Age-Dependence and Aging-Dependence: Neuronal Loss and Lifespan in a C. elegans Model of Parkinson’s Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Parkinson’s Disease Model
2.2. General Methods and Strains
2.3. Construction of Strains
2.4. Lifespan Assays
2.5. Microscopy
2.6. Computational Power Analysis
2.7. Statistical Analysis
3. Results
3.1. Lifespan and Neurodegeneration are the Outcomes of Independent Processes
3.2. Tissue-Specific Insulin/IGF1 Signaling Has Neuron-Specific Effects on Age-Dependent Dopaminergic Loss
3.3. After a Lifespan-Extending Intervention, Lifespan and Neurodegeneration Are Still Independent
4. Discussion
4.1. Age-Dependence of Neuronal Loss in a C. elegans Parkinson’s Disease Model
4.2. Aging-Independence of Neuronal Loss in a C. elegans Parkinson’s Disease Model
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Apfeld, J.; Fontana, W. Age-Dependence and Aging-Dependence: Neuronal Loss and Lifespan in a C. elegans Model of Parkinson’s Disease. Biology 2018, 7, 1. https://doi.org/10.3390/biology7010001
Apfeld J, Fontana W. Age-Dependence and Aging-Dependence: Neuronal Loss and Lifespan in a C. elegans Model of Parkinson’s Disease. Biology. 2018; 7(1):1. https://doi.org/10.3390/biology7010001
Chicago/Turabian StyleApfeld, Javier, and Walter Fontana. 2018. "Age-Dependence and Aging-Dependence: Neuronal Loss and Lifespan in a C. elegans Model of Parkinson’s Disease" Biology 7, no. 1: 1. https://doi.org/10.3390/biology7010001