Star Polymers as Non-Viral Carriers for Apoptosis Induction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Synthesis of Star Polymer with Poly(Arylene Oxindole) Core and Poly(N,N′-Dimethylaminoethyl Methacrylate) Arms
2.3. Cytotoxicity Assay
2.4. Fluorescence
2.5. Apoptosis Assay
2.6. Flow Cytometry
2.7. RNA Isolation and Quantitative RT-PCR
2.8. Statistical Analysis
3. Results
3.1. Toxicity of Star Polymer Is Concentration-Dependent and Induces Mainly Early Apoptosis in HT-1080 Cells
3.2. HT-1080 Cells Treatment with Star Polymer Triggers Changes of Gene Expression Profile and Activates Various Apoptotic Pathways
3.3. Star Polymer Causes the Early Apoptosis in Human Bladder Cancer Cell Lines
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Fus-Kujawa, A.; Sieroń, Ł.; Dobrzyńska, E.; Chajec, Ł.; Mendrek, B.; Jarosz, N.; Głowacki, Ł.; Dubaj, K.; Dubaj, W.; Kowalczuk, A.; et al. Star Polymers as Non-Viral Carriers for Apoptosis Induction. Biomolecules 2022, 12, 608. https://doi.org/10.3390/biom12050608
Fus-Kujawa A, Sieroń Ł, Dobrzyńska E, Chajec Ł, Mendrek B, Jarosz N, Głowacki Ł, Dubaj K, Dubaj W, Kowalczuk A, et al. Star Polymers as Non-Viral Carriers for Apoptosis Induction. Biomolecules. 2022; 12(5):608. https://doi.org/10.3390/biom12050608
Chicago/Turabian StyleFus-Kujawa, Agnieszka, Łukasz Sieroń, Estera Dobrzyńska, Łukasz Chajec, Barbara Mendrek, Natalia Jarosz, Łukasz Głowacki, Kamila Dubaj, Wojciech Dubaj, Agnieszka Kowalczuk, and et al. 2022. "Star Polymers as Non-Viral Carriers for Apoptosis Induction" Biomolecules 12, no. 5: 608. https://doi.org/10.3390/biom12050608