N, Aldea-Sánchez P, Smodiš Škerl MI, Bubnič J, Formato G. Evaluation of Two Commonly Used Field Tests to Assess Varroa destructor Infestation on Honey Bee (Apis mellifera) Colonies. Applied Sciences. 2021; 11(10):4458. https://doi.org/10.3390/app11104458
Chicago/Turabian StylePietropaoli, Marco, Ivana Tlak Gajger, Cecilia Costa, Dariusz Gerula, Jerzy Wilde, Noureddine Adjlane, Patricia Aldea-Sánchez, Maja Ivana Smodiš Škerl, Jernej Bubnič, and Giovanni Formato. 2021. "Evaluation of Two Commonly Used Field Tests to Assess Varroa destructor Infestation on Honey Bee (Apis mellifera) Colonies" Applied Sciences 11, no. 10: 4458. https://doi.org/10.3390/app11104458