Spermine Oxidase–Substrate Electrostatic Interactions: The Modulation of Enzyme Function by Neighboring Colloidal ɣ-Fe2O3
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Instrumental Analysis
3. Results
3.1. Chemical–Physical Characterization of the SAMN@SMOX Hybrid
3.2. Comparison of the Activity of Native SMOX and of SAMN@SMOX Hybrid
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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2C·Zenz·Zsub | |||
---|---|---|---|
kcat | 1/KM | kcat/KM | |
Native SMOX | −5.6 | +7.7 | +1.9 |
SAMN@SMOX | −1.6 | −3.9 | −5.9 |
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Rilievo, G.; Magro, M.; Tonolo, F.; Cecconello, A.; Rutigliano, L.; Cencini, A.; Molinari, S.; Di Paolo, M.L.; Fiorucci, C.; Rossi, M.N.; et al. Spermine Oxidase–Substrate Electrostatic Interactions: The Modulation of Enzyme Function by Neighboring Colloidal ɣ-Fe2O3. Biomolecules 2023, 13, 1800. https://doi.org/10.3390/biom13121800
Rilievo G, Magro M, Tonolo F, Cecconello A, Rutigliano L, Cencini A, Molinari S, Di Paolo ML, Fiorucci C, Rossi MN, et al. Spermine Oxidase–Substrate Electrostatic Interactions: The Modulation of Enzyme Function by Neighboring Colloidal ɣ-Fe2O3. Biomolecules. 2023; 13(12):1800. https://doi.org/10.3390/biom13121800
Chicago/Turabian StyleRilievo, Graziano, Massimiliano Magro, Federica Tonolo, Alessandro Cecconello, Lavinia Rutigliano, Aura Cencini, Simone Molinari, Maria Luisa Di Paolo, Cristian Fiorucci, Marianna Nicoletta Rossi, and et al. 2023. "Spermine Oxidase–Substrate Electrostatic Interactions: The Modulation of Enzyme Function by Neighboring Colloidal ɣ-Fe2O3" Biomolecules 13, no. 12: 1800. https://doi.org/10.3390/biom13121800