Integrated Analysis of lncRNAs and mRNAs Reveals Complex Gene Network Mediated by lncRNAs and Regulatory Function of MuLRR-RLK-AS in Response to Phytoplasma Infection in Mulberry
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Growth Conditions
2.2. Construction of cDNA Libraries and High-Throughput Sequencing
2.3. Identification of lncRNAs and Target Prediction
2.4. Gene Expression Analysis and qRT-PCR Validation
2.5. Gene Ontology Analysis
2.6. Gene Cloning and Phylogenetic Analysis
2.7. Subcellular Localization
2.8. Promoter Activity Analysis
2.9. Production of Transgenic Arabidopsis
2.10. Plant Treatment
2.11. Detection of Colony-Forming Units
2.12. Statistic Analysis
3. Results
3.1. High-Throughput Sequencing and DEG Analysis
3.2. Identification and Characterization of lncRNAs
3.3. Target Prediction of the Differentially Expressed lncRNAs (DELs)
3.4. Characterization of MuLRR-RLK-AS Trans-Target Gene MuLRR-RLK
3.5. Expression Patterns of MuLRR-RLK and MuLRR-RLK-AS
3.6. MuLRR-RLK-AS Represses the Expression of MuLRR-RLK
3.7. MuLRR-RLK-AS Is a Negative Regulator of Plant Disease Resistance
3.8. Ectopic Expression of MuLRR-RLK Affects the Expression of Defense-Related Genes
4. Discussion
4.1. LncRNAs Are Involved in Regulating Gene Expression in Both Cis- and Trans-Manners in Response to Phytoplasma Infection
4.2. MuLRR-RLK-AS Plays an Important Role in Regulating MuLRR-RLK Gene Expression in Response to Phytoplasma Infection
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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LncRNAs | Up/Down Stream | Co-Localization Differentially Expressed Target Genes | |||||||
---|---|---|---|---|---|---|---|---|---|
ID | log2FoldChange (IL/HL) | p Value | Up/Down | ID | log2FoldChange (IL/HL) | p Value | Up/Down | Description | |
XLOC_000009 | −1.659904691 | 2.01 × 10−5 | Down | Upstream_2k | XP_010086539.1 | −6.285402219 | 9.71 × 10−11 | Down | Uncharacterized protein LOC21384651 |
XLOC_003582 | −1.357748564 | 4.19 × 10−63 | Down | Downstream_2k | XP_010089960.1 | 1.622764533 | 2.69 × 10−30 | Up | NADP-dependent glyceraldehyde-3-phosphate dehydrogenase |
XLOC_004104 | 2.30176161 | 0 | Up | Upstream_2k | XP_010090441.1 | −1.498805857 | 8.26 × 10−9 | Down | NAC domain containing protein 50 isoform X1 |
XLOC_004314 | −11.62763875 | 4.50 × 10−161 | Down | Downstream_2k | XP_010090692.1 | 5.044394119 | 5.83 × 10−8 | Up | Uncharacterized protein |
XLOC_005352 | −1.359639818 | 7.41 × 10−74 | Down | Downstream_2k | XP_010091710.1 | −1.75161237 | 0.00017364 | Down | Hypothetical protein |
XLOC_005353 | −2.142379817 | 1.19 × 10−54 | Down | Downstream_2k | XP_010091710.1 | −1.75161237 | 0.00017364 | Down | Hypothetical protein |
XLOC_006216 | −8.087982542 | 0.000355214 | Down | Downstream_2k | XP_010092498.1 | 11.06173466 | 0 | Up | Hypothetical protein |
XLOC_006461 | −2.220725888 | 3.24 × 10−11 | Down | Upstream_2k | XP_010092702.1 | −2.115477217 | 8.81 × 10−8 | Down | Pentatricopeptide repeat-containing protein |
XLOC_006690 | −1.496801279 | 2.07 × 10−6 | Down | Downstream_2k | XP_010092905.1 | 1.325507985 | 2.24 × 10−35 | Up | Common plant regulatory factor 1 |
