QTL Mapping for Fiber Quality Based on Introgression Lines Population from G. hirsutum × G. tomentosum
, Chao Shen 2, Lei Chao 1, Guangling Shui 1, Chunyuan You 3,4, Jianwei Xu 1,*, Zhongxu Lin 1,3,* and Xinhui Nie 1,*Round 1
Reviewer 1 Report
I have critically reviewed the article and the methods mentioned in this research are up to date with the current methodology and recent techniques used for QTL mapping. So technically data given in this manuscript is sound in its field.
1. All the scientific names should be in italic.
2. Give one picture indicating the polymorphism during genotyping
Author Response
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Reviewer 2 Report
Many queries raised, see attached pdf and resolve them
Comments for author File: Comments.pdf
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Reviewer 3 Report
QTL Mapping for Fiber Quality Based on Introgression Lines Population from G. hirusutum X G. Tomentosum
1. Grammatical errors throughout the MS, flow of English is not so good. Please make necessary changes. For ex. Lines 38-39 “economically important crops in China, which acts an important role in national economy”. In few places sentence starts with “And” which I find is not appropriate way to start a new sentence. For ex. Line 20 and 80. Line 162 “to uncovered”.
2. Location name spelling is different in different places “Manas” in line 92 and “Manasi” in Fig 2 legend. Correct accordingly.
3. Fig 1. Should not be in materials and method section.
4. Sequencing depth is not mentioned accurately its just written “more than 20 times” it doesn’t makes sense. It can be number of times, be specific. (Line128-129). Sequencing statistics should be mention in main text or in supplementary file.
5. Lines 129-131, how did you reached to final 3,157 number of SNPs, mention the criteria for filtering SNPs. Was binning performed? Were similar individuals were removed?? SDR analysis was perfomed?
6. Line 136: What does “Genotyping of SNP according to QTLIciMapping 4.2 software” means, I think should be scoring not genotyping. Please check and correct.
7. In section 2.6 Primer3Plus information is mentioned in paragraph ending, it should be near to line no. 151. Also, mention the full name of reference gene.
8. Table S2 should be in main text.
9. Line 225 you have mentioned genetic map has been constructed using JoinMap, but in Materials and methods it is not mentioned. Why you used joinmap as you can make genetic map in ICimapping software also, where you did QTL mapping?
10. In Table 2 add information about Average marker distance and Max interval also.
11. In Table 3, also add columns for marker interval, marker start and end position, Flanking markers, Dominant and main effect for each QTL detected.
12. As you have performed QTL analysis in different environment, have you performed genotype x environment (GXE) study? You should have also mentioned about the effect of different environment on detected QTLs and should have made a conclusive QTL analysis for detecting stable QTLs.
13. In line 303, you have mentioned about 9 homologous genes, but in text can not see details about all the nine genes. Please mention about all of them.
14.In Fig. 6 you have used a reference gene as control, but in figure there is no mention of control. Without control this fig is incomplete.
Author Response
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Reviewer 4 Report
The article entitled "QTL Mapping for Fiber Quality Based on Introgression Lines 2
Population from G. hirsutum × G. tomentosum" is well compiled manuscript, and the authors performed SLAF-seq on a introgression population to construct a high-density genetic map, and screened the candidate genes by QTL mapping on fiber quality. In general, the results are innovative, significant and useful for the cotton research. However, several technical issues should be addressed first.
(1) The writing of the manuscript needs improvement, and some grammatical and spelling errors still exist. Suggest to invite professional editors to help with the polish.
(2) Line 57, no need to italic for L.
(3) Line 163-165, the authors presented a lot of replications as the described methods, and please re-checked the other results.
(4) Why the authors not utilized the reference “Gossypium tomentosum genome and interspecific ultra-dense genetic maps reveal genomic structures, recombination landscape and flowering depression in cotton”, and please confirm if the candidate genes showed the sequence differences between Gossypium tomentosum and G. hirsutum.
(5) The formats of references in this manuscript were not uniform, and some Latin names were not italic.
Author Response
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