The Impact of Microbiome and Microbiota-Derived Sodium Butyrate on Drosophila Transcriptome and Metabolome Revealed by Multi-Omics Analysis
Abstract
:1. Introduction
2. Results
2.1. Drosophila Microbiome and Metabolites Regulate Host Gene Expression under Sterile Condition
2.2. Drosophila Microbiome and Metabolites Regulate Host Gene Expression under Conventional Condition
3. Discussion
4. Materials and Methods
4.1. Conventional Drosophila and Sterile Drosophila
4.2. Gut Microbiome Analysis
4.3. RNA Sequencing and Data Analysis
4.4. Metabolites Extraction, UHPLC-MS/MS and Metabolome Analysis
4.5. RNA Preparation and RT-qPCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Zhou, F.; Liu, B.; Liu, X.; Li, Y.; Wang, L.; Huang, J.; Luo, G.; Wang, X. The Impact of Microbiome and Microbiota-Derived Sodium Butyrate on Drosophila Transcriptome and Metabolome Revealed by Multi-Omics Analysis. Metabolites 2021, 11, 298. https://doi.org/10.3390/metabo11050298
Zhou F, Liu B, Liu X, Li Y, Wang L, Huang J, Luo G, Wang X. The Impact of Microbiome and Microbiota-Derived Sodium Butyrate on Drosophila Transcriptome and Metabolome Revealed by Multi-Omics Analysis. Metabolites. 2021; 11(5):298. https://doi.org/10.3390/metabo11050298
Chicago/Turabian StyleZhou, Fan, Biaodi Liu, Xin Liu, Yan Li, Luoluo Wang, Jia Huang, Guanzheng Luo, and Xiaoyun Wang. 2021. "The Impact of Microbiome and Microbiota-Derived Sodium Butyrate on Drosophila Transcriptome and Metabolome Revealed by Multi-Omics Analysis" Metabolites 11, no. 5: 298. https://doi.org/10.3390/metabo11050298