XLOC_006961 | −1.32577553 | 4.79 × 10−5 | Down | Upstream_2k | XP_010093164.1 | −2.191426071 | 1.03 × 10−19 | Down | Hypothetical protein |
XLOC_007096 | −1.048521438 | 2.67 × 10−8 | Down | Downstream_2k | XP_010093303.1 | −1.415037499 | 1.18 × 10−5 | Down | ATP-dependent zinc metalloprotease FtsH |
XLOC_008373 | 2.902444746 | 5.16 × 10−6 | Up | Upstream_2k | XP_010094597.1 | 1.378394431 | 4.77 × 10−91 | Up | 4-coumarate--CoA ligase-like 5 |
XLOC_008398 | −2.45656083 | 0.474176 | Down | Upstream_2k | XP_010094597.1 | 1.378394431 | 4.77 × 10−91 | Up | 4-coumarate--CoA ligase-like 5 |
XLOC_016866 | −1.108741537 | 3.09 × 10−6 | Down | Downstream_2k | XP_010094598.1 | −1.708344916 | 7.10 × 10−19 | Down | Protein ROOT PRIMORDIUM DEFECTIVE 1 |
XLOC_009663 | −2.043818661 | 1.86 × 10−23 | Down | Upstream_2k | XP_010095745.1 | −2.172404286 | 9.14 × 10−119 | Down | Pyruvate kinase isozyme A |
XLOC_011123 | −1.699511675 | 1.81 × 10−14 | Down | Downstream_2k | XP_010097263.1 | −1.570240995 | 7.69 × 10−21 | Down | Hypothetical protein |
XLOC_011929 | 1.009110152 | 2.46 × 10−50 | Up | Downstream_2k | XP_010097937.1 | −1.653442239 | 6.36 × 10−61 | Down | Retrovirus-related Pol polyprotein from transposon TNT 1–94 |
XLOC_011968 | −3.450165156 | 0.000183881 | Down | Downstream_2k | XP_010097994.1 | −6.087462841 | 0.00017296 | Down | GDT1-like protein 2 |
XLOC_012270 | −1.271836651 | 1.07 × 10−23 | Down | Upstream_2k | XP_010098256.1 | −1.115122616 | 1.46 × 10−36 | Down | DEAD-box ATP-dependent RNA helicase ISE2 |
XLOC_012337 | −2.438980184 | 0.474176 | Down | Upstream_2k | XP_010098340.1 | −2.850856561 | 0.00017039 | Down | Zinc finger CCCH domain-containing protein 58 |
XLOC_012366 | −2.450477186 | 0.0266538 | Down | Upstream_2k | XP_010098340.1 | −2.850856561 | 0.00017039 | Down | Zinc finger CCCH domain-containing protein 58 |
XLOC_014320 | −2.086989256 | 5.76 × 10−12 | Down | Downstream_2k | XP_010100223.1 | −1.047305715 | 1.35 × 10−7 | Down | Ubiquitin carboxyl-terminal hydrolase 10 |
XLOC_014820 | −1.871361676 | 6.68 × 10−98 | Down | Downstream_2k | XP_010100733.1 | −4.037089319 | 0.00018388 | Down | Hypothetical protein |
XLOC_015257 | −2.853283109 | 0.474176 | Down | Upstream_2k | XP_010101095.1 | −1.461049897 | 2.36 × 10−7 | Down | Histone-lysine N-methyltransferase |
XLOC_015724 | 12.61184784 | 4.13 × 10−69 | Up | Downstream_2k | XP_010101540.1 | −2.722049907 | 0 | Down | GDSL esterase/lipase |
XLOC_017908 | −1.9200952 | 1.66 × 10−142 | Down | Upstream_2k | XP_010103622.1 | −1.972293071 | 6.04 × 10−21 | Down | Dihydroorotate dehydrogenase (quinone) |
XLOC_018202 | −1.743622263 | 5.76 × 10−133 | Down | Upstream_2k | XP_010103913.1 | −1.545434137 | 3.59 × 10−9 | Down | Hypothetical protein |
XLOC_018332 | 2.51571606 | 0.000105589 | Up | Downstream_2k | XP_010104046.1 | −1.906890596 | 9.50 × 10−8 | Down | Hypothetical protein |
XLOC_018333 | 1.508625478 | 2.35 × 10−15 | Up | Downstream_2k | XP_010104046.1 | −1.906890596 | 9.50 × 10−8 | Down | Hypothetical protein |
XLOC_018334 | 1.136596235 | 1.14 × 10−12 | Up | Downstream_2k | XP_010104046.1 | −1.906890596 | 9.50 × 10−8 | Down | Hypothetical protein |
