Biological Effects of New Hydraulic Materials on Human Periodontal Ligament Stem Cells
Abstract
:1. Introduction
2. Material and Methods
2.1. Material Extracts
2.2. Ion Release Analysis and pH
2.3. Isolation and Culture of hPDLSCs
2.4. Expression of Mesenchymal Stem Cell Surface Markers
2.5. Cell Viability Assay
2.6. Cell Migration
2.7. Analysis of Apoptosis and Necrosis by Flow Cytometry (Annexin V/7-AAD Staining)
2.8. Scanning Electronic Microscopy and Energy-Dispersive X-Ray Analysis (SEM/EDX)
2.9. Statistical Analysis
3. Results
3.1. Ion Release and pH
3.2. Isolation and Identification of hPDLSCs
3.3. MTT Assay
3.4. Migration Assay
3.5. Apoptosis/Necrosis Assay
3.6. Scanning Electronic Microscopy and Energy-Dispersive X-ray Analysis (SEM/EDX)
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Materials | Manufacturer | Composition | Lot Number |
---|---|---|---|
BIO-C Sealer | Angelus, Rua Waldir Landgraf, Barrio Lindóia, Londrina, Brasil. | Powder: calcium silicate (Ca2SiO4), calcium oxide (CaO), zirconium oxide (ZrO2), iron oxide, silicon dioxide (SiO2), and dispersing agent. Dispersion agent/solid ratio = 0.52 | 43,207 |
BIO-C Repair | Angelus, Rua Waldir Ladgraf, Barrio Lindóia, Londrina, Brasil. | Powder: calcium silicate (Ca2SiO4),calcium oxide (CaO), zirconium oxide (ZrO2), iron oxide, silicon dioxide (SiO2), and dispersing agent Dispersion agent/solid ratio = 0.46 | 43,210 |
Concentration of Element (mg/L Solution) | ||||||
---|---|---|---|---|---|---|
pH | Silicon | Strontium | Calcium | Zirconium | ||
Complete DMEM | 7.61 ± 0.03 | Milli-Q water | 0 ± 0 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
Bio-C Sealer | 8.40 ± 0.05 | Bio-C Sealer | 42.01 ± 0.01 | 0.3 ± 0.04 | 63.87 ± 0.01 | 0.13 ± 0.01 |
Bio-C Repair | 8.33 ± 0.02 | Bio-C Repair | 14.90 ± 0.1 | 0.52 ± 0.01 | 38.32 ± 0.02 | 0.12 ± 0.02 |
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López-García, S.; Lozano, A.; García-Bernal, D.; Forner, L.; Llena, C.; Guerrero-Gironés, J.; Moraleda, J.M.; Murcia, L.; Rodríguez-Lozano, F.J. Biological Effects of New Hydraulic Materials on Human Periodontal Ligament Stem Cells. J. Clin. Med. 2019, 8, 1216. https://doi.org/10.3390/jcm8081216
López-García S, Lozano A, García-Bernal D, Forner L, Llena C, Guerrero-Gironés J, Moraleda JM, Murcia L, Rodríguez-Lozano FJ. Biological Effects of New Hydraulic Materials on Human Periodontal Ligament Stem Cells. Journal of Clinical Medicine. 2019; 8(8):1216. https://doi.org/10.3390/jcm8081216
Chicago/Turabian StyleLópez-García, Sergio, Adrián Lozano, David García-Bernal, Leopoldo Forner, Carmen Llena, Julia Guerrero-Gironés, José M. Moraleda, Laura Murcia, and Francisco J. Rodríguez-Lozano. 2019. "Biological Effects of New Hydraulic Materials on Human Periodontal Ligament Stem Cells" Journal of Clinical Medicine 8, no. 8: 1216. https://doi.org/10.3390/jcm8081216