Application Potential Analysis of Enhanced Oil Recovery by Biopolymer-Producing Bacteria and Biosurfactant-Producing Bacteria Compound Flooding
Round 1
Reviewer 1 Report
Interesting approach for oil recovery in heterogeneous reservoirs, using polymer-producing bacteria FY-07 and the surfactant-producing bacteria WJ-1 has been described. Authors presented comparison studies about oil recovery using each strain separately and both strains in combination.
However, before publication, some points should be addressed:
- How many times experiments were repeated, e.g. did cells counting was carried out at least in triplicate?
- Please, provide name and producer of surface tensiometer.
- Error bars on all graphs are missing.
- Figures 2 and 3 – according to description of the vertical axis (log of cells number) on the scale should be 6, 7, ect. instead of 10^6, 10^7…
- Was physical simulation experiment of oil displacement only conducted at 34°C? Could it be estimated how temperature can affect the test results?
- Developed method has a good application prospects for enhancing the oil recovery in heterogeneous reservoirs. Therefore, could you provide a brief summary in terms of quality of obtained oil. How both strains might influence the composition of crude oil?
Author Response
Please see the attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
In this article, Bi et al. have studied the potential of bacterial cellulose producing bacteria and biosurfactant producing bacteria for enhanced oil recovery. I recommend it for publication once following comments have been well taken care of:
Typo in the reference 17 needs to be corrected. Authors must include these relevant articles to this study (ACS Nano2017, 11, 5, 5148-5159; ACS Nano2018, 12, 7, 6378-6388; ACS Appl. Mater. Interfaces 2017, 9, 23, 19371-19379; doi.org/10.1002/anie.201910603) Error bars must be included in Figure 2. Error bars in Figure 4 are missing. Figure 3 is written as schematic? To be it does not seem like a schematic, it is a figure with absolute data?Author Response
Please see the attachment
Author Response File: Author Response.pdf