XLOC_018352 | 1.274845329 | 8.73 × 10−16 | Up | Upstream_2k | XP_010104047.1 | −1.465663572 | 4.78 × 10−11 | Down | Glucuronoxylan 4-O-methyltransferase 1 |
XLOC_018600 | 1.232238957 | 0.000128139 | Up | Downstream_2k | XP_010104288.1 | −1.080959321 | 2.26 × 10−9 | Down | Uncharacterized protein LOC21388859 |
XLOC_018675 | −1.476521201 | 4.78 × 10−16 | Down | Upstream_2k | XP_010104373.1 | −1.862496476 | 0.00022881 | Down | Two-component response regulator-like protein |
XLOC_018680 | −2.179955771 | 2.14 × 10−6 | Down | Upstream_2k | XP_010104373.1 | −1.862496476 | 0.00022881 | Down | Two-component response regulator-like protein |
XLOC_018692 | −2.139247136 | 2.33 × 10−37 | Down | Upstream_2k | XP_010104373.1 | −1.862496476 | 0.00022881 | Down | Two-component response regulator-like protein |
XLOC_018696 | 12.2276009 | 3.01 × 10−28 | Up | Upstream_2k | XP_010104373.1 | −1.862496476 | 0.00022881 | Down | Two-component response regulator-like protein |
XLOC_019166 | −1.120989021 | 2.10 × 10−94 | Down | Downstream_2k | XP_010104915.1 | −1.100273908 | 1.37 × 10−28 | Down | Uncharacterized protein LOC21406865 |
XLOC_019555 | −2.58203706 | 0.0595904 | Down | Downstream_2k | XP_010105201.1 | −5.087462841 | 2.00 × 10−5 | Down | Sn1-specific diacylglycerol lipase beta |
XLOC_019810 | 14.10733152 | 1.15 × 10−47 | Up | Downstream_2k | XP_010105413.1 | −1.70571466 | 2.40 × 10−31 | Down | Putative GDP-L-fucose synthase 2 |
XLOC_020110 | −1.030050297 | 2.09 × 10−24 | Down | Upstream_2k | XP_010105705.1 | −1.650322233 | 8.17 × 10−45 | Down | Guanylate kinase 2 |
XLOC_020112 | 1.243959167 | 7.39 × 10−25 | Up | Upstream_2k | XP_010105705.1 | −1.650322233 | 8.17 × 10−45 | Down | Guanylate kinase 2 |
XLOC_020267 | −1.009344657 | 8.55 × 10−6 | Down | Downstream_2k | XP_010105833.1 | −2.321928095 | 1.57 × 10−10 | Down | Pre-mRNA-processing protein 40A isoform X2 |
XLOC_020549 | −3.468308658 | 2.93 × 10−10 | Down | Upstream_2k | XP_010106108.1 | −7.6794801 | 8.38 × 10−20 | Down | Hypothetical protein |
XLOC_020799 | −3.174801049 | 8.27 × 10−9 | Down | Upstream_2k | XP_010106332.1 | 4.558967292 | 1.88 × 10−156 | Up | Hypothetical protein |
XLOC_020823 | −1.290572462 | 9.68 × 10−10 | Down | Upstream_2k | XP_010106349.1 | −3.019590728 | 1.52 × 10−41 | Down | Hypothetical protein |
XLOC_020815 | −1.550204751 | 4.40 × 10−5 | Down | Downstream_2k | XP_010106354.1 | 2.674712213 | 1.36 × 10−243 | Up | G-type lectin S-receptor-like serine/threonine-protein kinase |
XLOC_021165 | −1.220913717 | 3.65 × 10−8 | Down | Upstream_2k | XP_010106679.1 | 1.85708704 | 4.53 × 10−17 | Up | Inorganic pyrophosphatase 1 |
XLOC_021166 | −2.999715655 | 2.63 × 10−30 | Down | Upstream_2k | XP_010106679.1 | 1.85708704 | 4.53 × 10−17 | Up | Inorganic pyrophosphatase 1 |
XLOC_021967 | −1.459141927 | 3.89 × 10−9 | Down | Downstream_2k | XP_010107456.1 | −3.378511623 | 2.13 × 10−13 | Down | uncharacterized protein LOC21385704 isoform X1 |
XLOC_022178 | −1.074733339 | 1.69 × 10−54 | Down | Downstream_2k | XP_010107670.1 | 1.48112669 | 2.62 × 10−11 | Up | probable indole-3-acetic acid-amido synthetase GH3.6 |
