Studies of Fluorescence Lifetimes of Biological Warfare Agents Simulants and Interferers Using the Stroboscopic Method
Abstract
:1. Introduction
2. Materials and Methods
2.1. Measurement Setups
2.2. Fluorescence Lifetime Measurements
2.3. Data Analysis
2.4. Microorganisms
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Compound | EX [nm] | Em [nm] | Lifetime [ns] | Reference |
---|---|---|---|---|
Phe | 257–260 | 282 | 6.4−6.8 | [33,34] |
Tyr | 274−275 | 303−304 | 3.6 | [33,34] |
Trp | 280−295 | 348−353 | 3.1 | [33,34] |
NADH | 346 | 457 | 0.48 | [33,37,38,39,40] |
FAD | 450 | 520 | 2.3−2.9 | [40] |
Riboflavin | 370, 450 | 518 | 5.06 | [20,41,47] |
Folic acid | 361 | 442 | t1 = 1.6 (12%) t2 = 4.78 (88%) | [42] |
FMN | 451 | 520 | 4.66 | [40,43] |
B-Carotene | 471, 498 | 568 | t1 = 0.16 (75%) t2 = 1.07 (25%) | [44,46] |
Pterine | 353 | 440−450 | 5.0−7.6 | [45] |
Biological Sample | Source | Abbrev. |
---|---|---|
Bacillus cereus | ATCC 14579 | BC |
Bacillus cereus spores | ATCC 14579 | BCs |
Bacillus atrophaeus var. globibii | ATCC 9372 | BG |
Bacillus atrophaeus var. globigi spores | ATCC 9372 | BGs |
Bacillus atrophaeus spores technical | MIHE (Warsaw, Poland) | BGst |
Bacillus megaterium | PCM (Wrocław, Poland) 2006 | BM |
Bacillus megaterium spores | PCM (Wrocław, Poland) 2006 | BMs |
Bacillus subtilis | ATCC 6633 | BS |
Bacillus subtilis spores | ATCC 6633 | BSs |
Bacillus thuringiensis | ATCC 10792 | BT |
Bacillus thuringiensis spores | ATCC 10792 | BTs |
Bacillus thuringiensis spores technical | MIHE (Warsaw, Poland) | BTst |
Testing spores | MIHE (Warsaw, Poland) | Sporal S |
Micrococcus luteus | ATCC 4698 | ML |
Escherichia coli | ATCC 25922 | EC |
Alternaria alternata | ATCC 6663 | AA |
Candida albicans | ATCC 18804 | CA |
Cladosporum herbarum | ATCC 28987 | CLH |
Penicillium chrysogenum | ATCC 9197 | PCH |
Penicillium brevicompactum | ATCC 9056 | PBC |
Bermuda grass pollen | Duke Scientific Corp. (Palo Alto, CA, USA) | BER |
Corn pollen | Duke Scientific Corp. (Palo Alto, CA, USA) | COR |
Ragweed pollen | Duke Scientific Corp. (Palo Alto, CA, USA) | RAG |
Secale cereale pollen | Sigma-Aldrich (St. Louis, MO, USA) | SEC |
Paper mulberry pollen | Duke Scientific Corp. (Palo Alto, CA, USA) | PAP |
Artemisia tridentata pollen | Sigma-Aldrich (St. Louis, MO, USA) | ARTT |
Artemisia absinthium pollen | Sigma-Aldrich (St. Louis, MO, USA) | ARTA |
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Kaliszewski, M.; Kwaśny, M.; Bombalska, A.; Włodarski, M.; Trafny, E.A.; Kopczyński, K. Studies of Fluorescence Lifetimes of Biological Warfare Agents Simulants and Interferers Using the Stroboscopic Method. Appl. Sci. 2024, 14, 7332. https://doi.org/10.3390/app14167332
Kaliszewski M, Kwaśny M, Bombalska A, Włodarski M, Trafny EA, Kopczyński K. Studies of Fluorescence Lifetimes of Biological Warfare Agents Simulants and Interferers Using the Stroboscopic Method. Applied Sciences. 2024; 14(16):7332. https://doi.org/10.3390/app14167332
Chicago/Turabian StyleKaliszewski, Miron, Mirosław Kwaśny, Aneta Bombalska, Maksymilian Włodarski, Elżbieta Anna Trafny, and Krzysztof Kopczyński. 2024. "Studies of Fluorescence Lifetimes of Biological Warfare Agents Simulants and Interferers Using the Stroboscopic Method" Applied Sciences 14, no. 16: 7332. https://doi.org/10.3390/app14167332