Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities

Martínez de Toda, Irene; Rattan, Suresh I. S.; De la Fuente, Mónica; Arranz, Lorena

doi:10.3390/antiox10091397

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Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities

by

Irene Martínez de Toda

^1,2,

Suresh I. S. Rattan

³

,

Mónica De la Fuente

^1,2 and

Lorena Arranz

^1,*,†

¹

Department of Genetics, Physiology and Microbiology (Unit of Animal Physiology), Faculty of Biology, Complutense University, 28040 Madrid, Spain

²

Institute of Investigation Hospital 12 Octubre, 28041 Madrid, Spain

³

Department of Molecular Biology and Genetics, Århus University, 8000 Århus, Denmark

^*

Author to whom correspondence should be addressed.

^†

Current address: Stem Cells, Ageing and Cancer Research Group, Department of Medical Biology, Faculty of Health Sciences, UiT—The Arctic University of Norway, MH2 Building Level 10, 9019 Tromsø, Norway.

Antioxidants 2021, 10(9), 1397; https://doi.org/10.3390/antiox10091397

Submission received: 28 July 2021 / Revised: 18 August 2021 / Accepted: 28 August 2021 / Published: 31 August 2021

(This article belongs to the Special Issue Oxidative Stress in Aging and Associated Chronic Diseases)

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Abstract

Oxidized, damaged and misfolded proteins accumulate during aging and contribute to impaired cell function and tissue homeodynamics. Damaged proteins are degraded by cellular clearance mechanisms like the 20S proteasome. Aging relates to low 20S proteasome function, whereas long-lived species show high levels. However, contradictory results exist depending on the tissue or cell type and it is unknown how the 20S proteasome functions in exceptionally old mice. The aim of this study was to investigate two proteasome activities (caspase-like and chymotrypsin-like) in several tissues (lung, heart, axillary lymph nodes, liver, kidney) and cells (peritoneal leukocytes) from adult (28 ± 4 weeks, n = 12), old (76 ± 4 weeks, n = 9) and exceptionally old (128 ± 4 weeks, n = 9) BALB/c female mice. The results show different age-related changes depending on the tissue and the activity considered, so there is no universal decline in proteasome function with age in female mice. Interestingly, exceptionally old mice displayed better maintained proteasome activities, suggesting that preserved 20S proteasome is associated with successful aging.

Keywords: aging; healthy aging; exceptionally old; 20S proteasome; caspase-like activity; chymotrypsin-like activity

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MDPI and ACS Style

Martínez de Toda, I.; Rattan, S.I.S.; De la Fuente, M.; Arranz, L. Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities. Antioxidants 2021, 10, 1397. https://doi.org/10.3390/antiox10091397

AMA Style

Martínez de Toda I, Rattan SIS, De la Fuente M, Arranz L. Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities. Antioxidants. 2021; 10(9):1397. https://doi.org/10.3390/antiox10091397

Chicago/Turabian Style

Martínez de Toda, Irene, Suresh I. S. Rattan, Mónica De la Fuente, and Lorena Arranz. 2021. "Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities" Antioxidants 10, no. 9: 1397. https://doi.org/10.3390/antiox10091397

APA Style

Martínez de Toda, I., Rattan, S. I. S., De la Fuente, M., & Arranz, L. (2021). Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities. Antioxidants, 10(9), 1397. https://doi.org/10.3390/antiox10091397

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Female Mice Reaching Exceptionally High Old Age Have Preserved 20S Proteasome Activities

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