XLOC_023091 | 2.645694109 | 1.02 × 10−6 | Up | Downstream_2k | XP_010108540.1 | −2.264499815 | 3.96 × 10−15 | Down | hypothetical protein |
XLOC_024046 | −2.458590043 | 0.230884 | Down | Downstream_2k | XP_010109450.1 | −1.638260727 | 1.48 × 10−60 | Down | mitochondrial carrier protein MTM1 isoform X1 |
XLOC_024072 | −1.363720387 | 1.03 × 10−15 | Down | Downstream_2k | XP_010109466.1 | −1.469695084 | 8.90 × 10−40 | Down | hypothetical protein |
XLOC_024250 | 1.511943985 | 4.91 × 10−15 | Up | Downstream_2k | XP_010109645.1 | 1.030214613 | 9.23 × 10−51 | Up | transcription factor DIVARICATA |
XLOC_024279 | −2.968724068 | 0.474176 | Down | Upstream_2k | XP_010109661.1 | −2.164282855 | 1.62 × 10−187 | Down | pentatricopeptide repeat-containing protein |
XLOC_024407 | −1.218449856 | 1.04 × 10−22 | Down | Upstream_2k | XP_010109786.1 | −1.513069582 | 3.18 × 10−11 | Down | uncharacterized protein At4g10930 |
XLOC_025437 | −2.561579471 | 0.474176 | Down | Downstream_2k | XP_010110803.1 | 1.12963528 | 2.27 × 10−5 | Up | RING finger protein B |
XLOC_025452 | −2.474769468 | 0.474176 | Down | Upstream_2k | XP_010110804.1 | 5.720122084 | 8.40 × 10−129 | Up | leucoanthocyanidin reductase |
XLOC_025453 | 3.77129058 | 6.73 × 10−9 | Up | Upstream_2k | XP_010110804.1 | 5.720122084 | 8.40 × 10−129 | Up | leucoanthocyanidin reductase |
XLOC_025463 | 1.448311774 | 5.88 × 10−9 | Up | Upstream_2k | XP_010110804.1 | 5.720122084 | 8.40 × 10−129 | Up | leucoanthocyanidin reductase |
XLOC_025605 | 1.268119766 | 1.27 × 10−140 | Up | Downstream_2k | XP_010110915.1 | −1.776621795 | 1.26 × 10−42 | Down | hypothetical protein |
XLOC_025845 | 1.086054103 | 0.000228706 | Up | Upstream_2k | XP_010111144.1 | −5.584962501 | 2.31 × 10−6 | Down | hypothetical protein |
XLOC_025906 | 1.313631149 | 7.94 × 10−25 | Up | Upstream_2k | XP_010111198.1 | −1.252187024 | 3.51 × 10−8 | Down | putative E3 ubiquitin-protein ligase RING1a isoform X2 |
XLOC_025907 | 2.290116081 | 5.36 × 10−23 | Up | Upstream_2k | XP_010111198.1 | −1.252187024 | 3.51 × 10−8 | Down | putative E3 ubiquitin-protein ligase RING1a isoform X2 |
XLOC_025909 | −1.440184589 | 9.82 × 10−6 | Up | Upstream_2k | XP_010111198.1 | −1.252187024 | 3.51 × 10−8 | Down | putative E3 ubiquitin-protein ligase RING1a isoform X2 |
XLOC_026031 | −1.440184589 | 9.82 × 10−6 | Down | Downstream_2k | XP_010111442.1 | 1.015266757 | 0.0001083 | Up | hypothetical protein |
XLOC_026652 | −2.355017768 | 0.474176 | Down | Upstream_2k | XP_010111922.1 | −2.30256277 | 1.38 × 10−15 | Down | uncharacterized protein LOC21393124 |
XLOC_026790 | −1.123960181 | 9.54 × 10−5 | Down | Upstream_2k | XP_010112073.1 | −2.692490965 | 1.52 × 10−20 | Down | ATP-dependent Clp protease ATP-binding subunit ClpX |
LncRNAs | Target Genes | |||||||
---|---|---|---|---|---|---|---|---|
ID | log2FoldChange (B/J) | p Value | Up/Down | ID | log2FoldChange (BB/JJ) | p Value | Up/Down | Descrition |
XLOC_001590 | 3.837441902 | 0.000471504 | Up | XP_010088049.1 | 2.605901838 | 6.15 × 10−43 | Up | Chaperone protein DnaJ |
