Effects of Water Availability in the Soil on Tropane Alkaloid Production in Cultivated Datura stramonium
Abstract
:1. Introduction
2. Results
2.1. Water Availability in Roots Depending on Irrigation
2.2. Profile of Tropane Alkaloids
2.2.1. Metabolite Family Hypotheses
2.2.2. Manual Interpretation of Mass Spectra and Relative Signal Quantitation
2.3. Absolute Concentration of Atropine and Scopolamine
2.4. Atropine Hydroxylation
2.5. Elemental Nitrogen Content
3. Discussion
3.1. Effect of Irrigation on Tropane Alkaloid Accumulation
3.2. Total Tropane Alkaloid Productivity
3.3. Aromatic Tropane Alkaloid Metabolism
4. Materials and Methods
4.1. Plant Material and Greenhouse Conditions
4.2. Irrigation/Tensiometers
4.3. Metabolite Extraction
4.4. LC-MS Metabolic Profiling and MS2
4.5. LC-MS Data Processing
4.6. Interpretation of Mass Spectra and Generation of Compound Structure Hypotheses
4.6.1. Processing of MS2 Mass Spectra with MetFamily
4.6.2. Manual Interpretation of Mass Spectra and Ion Signal Relative Quantitation
4.7. Quantification of Atropine and Scopolamine
4.8. Quantification of Elemental Nitrogen
4.9. Additional Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Moreno-Pedraza, A.; Gabriel, J.; Treutler, H.; Winkler, R.; Vergara, F. Effects of Water Availability in the Soil on Tropane Alkaloid Production in Cultivated Datura stramonium. Metabolites 2019, 9, 131. https://doi.org/10.3390/metabo9070131
Moreno-Pedraza A, Gabriel J, Treutler H, Winkler R, Vergara F. Effects of Water Availability in the Soil on Tropane Alkaloid Production in Cultivated Datura stramonium. Metabolites. 2019; 9(7):131. https://doi.org/10.3390/metabo9070131
Chicago/Turabian StyleMoreno-Pedraza, Abigail, Jennifer Gabriel, Hendrik Treutler, Robert Winkler, and Fredd Vergara. 2019. "Effects of Water Availability in the Soil on Tropane Alkaloid Production in Cultivated Datura stramonium" Metabolites 9, no. 7: 131. https://doi.org/10.3390/metabo9070131