High-Throughput Chlorophyll and Carotenoid Profiling Reveals Positive Associations with Sugar and Apocarotenoid Volatile Content in Fruits of Tomato Varieties in Modern and Wild Accessions
Abstract
:1. Introduction
2. Results
2.1. Method Development for High-Throughput Quantification of Carotenoids, Lycopene, and Chlorophylls in Tomato Fruits
2.2. Pigment-Associated Characterization of 157 Varieties of Tomato Fruit
2.3. Association between Pigments and Flavor-Related Compounds
2.4. Evaluation of Relationships between Fruit Color Variation, Carotenoid Composition, and AC-VOC Content in Eight Representative Varieties
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Plant Materials and Sample Collection
4.3. Extraction and High-Throughput Measurement for Quantification of the Targeted Pigments Using Microplate Reader
4.4. Calculation of Carotenoid and Chlorophyll Content
4.5. Pigment Extraction and Carotenoid Profiling Using HPLC
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Aono, Y.; Asikin, Y.; Wang, N.; Tieman, D.; Klee, H.; Kusano, M. High-Throughput Chlorophyll and Carotenoid Profiling Reveals Positive Associations with Sugar and Apocarotenoid Volatile Content in Fruits of Tomato Varieties in Modern and Wild Accessions. Metabolites 2021, 11, 398. https://doi.org/10.3390/metabo11060398
Aono Y, Asikin Y, Wang N, Tieman D, Klee H, Kusano M. High-Throughput Chlorophyll and Carotenoid Profiling Reveals Positive Associations with Sugar and Apocarotenoid Volatile Content in Fruits of Tomato Varieties in Modern and Wild Accessions. Metabolites. 2021; 11(6):398. https://doi.org/10.3390/metabo11060398
Chicago/Turabian StyleAono, Yusuke, Yonathan Asikin, Ning Wang, Denise Tieman, Harry Klee, and Miyako Kusano. 2021. "High-Throughput Chlorophyll and Carotenoid Profiling Reveals Positive Associations with Sugar and Apocarotenoid Volatile Content in Fruits of Tomato Varieties in Modern and Wild Accessions" Metabolites 11, no. 6: 398. https://doi.org/10.3390/metabo11060398