Surfactant-Assisted Label-Free Fluorescent Aptamer Biosensors and Binding Assays
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Effect of Time-Dependent Stabilization
2.3. Test of Different Surfactants and Assay Materials
2.4. Hg2+ Sensing Assay
2.5. Adenosine Sensing Assay
2.6. Cortisol Sensing Assay
3. Results and Discussion
3.1. Effect of Stabilization in Microplates and Cuvettes
3.2. Time-Dependent Signal Stability
3.3. The Protective Effect of Surfactants
3.4. Sensing Mercury Using a Thymine-Rich DNA
3.5. Evaluation of the Adenosine Aptamer
3.6. Evaluation of the Cortisol Aptamer
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Name | Sequences (5′-3′) |
---|---|
DNA1 | ACGACACGGAGGCTTAGTTTGCTAAATGGTCATGTCGT |
CSS.1-42 | GACGACGCCCGCATGTTCCATGGATAGTCTTGACTAGTCGTC |
Ade Apt | ACCTGGGGGAGTATTGCGGAGGAAGGT |
Ade Apt M2 | ACCTGGGGTAGTATTGCGGAGTAAGGT |
T30 | TTTTTTTTTTTTTTTTTTTTTTTTTTTTTT |
Surfactant | Molecular Weight | Concentration at 0.01% (mM) | CMC (mM) |
---|---|---|---|
Triton X-100 | 646.9 | 0.155 | 0.19–0.22 |
TWEEN 20 | 522.7 | 0.191 | 0.046 |
SDS | 288.4 | 0.347 | 7.3–8.1 |
CTAB | 364.5 | 0.274 | 0.98 |
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Zhang, H.; Li, A.Z.; Liu, J. Surfactant-Assisted Label-Free Fluorescent Aptamer Biosensors and Binding Assays. Biosensors 2023, 13, 434. https://doi.org/10.3390/bios13040434
Zhang H, Li AZ, Liu J. Surfactant-Assisted Label-Free Fluorescent Aptamer Biosensors and Binding Assays. Biosensors. 2023; 13(4):434. https://doi.org/10.3390/bios13040434
Chicago/Turabian StyleZhang, Hanxiao, Albert Zehan Li, and Juewen Liu. 2023. "Surfactant-Assisted Label-Free Fluorescent Aptamer Biosensors and Binding Assays" Biosensors 13, no. 4: 434. https://doi.org/10.3390/bios13040434