p19Arf Exacerbates Cigarette Smoke-Induced Pulmonary Dysfunction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. CS and CSE Treatment
2.3. In Vivo Imaging Analysis
2.4. Morphometric Analysis
2.5. Immunofluorescence
2.6. RNA Analysis
2.7. Pulmonary Function Test
2.8. Bronchoalveolar Lavage Fluid (BALF) Analysis
3. Results
3.1. Cigarette Smoking Induces Arf and Ink4a Expression in Mice
3.2. Ablation of Arf Expression Attenuates CS-Induced Lung Pathologies
3.3. Arf-Expressing Cells Facilitates CS-Induced Pulmonary Dysfunction
3.4. Effects of p19Arf-Expressing Cell Ablation in Cigarette Smoke Extract-Treated Mice
3.5. CSE Induced Pulmonary Dysfunction in Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Mikawa, R.; Sato, T.; Suzuki, Y.; Baskoro, H.; Kawaguchi, K.; Sugimoto, M. p19Arf Exacerbates Cigarette Smoke-Induced Pulmonary Dysfunction. Biomolecules 2020, 10, 462. https://doi.org/10.3390/biom10030462
Mikawa R, Sato T, Suzuki Y, Baskoro H, Kawaguchi K, Sugimoto M. p19Arf Exacerbates Cigarette Smoke-Induced Pulmonary Dysfunction. Biomolecules. 2020; 10(3):462. https://doi.org/10.3390/biom10030462
Chicago/Turabian StyleMikawa, Ryuta, Tadashi Sato, Yohei Suzuki, Hario Baskoro, Koichiro Kawaguchi, and Masataka Sugimoto. 2020. "p19Arf Exacerbates Cigarette Smoke-Induced Pulmonary Dysfunction" Biomolecules 10, no. 3: 462. https://doi.org/10.3390/biom10030462