Next Article in Journal
Muscle Pathology in Dystrophic Rats and Zebrafish Is Unresponsive to Taurine Treatment, Compared to the mdx Mouse Model for Duchenne Muscular Dystrophy
Next Article in Special Issue
Inhibiting Angiogenesis by Anti-Cancer Saponins: From Phytochemistry to Cellular Signaling Pathways
Previous Article in Journal
Metabolites That Confirm Induction and Release of Dormancy Phases in Sweet Cherry Buds
Previous Article in Special Issue
Natural Steroidal Lactone Induces G1/S Phase Cell Cycle Arrest and Intrinsic Apoptotic Pathway by Up-Regulating Tumor Suppressive miRNA in Triple-Negative Breast Cancer Cells
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Metabolites
Volume 13
Issue 2
10.3390/metabo13020229
metabolites-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Quercetin Induces Apoptosis in HepG2 Cells via Directly Interacting with YY1 to Disrupt YY1-p53 Interaction

by
Hui Guan
1,
Wenyuan Zhang
1,
Hui Liu
1,
Yang Jiang
1,
Feng Li
1,
Maoyu Wu
2,
Geoffrey I. N. Waterhouse
3,
Dongxiao Sun-Waterhouse
1,3,* and
Dapeng Li
1,*
1
Key Laboratory of Food Processing Technology and Quality Control of Shandong Higher Education Institutes, College of Food Science and Engineering, Shandong Agricultural University, 61 Dai Zong Street, Tai’an 271018, China
2
Jinan Fruit Research Institute of All China Federation of Supply & Marketing Cooperatives, 16001 East Road Jingshi, Jinan 250220, China
3
School of Chemical Sciences, The University of Auckland, Auckland 0632, New Zealand
*
Authors to whom correspondence should be addressed.
Metabolites 2023, 13(2), 229; https://doi.org/10.3390/metabo13020229
Submission received: 29 December 2022 / Revised: 28 January 2023 / Accepted: 1 February 2023 / Published: 3 February 2023
(This article belongs to the Special Issue Effects of Plant Active Components on Cell Metabolism and Their Anti-tumor Properties)
Browse Figures
Versions Notes

Abstract

Quercetin is a flavonol found in edible plants and possesses a significant anticancer activity. This study explored the mechanism by which quercetin prevented liver cancer via inducing apoptosis in HepG2 cells. Quercetin induced cell proliferation and apoptosis through inhibiting YY1 and facilitating p53 expression and subsequently increasing the Bax/Bcl-2 ratio. The results revealed that YY1 knockdown promoted apoptosis, whilst YY1 overexpression suppressed apoptosis via direct physical interaction between YY1 and p53 to regulate the p53 signaling pathway. Molecular docking using native and mutant YY1 proteins showed that quercetin could interact directly with YY1, and the binding of quercetin to YY1 significantly decreased the docking energy of YY1 with p53 protein. The interactions between quercetin and YY1 protein included direct binding and non-bonded indirect interactions, as confirmed by cellular thermal shift assay, UV-Vis absorption spectroscopy, fluorescence spectroscopy and circular dichroism spectroscopy. It was likely that quercetin directly bound to YY1 protein to compete with p53 for the binding sites of YY1 to disrupt the YY1-p53 interaction, thereby promoting p53 activation. This study provides insights into the mechanism underlying quercetin’s anticancer action and supports the development of quercetin as an anticancer therapeutic agent.
Keywords: quercetin-YY1 interaction; anticancer; apoptosis; YY1-p53 binding; binding competition quercetin-YY1 interaction; anticancer; apoptosis; YY1-p53 binding; binding competition
Graphical Abstract

Share and Cite

MDPI and ACS Style

Guan, H.; Zhang, W.; Liu, H.; Jiang, Y.; Li, F.; Wu, M.; Waterhouse, G.I.N.; Sun-Waterhouse, D.; Li, D. Quercetin Induces Apoptosis in HepG2 Cells via Directly Interacting with YY1 to Disrupt YY1-p53 Interaction. Metabolites 2023, 13, 229. https://doi.org/10.3390/metabo13020229

AMA Style

Guan H, Zhang W, Liu H, Jiang Y, Li F, Wu M, Waterhouse GIN, Sun-Waterhouse D, Li D. Quercetin Induces Apoptosis in HepG2 Cells via Directly Interacting with YY1 to Disrupt YY1-p53 Interaction. Metabolites. 2023; 13(2):229. https://doi.org/10.3390/metabo13020229

Chicago/Turabian Style

Guan, Hui, Wenyuan Zhang, Hui Liu, Yang Jiang, Feng Li, Maoyu Wu, Geoffrey I. N. Waterhouse, Dongxiao Sun-Waterhouse, and Dapeng Li. 2023. "Quercetin Induces Apoptosis in HepG2 Cells via Directly Interacting with YY1 to Disrupt YY1-p53 Interaction" Metabolites 13, no. 2: 229. https://doi.org/10.3390/metabo13020229

APA Style

Guan, H., Zhang, W., Liu, H., Jiang, Y., Li, F., Wu, M., Waterhouse, G. I. N., Sun-Waterhouse, D., & Li, D. (2023). Quercetin Induces Apoptosis in HepG2 Cells via Directly Interacting with YY1 to Disrupt YY1-p53 Interaction. Metabolites, 13(2), 229. https://doi.org/10.3390/metabo13020229

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop