Chemical Composition Analysis of Lilium brownii var. Viridulum Baker and the Effect of Postharvest Primary Processing on Its Quality
Round 1
Reviewer 1 Report
The authors had a study on Chemical Composition Analysis of Lilium brownii var. Viridulum Baker and the Effect of Postharvrst Primary Processing on Its Quality.
It represents a main study in its field.The introduction provides sufficient background and includes all relevant references. All the cited references are relevant to the research.
The Figures and Tables are enough and good in quality.
While the study's methodology section is well described, as well as the results obtained including the statistical analysis, some improvement can be made in the manuscript.
What was the sample size determination method?
What were the statistical methods?
What was the validation method?
Author Response
Dear reviewer,
We feel great thanks for your professional review work on our article.
A point-by-point response to comments can find as follows.
- What was the sample size determination method?
Response: Non-probability sampling was adopted in the manuscripts.
- What were the statistical methods?
Response: Multiple comparison was adopted in the manuscripts.
- What was the validation method?
Response: Analysis of variance was adopted in the manuscripts.
Thank you again for your positive and constructive comments and suggestions on our mamuscript.
With Best Regards.
Yours Sincerely
Zhang Sheng
Reviewer 2 Report
This article is comprehensive, logically organized, and contains valuable information on the chemical composition analysis of Lilium brownii var. Viridulum Baker and the effect of post-harvest primary processing on its quality. The authors did excellent research on investigating the chemical composition of Lilium brownii var. Viridulum Baker and the impact of primary processing on its quality, a comparison was made on the contents of polysaccharide and extract of ten batches of Lilium from different regions, including Lilium lancifolium Thunb., Lilium brownii var. Viridulum Baker, and Lilium davidi var. unicdor cotton. The authors demonstrated that the chemical composition of Lilium lancifolium Thunb. and Lilium brownii var. Viridulum Baker is relatively similar. The authors presented the polysaccharide and extract contents of fresh Lilium S9 at different boiling times, and the polysaccharide and extract contents of fresh Lilium S9 at different drying temperatures in Figures 6 and 7, respectively. This manuscript does not contain much of an error analysis on the polysaccharide and extract contents performance which is highly required for readability purposes. It is suggested the authors should place the error bars of the polysaccharide and extract contents performance data as presented in Figures 6 and 7, for the reliability and readability of the present research. The submitted manuscript has significant scientific insights and the conclusions are soundly supported by the experimental data. Therefore, the manuscript requires minor revisions before being accepted in the well-circulated journal, Applied Sciences, in the current form.
Conclusion: A total of ten batches of Lilium from three different sources were collected in this study, of which six batches were Lilium lancifolium Thunb., and three were Lilium brownii var. Viridulum Baker, and one was Lilium davidi var. unicdor cotton. HPLC fingerprinting analysis showed that the similarity between Lilium davidi var. unicdor cotton and other varieties was low, and the chemical composition differences were significant. The chemical composition of Lilium lancifolium Thunb. and Lilium brownii var. Viridulum Baker was relatively similar. According to the UPLC-Q-TOF-MSE analysis method, 70 compounds were identified, including 22 phenolic compounds, 41 steroidal saponins, 6 alkaloids, and 1 flavonoid. The primary processing of fresh Lilium is a critical factor in ensuring its suitability for use in Traditional Chinese Medicine. This study investigated the effects of boiling time and drying temperature on the quality of Lilium based on the content of polysaccharide and extract stipulated by the Chinese Pharmacopoeia and concluded that a boiling time of 5 min and a drying temperature of 85℃ had the least effect on the quality of fresh Lilium.
Author Response
Dear reviewer,
We feel great thanks for your professional review work on our article.
A point-by-point response to comments can find as follows.
- It is suggested the authors should place the error barsof the polysaccharide and extract contents performance data as presented in Figures 6 and 7, for the reliability and readability of the present research.
Response: Thanks to the reviewer, this mistake had been corrected in the manuscript.
- Comments on the Quality of English Language.
Response:The manuscript had been edited for proper English language, grammar, punctuation, spelling, and overall style by one or more highly-qualified native English-speaking editors at Academic Exchange Information Center (AEIC).
Thank you again for your positive and constructive comments and suggestions on our mamuscript.
With Best Regards.
Yours Sincerely
Zhang Sheng
Author Response File: 
Author Response.pdf
Reviewer 3 Report
This paper is on composition analysis of Lilium. Various species collected from diversified geographical regions were studied. The information obtained is new and the paper has some fundamental interest in the plants for agricultural and herbaceous products. The following suggested minor changes are given.
1. Check the words in the title, is "Postharvrst" really "post harvesting"? Also, double check its spelling in the keywords list.
2. On line 203, use subscripts for the numbers in " anhydrous glucose (C6H12O6)".
3. Unify the format in the reference list. For example, the article titles in [17], [9], [18], [21], [10], [7] etc. should be upper- and lower-case letters.
Author Response
Dear reviewer,
We feel great thanks for your professional review work on our article.
A point-by-point response to comments can find as follows.
- Check the words in the title, is "Postharvrst" really "post harvesting"? Also, double check its spelling in the keywords list.
Response: Thanks to the reviewer, this mistake had been corrected in the manuscript.
- On line 203, use subscripts for the numbers in " anhydrous glucose (C6H12O6)".
Response: Thanks to the reviewer, this mistake had been corrected in the manuscript.
- Unify the format in the reference list. For example, the article titles in [17], [9], [18], [21], [10], [7] etc. should be upper- and lower-case letters.
Response: Thanks to the reviewer, this mistake had been corrected in the manuscript.
Thank you again for your positive and constructive comments and suggestions on our mamuscript.
With Best Regards.
Yours Sincerely
Zhang Sheng
