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Biomolecules
Volume 10
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10.3390/biom10050724
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Article

Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp

by
Agnieszka Wilkowska
1,*,
Joanna Berlowska
2,
Adriana Nowak
2,
Ilona Motyl
2,
Aneta Antczak-Chrobot
3,
Maciej Wojtczak
3,
Alina Kunicka-Styczyńska
1,
Michał Binczarski
4 and
Piotr Dziugan
2
1
Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Science, Łódź University of Technology, Wólczańska 171/173, 90-924 Łódź, Poland
2
Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, Poland
3
Institute of Technology and Analysis of Food, Faculty of Biotechnology and Food Science, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, Poland
4
Institute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Żeromskiego 116, 90-924 Łódź, Poland
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(5), 724; https://doi.org/10.3390/biom10050724
Submission received: 28 February 2020 / Revised: 3 May 2020 / Accepted: 4 May 2020 / Published: 6 May 2020
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Abstract

An effective and ecological method for liberation of pectin-derived oligosaccharides (POS) from sugar beet pulp (SBP) was developed using enzymatic and microorganism-mediated biomass conversion. The POS may be applied in the production of prebiotic feed additives. Various yeast strains were screened for their capacity for protein synthesis and monosaccharide assimilation. Combined yeast cultivation and pectin hydrolysis were found to be an effective method of producing prebiotics. Separate enzymatic hydrolysis and fermentation of SBP resulted in the release of 3.6 g of POS per 100 g d.w., whereas the yield of POS acquired after the combined process was 17.9% higher, giving 4.2 g of POS per 100 g d.w. Introducing the yeast into the process improved hydrolysis performance due to lower enzyme inhibition by mono- and disaccharides. The prebiotic effect of the POS was assessed by in vitro fermentation using individual cultures of gastrointestinal bacteria. The POS in the SBP hydrolysate effectively promoted the growth of lactobacilli and bifidobacteria. A large increase in adherence to Caco-2 cells in the presence of POS was noted for beneficial Lactobacillus brevis strains, whereas pathogenic bacteria and yeast (C. albicans, C. lusitanie, C. pelliculosa), responsible for infections in breeding animals, showed much weaker adhesion.
Keywords: pectin-derived oligosaccharides (POS); prebiotics; sugar beet pulp; animal feed pectin-derived oligosaccharides (POS); prebiotics; sugar beet pulp; animal feed

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MDPI and ACS Style

Wilkowska, A.; Berlowska, J.; Nowak, A.; Motyl, I.; Antczak-Chrobot, A.; Wojtczak, M.; Kunicka-Styczyńska, A.; Binczarski, M.; Dziugan, P. Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp. Biomolecules 2020, 10, 724. https://doi.org/10.3390/biom10050724

AMA Style

Wilkowska A, Berlowska J, Nowak A, Motyl I, Antczak-Chrobot A, Wojtczak M, Kunicka-Styczyńska A, Binczarski M, Dziugan P. Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp. Biomolecules. 2020; 10(5):724. https://doi.org/10.3390/biom10050724

Chicago/Turabian Style

Wilkowska, Agnieszka, Joanna Berlowska, Adriana Nowak, Ilona Motyl, Aneta Antczak-Chrobot, Maciej Wojtczak, Alina Kunicka-Styczyńska, Michał Binczarski, and Piotr Dziugan. 2020. "Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp" Biomolecules 10, no. 5: 724. https://doi.org/10.3390/biom10050724

APA Style

Wilkowska, A., Berlowska, J., Nowak, A., Motyl, I., Antczak-Chrobot, A., Wojtczak, M., Kunicka-Styczyńska, A., Binczarski, M., & Dziugan, P. (2020). Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp. Biomolecules, 10(5), 724. https://doi.org/10.3390/biom10050724

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