Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders
Abstract
:Featured Application
Abstract
1. Introduction
2. Materials and Methods
2.1. Tissue Decellularization
2.2. Decellularization Confirmation
2.3. Biochemical Composition
2.3.1. Collagen and sGAG
2.3.2. Relative Quantification of Protein Profile by Liquid Chromatography–Mass Spectrometry (LC-MS/MS)
2.4. Turbidity Measurement
2.5. Rheological Measurements
2.6. Scanning Electron Microscopy
2.7. Effect on L929 Fibroblast In Vitro Cell Viability
2.8. Effect on C2C12 Myogenic Differentiation
2.8.1. C2C12 Cell Culture
2.8.2. Immunocytochemistry
2.8.3. Western Blot
2.9. Effect on C2C12 Osteoblast Differentiation
2.9.1. Osteoblast Differentiation
2.9.2. Alizarin Red Staining (ARS)
2.9.3. Alkaline Phosphatase (ALP) Activity
2.10. Statistical Analysis
3. Results
3.1. Decellularization Confirmation
3.2. Biochemical Composition
3.3. Turbidity Measurement
3.4. Rheological Measurements
3.5. Scanning Electron Microscopy
3.6. Effect on L292 Fibroblast In Vitro Cell Viability
3.7. Effect on C2C12 Myogenic Differentiation
3.8. Effect on C2C12 Osteoblast Differentiation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kao, C.-Y.; Nguyen, H.-Q.-D.; Weng, Y.-C.; Hung, Y.-H.; Lo, C.-M. Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders. Appl. Sci. 2021, 11, 5820. https://doi.org/10.3390/app11135820
Kao C-Y, Nguyen H-Q-D, Weng Y-C, Hung Y-H, Lo C-M. Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders. Applied Sciences. 2021; 11(13):5820. https://doi.org/10.3390/app11135820
Chicago/Turabian StyleKao, Chen-Yu, Huynh-Quang-Dieu Nguyen, Yu-Chuan Weng, Yu-Han Hung, and Chun-Min Lo. 2021. "Evaluating the Effect of Tissue Selection on the Characteristics of Extracellular Matrix Hydrogels from Decellularized Porcine Bladders" Applied Sciences 11, no. 13: 5820. https://doi.org/10.3390/app11135820