Double-Edged Sword Effect of Pyroptosis: The Role of Caspase-1/-4/-5/-11 in Different Levels of Apical Periodontitis
Abstract
:1. Introduction
2. Material and Methods
2.1. Clinical Specimens
2.2. Label-Free Proteomics
2.3. Establishment of the Experimental Apical Periodontitis (EAP) Rat Model
2.4. Micro-CT Analysis
2.5. Hematoxylin-Eosin Staining (HE) and Immunofluorescence
2.6. TUNEL Staining
2.7. Cell Culture
2.8. Lactic Dehydrogenase (LDH) Release Assay
2.9. Propidium Iodide (PI) Staining
2.10. Western Blot
2.11. Data Analysis
3. Results
3.1. Caspase-1 and Its Related Proteins Were Only Detected in Human Chronic AP
3.2. Caspase-1/-4/-5 Differentially Mediated Canonical and Noncanonical Pyroptosis in Human AP
3.3. Caspase-1/-11-Mediated Pyroptosis Contributed to the Progression of EAP
3.4. Establishment of a Dual EAP Model with Both Smaller (in Mandible) and Larger (in Maxilla) Bone Lesions
3.5. Pyroptosis Is a Double-Edged Sword in Inducing Bone Loss in Periapical Inflammation
3.6. LPS Induced Pyroptosis in THP-1-Derived Macrophages. Inhibition of Pyroptosis Showed a Double-Edged Sword Effect in the In Vitro Models
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Wu, Z.; Li, M.; Ren, X.; Zhang, R.; He, J.; Cheng, L.; Cheng, R.; Hu, T. Double-Edged Sword Effect of Pyroptosis: The Role of Caspase-1/-4/-5/-11 in Different Levels of Apical Periodontitis. Biomolecules 2022, 12, 1660. https://doi.org/10.3390/biom12111660
Wu Z, Li M, Ren X, Zhang R, He J, Cheng L, Cheng R, Hu T. Double-Edged Sword Effect of Pyroptosis: The Role of Caspase-1/-4/-5/-11 in Different Levels of Apical Periodontitis. Biomolecules. 2022; 12(11):1660. https://doi.org/10.3390/biom12111660
Chicago/Turabian StyleWu, Zhiwu, Mingming Li, Xiaolin Ren, Rui Zhang, Jinfeng He, Li Cheng, Ran Cheng, and Tao Hu. 2022. "Double-Edged Sword Effect of Pyroptosis: The Role of Caspase-1/-4/-5/-11 in Different Levels of Apical Periodontitis" Biomolecules 12, no. 11: 1660. https://doi.org/10.3390/biom12111660