Biomarker Signature in Aqueous Humor Mirrors Lens Epithelial Cell Activation: New Biomolecular Aspects from Cataractogenic Myopia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population: Clinical Assessment and Sampling
2.2. Pre-Analytical Procedures
2.3. Analytical Procedures
2.3.1. Ella™ and ELISA Analysis
2.3.2. Molecular Analysis: 2-Step Real-Time RT-PCR Analysis
2.3.3. Microscopy, Digital Imaging, and Densitometry
2.4. Statistical Analysis
3. Results
3.1. AH Expression of IL-6, IL-8, VEGF-A, and ANG-2
3.2. LECs Express the αSMA-Phenotype (Protein and Transcript) That Correlates with AL Elongation
3.3. LECs Express Transcripts Specific for TGFβR and TGFβ Isoforms
3.4. LECs might Contribute and Respond to AH Signature
3.5. IL-6R/IL-6 and VEGF-R1/VEGF-A Transcripts Correlate with α-SMA Transcripts
3.6. Correlation Analyses for AH Signature and Eye Elongation: Ocular Fluid Circulation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Study Population | Emmetropia | Myopia | High Myopia | p Value | |
---|---|---|---|---|---|
Subjects | 102 | 38 | 32 | 32 | p > 0.05 |
Age | 74.87 ± 9.47 | 78.22 ± 7.43 | 77.7 ± 7.80 | 67.71 ± 9.78 | p > 0.05 |
Sex F/M | 58/44 | 23/15 | 13/19 | 22/10 | p > 0.05 |
Axial Length | 24.98 ± 2.77 | 23.04 ± 0.51 | 24.92 ± 0.41 | 29.57 ± 0.66 | p < 0.05 |
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De Piano, M.; Cacciamani, A.; Balzamino, B.O.; Scarinci, F.; Cosimi, P.; Cafiero, C.; Ripandelli, G.; Micera, A. Biomarker Signature in Aqueous Humor Mirrors Lens Epithelial Cell Activation: New Biomolecular Aspects from Cataractogenic Myopia. Biomolecules 2023, 13, 1328. https://doi.org/10.3390/biom13091328
De Piano M, Cacciamani A, Balzamino BO, Scarinci F, Cosimi P, Cafiero C, Ripandelli G, Micera A. Biomarker Signature in Aqueous Humor Mirrors Lens Epithelial Cell Activation: New Biomolecular Aspects from Cataractogenic Myopia. Biomolecules. 2023; 13(9):1328. https://doi.org/10.3390/biom13091328
Chicago/Turabian StyleDe Piano, Maria, Andrea Cacciamani, Bijorn Omar Balzamino, Fabio Scarinci, Pamela Cosimi, Concetta Cafiero, Guido Ripandelli, and Alessandra Micera. 2023. "Biomarker Signature in Aqueous Humor Mirrors Lens Epithelial Cell Activation: New Biomolecular Aspects from Cataractogenic Myopia" Biomolecules 13, no. 9: 1328. https://doi.org/10.3390/biom13091328