Investigation of Biofabricated Iron Oxide Nanoparticles for Antimicrobial and Anticancer Efficiencies
Round 1
Reviewer 1 Report
Dear authors
I have few reservations before i can further recommend this article.
1. XRD never gives information about size, while in your abstract you wrote size was determined using XRD, which is used to characterize crystallinity and amorphicity of the material. Correct it.
2. No pharmacological effects of iron oxide nanoparticles have been discussed in the introduction section. You are advised to include latest literature regarding use of iron oxide nanoparticles for various pharmacological effects.
3. Please mention the ATCC number for cell line used.
4. Please mention the concentrations used/applied in antimicrobial testing, and also define which applications were used.
5.Discussion is either missing or very limited. Please elaborate it. What happens when you apply iron oxide nps loaded with plant extract on bacteria or cancer cell line? What possible mechanisms might be involved? What you deduced from your FTIR and XRD studies? How it can improve the physicochemical properties of nps which translated into better antibacterial and anticancer effects?
Author Response
As per your suggestion carried out
Author Response File: 
Author Response.docx
Reviewer 2 Report
The manuscript entitled: "Investigation on biofabricated iron oxide nanoparticles for antimicrobial and anticancer efficiencies" from Nilavukkarasi et al, in my opinion, is not recommended for publication, in its present form, in Applied Sciences, requiring a major review. Some of the reasons for drawing this conclusion are as follows:
- Please, pay attention to abbreviations (ex. NPs defined initially, but not used in the manuscript, FTIR, etc.)
- Which are the advantages of using the green synthesis? The authors applied this technique?
- The methods section is very incomplete. For example, the nanoparticles characterization is not described. The evaluation of anticancer activity is also very incomplete.
- Did the authors perform any study of stability of these nanoparticles?
- There is a lack of positive controls for comparison.
- The manuscript has a very poor discussion of the results and the rationale to evaluate these nanoparticles against these pathologies was not discussed.
Author Response
As per your suggestion carried out
Author Response File: Author Response.docx
Round 2
Reviewer 1 Report
Well done.
Reviewer 2 Report
The authors have answered to my remarks and amended, wherever necessary, the text of the manuscript accordingly. At present, I can recommend acceptance of the manuscript for publication.
