Natural Extracts to Augment Energy Expenditure as a Complementary Approach to Tackle Obesity and Associated Metabolic Alterations
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines and Reagents
2.2. Natural Plant-Derived Extracts
2.3. Cell Culture
2.3.1. Cell Culture of Human HEK293
2.3.2. Human Adipocytes Cell Culture and In Vitro Differentiation
2.3.3. Human Myocytes Cell Culture and In Vitro Differentiation
2.4. MTT Assay
2.5. Gene Expression Analysis
2.6. Lipid Accumulation: Oil Red O Staining
2.7. Analysis of Mitochondrial Respiration
2.8. Data and Statistical Analysis
3. Results
3.1. Screening of Plant-Derived Extracts in HEK293T Cell Line: MTT Assay and Gene Expression of Selected Metabolic Pathway
3.2. Screening of Plant-Derived Extracts in the Expression of Genes Implicated in the Activation of Thermogenesis and Mitochondrial Oxidative Phosphorylation
3.2.1. Effect of the Extracts on Cell Viability of Human Pre-Adipocytes and Human My-Blasts
3.2.2. Effect of the Extracts in the Expression of Genes Related to the Activation of Thermogenesis and Mitochondrial Oxidative Phosphorylation
3.2.3. Functional Analysis of Mitochondrial Oxidative Phosphorylation and Lipid Accumulation in Differentiated Adipocytes
3.3. Functional Analysis of Mitochondrial Oxidative Phosphorylation in Differentiated Myocytes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Terpenes | Saponins | Aesculus hippocastanum L. (Horse chesnut) | Seed | Ethanol: water (70:30 V/V) | Aescin | 10, 30, 90 | >100 |
Panax ginseng C.A.Mey (Ginseng) | Root | Ethanol: water (50:50 V/V) | Ginsenoside | 10, 30, 90 | >100 | ||
Diterpenes | Rosmarinus officinalis L. (Rosemary) | Leaves | Ethanol | Carnosic acid | 5, 10, 15 | ≈18 | |
Organic acids | Garcinia Cambogia L. (Garcinia) | Fruit | Water | Hydroxycitric acid | 10, 30, 90 | >100 | |
Polyphenols | Phenolic acids | Cynara scolymus L. (Artichoke) | Leaves | Ethanol: water (60:40 V/V) | Hydroxycinnamic derivatives | 10, 30, 90 | >100 |
Coffea Arabica L. (Green coffee) | Fruit | Ethanol: water (60:40 V/V) | Hydroxycinnamic derivatives | 10, 30, 90 | >100 | ||
Punica granatum L. (Pomegranate) | Fruit | Ethanol: water (70:30 V/V) | Punicalagin | 10, 30, 90 | >100 | ||
Olea europaea L. (Olive) | Fruit | Water | Hydroxytyrosol | 10, 30, 50 | ≈60 | ||
Olea europaea L. (Olive) | Leaves | Ethanol: water (70:30 V/V) | Oleuropein | 10, 30, 90 | >100 | ||
Rosmarinus officinalis L. (Rosemary) | Leaves | Ethanol: water (50:50 V/V) | Rosmarinic acid | 10, 30, 90 | >100 | ||
Silbenes | Aloysia citrodora L. (Lemon verbena) | Leaves | Ethanol: water (60:40 V/V) | Verbascoside | 10, 30, 90 | >100 | |
Vitis Vinifera L. (Grape) | Root | Ethanol: water (50:50 V/V) | Resveratrol | 5, 10, 20 | ≈20 | ||
Diarylheptanoids | Curcuma longa L. (Tumeric) | Root | Ethanol | Curcuminoids | 2, 5, 10 | ≈8 | |
Flavonoids | Ginkgo biloba L. (Ginkgo) | Leaves | Ethanol: water (70:30 V/V) | Flavonoid glycosides | 10, 30, 90 | >100 | |
Glycine max. (L.) Merr (Soy) | Seed | Ethanol: water (50:50 V/V) | Isoflavone | 10, 30, 90 | >100 | ||
Vitis Vinifera L. (Grape) | Fruit | Water | Anthocyanins | 10, 30, 90 | >100 | ||
Camellia sinensis L. (Green tea) | Leaves | Ethanol: water (60:40 V/V) | Flavan-3-ols | 10, 30, 90 | >100 | ||
Vitis Vinifera L. (Grape) | Seed | Water | Proanthocyanins | 10, 30, 70 | ≈75 | ||
Citrus sp. (Orange) | Fruit | Ethanol: water (50:50 V/V) | Flavonoid glycosides (Hesperidin) | 10, 30, 90 | >100 | ||
Silybum marianum L. Gaertn. (Milk thistle) | Seed | Ethanol: water (70:30 V/V) | Silymarin | 10, 30, 90 | >100 |
Metabolic Process | Genes Implicated |
---|---|
Energetic metabolism, thermogenesis, and obesity | UCP2, HIF1A, PGC1a, FTO, MC4R, KCTD15, ETV5, NPY, |
Xenobiotic metabolism | AHR |
Lipid metabolism and adipogenesis | LEPR, LPL, PPARG, PPARA, PGC1a, AHR, LIPC, FTO, CD36 |
Cholesterol and lipoprotein homeostasis | LPL, PPARA, ABCA1, APOA5 |
Glucose homeostasis and diabetes | TCF7L2, PGC1a, TMEM18, GNPDA2, GCKR |
Blood pressure | AGT, ACE |
Mitochondrial biogenesis | PPARG, PGC1a |
Metabolic response to exercise and muscle capacity | MTHFR, PPARG, HFE, HIF1A, PGC1a |
Aerobic capacity and sport performance | ADRB1, ACE, HIF1A |
Structural function | COL5A1, |
Biorhythm | CLOCK |
Appetite | NPY, LEPR |
Immune response | IL4R, PPARA |
Senescence and aging-related diseases | MTHFR, ABCA1, BNC2, COL51A, DLGAP1, CD36, |
Cognitive function | DLGAP1, COMT, ETV5, NPY |
Food-related intolerances (gluten, lactose, fructose) | HLA-DQA1, MCM6 |
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Reguero, M.; Gómez de Cedrón, M.; Reglero, G.; Quintela, J.C.; Ramírez de Molina, A. Natural Extracts to Augment Energy Expenditure as a Complementary Approach to Tackle Obesity and Associated Metabolic Alterations. Biomolecules 2021, 11, 412. https://doi.org/10.3390/biom11030412
Reguero M, Gómez de Cedrón M, Reglero G, Quintela JC, Ramírez de Molina A. Natural Extracts to Augment Energy Expenditure as a Complementary Approach to Tackle Obesity and Associated Metabolic Alterations. Biomolecules. 2021; 11(3):412. https://doi.org/10.3390/biom11030412
Chicago/Turabian StyleReguero, Marina, Marta Gómez de Cedrón, Guillermo Reglero, José Carlos Quintela, and Ana Ramírez de Molina. 2021. "Natural Extracts to Augment Energy Expenditure as a Complementary Approach to Tackle Obesity and Associated Metabolic Alterations" Biomolecules 11, no. 3: 412. https://doi.org/10.3390/biom11030412