Comparative Transcriptome Analysis of Two Chrysomycin-Producing Wild-Type and Mutant Strains of Streptomyces sp. 891
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microbes and Cultivation
2.2. RNA Extraction
2.3. Illumina Sequencing, Assembly, and Annotation
2.4. Bioinformatics Analysis
3. Results
3.1. Morphological Features of Strains 891 and 891-B6
3.2. RNA-Seq Gene Expression Analysis
3.3. Analyses of DEGs and Identification of Regulated Genes
3.4. GO and KEGG Pathway Enrichment Analyses of DEGs
3.5. Analyses of SNPs and InDels
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene ID | Gene Name | LogFC | p-Value | Proposed Function |
---|---|---|---|---|
gene 5694 | chryE | 3.79 | 0.00 | putative TDP-glucose-4,6-dehydratase |
gene 5695 | chryD | 4.27 | 0.00 | putative glucose-1-phosphate thymidylyltransferase |
gene 5696 | chryXa | 3.00 | 0.00 | unknow |
gene 5697 | chryGT | 4.72 | 0.00 | putative C-glycosyltransferase |
gene 5698 | chryMT | 4.90 | 0.00 | putative O-methyltransferase |
gene 5699 | chryOII | 5.61 | 0.00 | putative anthrone monooxygenase |
gene 5700 | chryRM | 5.40 | 0.00 | putative C-methyltransferase |
gene 5701 | chryRM | 4.86 | 0.01 | putative C-methyltransferase |
gene 5702 | chryU | - | 0.00 | putative NDP-hexose 4-ketoreductase |
gene 5703 | chryCMT | 5.40 | 0.00 | putative C-methyltransferase |
gene 5704 | chryF | 4.41 | 0.00 | putative polyketide ketoreductase |
gene 5705 | chryV | 4.71 | 0.00 | hypothetical protein |
gene 5706 | chryH | 4.44 | 0.01 | putative oxidoreductase/NADH-dependent FMN reductase |
gene 5707 | chryOI | 5.69 | 0.00 | putative FAD-dependent monooxygenase |
gene 5708 | chryG | 6.01 | 0.00 | putative cyclase |
gene 5710 | chryK | 5.16 | 0.00 | putative bifunctional cyclase/dehydratase |
gene 5711 | chryOIV | 5.06 | 0.00 | putative FAD-dependent monooxygenase |
gene 5712 | chryA | 4.33 | 0.00 | putative ketoacyl synthase (KSα) |
gene 5713 | chryB | 8.07 | 0.00 | putative chain length factor (KSβ) |
gene 5714 | chryC | - | 0.01 | putative acyl carrier protein |
gene 5715 | chryOIII | 5.06 | 0.00 | putative P450 monooxygenase |
gene 5717 | chryP | 4.23 | 0.00 | putative malonyl-CoA carrier protein transacylase |
gene 5718 | chryQ | 5.37 | 0.00 | putative propionyl-CoA carrier protein transacylase |
gene 5723 | chryX1 | 4.63 | 0.02 | putative spore-associated protein precursor |
gene 5724 | - | 5.49 | 0.00 | unknown |
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Zhu, W.; Pei, X.; Chen, X.; Wu, Y.; Song, F.; Zhang, H. Comparative Transcriptome Analysis of Two Chrysomycin-Producing Wild-Type and Mutant Strains of Streptomyces sp. 891. Metabolites 2022, 12, 1170. https://doi.org/10.3390/metabo12121170
Zhu W, Pei X, Chen X, Wu Y, Song F, Zhang H. Comparative Transcriptome Analysis of Two Chrysomycin-Producing Wild-Type and Mutant Strains of Streptomyces sp. 891. Metabolites. 2022; 12(12):1170. https://doi.org/10.3390/metabo12121170
Chicago/Turabian StyleZhu, Wangjie, Xinwei Pei, Xiaoyu Chen, You Wu, Fuhang Song, and Huawei Zhang. 2022. "Comparative Transcriptome Analysis of Two Chrysomycin-Producing Wild-Type and Mutant Strains of Streptomyces sp. 891" Metabolites 12, no. 12: 1170. https://doi.org/10.3390/metabo12121170