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Volume 14
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Article

Quinolone and Tetracycline-Resistant Biofilm-Forming Escherichia coli Isolates from Slovak Broiler Chicken Farms and Chicken Meat

by
Nikola Dančová
1,
Gabriela Gregová
1,*,
Tatiana Szabóová
1,
Ivana Regecová
2,
Ján Király
3,
Vanda Hajdučková
3 and
Patrícia Hudecová
3
1
Department of Public Veterinary Medicine and Animal Welfare, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia
2
Department of Food Hygiene, Technology and Safety, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia
3
Department of Microbiology and Immunology, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia
*
Author to whom correspondence should be addressed.
Appl. Sci. 2024, 14(20), 9514; https://doi.org/10.3390/app14209514
Submission received: 24 September 2024 / Revised: 10 October 2024 / Accepted: 16 October 2024 / Published: 18 October 2024
(This article belongs to the Section Applied Microbiology)
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Abstract

Escherichia coli isolates from intensive poultry production are associated with antimicrobial resistance and worldwide health problems. The aim of the study was to detect and evaluate the phenotypic and genotypic antimicrobial resistance, biofilm formation, phylogenetic typing, and virulence factors in E. coli isolates from the rectal swabs of chickens from two farms and swabs of chicken meat purchased from Slovakian food markets. Interpretative readings of minimal inhibitory concentration (MIC) revealed dominant resistance to ampicillin (>50%) in both groups. We also detected higher resistance to ciprofloxacin (45%), tetracycline, ampicillin + sulbactam, and trimethoprim + sulfonamide (each >30%). Here, 28.57% of the strains studied were multidrug-resistant (MDR). The formation of weak biofilms was confirmed in 8.8% of E. coli, while one of the strains obtained from chicken cloacal swabs was classified as a strong biofilm producer. The most frequently confirmed phylogenetic groups in E. coli were B1 and A1 in all groups. PCR detection revealed the presence of genes encoding tetracycline resistance (tetAB) and plasmid-mediated quinolone resistance (qnrABS), and Int1 (52.9%), Tn3 (76.5%), kpsMT II (8.8%), fimA (97.1%), cvaC (38.2%), and iutA (76.5%) genes in the strains studied. Our results demonstrate that chickens and chicken meat were the source of antibiotic-resistant, biofilm-forming, and virulent E. coli, representing a potential risk from the point of view of the One Health concept.
Keywords: antimicrobial resistance; biofilm; Escherichia coli; meat; phylogenetic groups antimicrobial resistance; biofilm; Escherichia coli; meat; phylogenetic groups

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MDPI and ACS Style

Dančová, N.; Gregová, G.; Szabóová, T.; Regecová, I.; Király, J.; Hajdučková, V.; Hudecová, P. Quinolone and Tetracycline-Resistant Biofilm-Forming Escherichia coli Isolates from Slovak Broiler Chicken Farms and Chicken Meat. Appl. Sci. 2024, 14, 9514. https://doi.org/10.3390/app14209514

AMA Style

Dančová N, Gregová G, Szabóová T, Regecová I, Király J, Hajdučková V, Hudecová P. Quinolone and Tetracycline-Resistant Biofilm-Forming Escherichia coli Isolates from Slovak Broiler Chicken Farms and Chicken Meat. Applied Sciences. 2024; 14(20):9514. https://doi.org/10.3390/app14209514

Chicago/Turabian Style

Dančová, Nikola, Gabriela Gregová, Tatiana Szabóová, Ivana Regecová, Ján Király, Vanda Hajdučková, and Patrícia Hudecová. 2024. "Quinolone and Tetracycline-Resistant Biofilm-Forming Escherichia coli Isolates from Slovak Broiler Chicken Farms and Chicken Meat" Applied Sciences 14, no. 20: 9514. https://doi.org/10.3390/app14209514

APA Style

Dančová, N., Gregová, G., Szabóová, T., Regecová, I., Király, J., Hajdučková, V., & Hudecová, P. (2024). Quinolone and Tetracycline-Resistant Biofilm-Forming Escherichia coli Isolates from Slovak Broiler Chicken Farms and Chicken Meat. Applied Sciences, 14(20), 9514. https://doi.org/10.3390/app14209514

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