XLOC_003017 | 4.009664269 | 0.012449373 | Up | XP_010089382.1 | −1.15417093 | 2.37 × 10−8 | Down | PAP-specific phosphatase HAL2-like protein |
XLOC_003046 | −2.433070931 | 0.014546012 | Down | XP_010089404.1 | −2.06667104 | 3.31 × 10−9 | Down | Hypothetical protein L484_013795 |
XLOC_003503 | −1.599653771 | 1.22743 × 10−11 | Down | XP_010089852.1 | −7.033423002 | 2.00 × 10−5 | Down | Subtilisin-like protease |
XLOC_004551 | −1.779844507 | 0.034037713 | Down | XP_010090841.1 | −1.385653692 | 4.59 × 10−6 | Down | Putative polygalacturonase |
XLOC_004954 | 2.379663284 | 7.51328 × 10-−5 | Up | XP_010091231.1 | 2.011383996 | 0 | Up | Hypothetical protein L484_005255 ] |
XLOC_005131 | −2.31198078 | 0.003175704 | Down | XP_010091411.1 | −2.021479727 | 7.08 × 10−11 | Down | Chlorophyll a-b binding protein 16 |
XLOC_005342 | −8.732455766 | 2.79104 × 10−56 | Down | XP_010091645.1 | 2.479780264 | 5.94 × 10−26 | Up | UDP-glycosyltransferase 89A2 |
XLOC_006046 | −1.595761698 | 2.7063 × 10−13 | Down | XP_010092313.1 | −2.662965013 | 1.18 × 10−40 | Down | Putative galacturonosyltransferase 12 |
XLOC_006462 | −2.022357412 | 4.62933 × 10−36 | Down | XP_010092676.1 | −1.69538649 | 2.14 × 10−20 | Down | Hypothetical protein L484_019750 |
XLOC_009619 | −1.020506348 | 6.48115 × 10−7 | Down | XP_010095699.1 | −1.156119202 | 2.20 × 10−7 | Down | Putative endo-1,4-beta-xylanase C |
XLOC_010285 | −1.479609008 | 0.00017518 | Down | XP_010096371.1 | 5.169925001 | 0.00017525 | Up | Myb-related protein B |
XLOC_011021 | −2.356597415 | 1.18979 × 10−10 | Down | XP_010097040.1 | −2.938599455 | 1.17 × 10−8 | Down | Hypothetical protein L484_003871 |
XLOC_011087 | −1.189537055 | 1.92884 × 10−8 | Down | XP_010097097.1 | −1.310381065 | 0 | Down | Hypothetical protein L484_019536 |
XLOC_011132 | −1.325768866 | 3.97956 × 10−11 | Down | XP_010097155.1 | −1.830074999 | 7.78 × 10−6 | Down | ATPase 9, plasma membrane-type |
XLOC_011724 | −1.892272481 | 7.40323 × 10−13 | Down | XP_010097661.1 | −1.523561956 | 4.05 × 10−14 | Down | Phytosulfokine receptor 2 |
XLOC_012537 | −1.437284073 | 0.002462778 | Down | XP_010098515.1 | −1.659924558 | 4.93 × 10−27 | Down | Hypothetical protein L484_025954 |
XLOC_012821 | −1.545482034 | 1.99649 × 10−14 | Down | XP_010098738.1 | −1.74723393 | 2.02 × 10−8 | Down | Pectinesterase 3 |
XLOC_012923 | 1.151448857 | 5.66295 × 10−6 | Up | XP_010098833.1 | −1.27364808 | 3.01 × 10−6 | Down | UDP-glycosyltransferase 73C3 |
XLOC_013301 | −1.135788866 | 0.000554496 | Down | XP_010099215.1 | −1.963474124 | 3.86 × 10−34 | Down | Hypothetical protein L484_010155 |
XLOC_013697 | −2.587472054 | 9.67889 × 10−17 | Down | XP_010099597.1 | −1.17290886 | 5.38 × 10−6 | Down | Histone H3-like centromeric protein |
XLOC_015378 | −1.534366505 | 0.000274527 | Down | XP_010101197.1 | −1.021061616 | 0.000108539 | Down | Hypothetical protein L484_015001 |
XLOC_016174 | −1.659968292 | 5.73067 × 10−20 | Down | XP_010101961.1 | −2.408084739 | 2.01 × 10−5 | Down | Hypothetical protein L484_011978 |
XLOC_016448 | 9.017824501 | 0.008623184 | Up | XP_010102287.1 | 5.984893108 | 1.89 × 10−19 | Up | Hypothetical protein L484_024569 |
XLOC_017317 | −2.24788776 | 1.18979 × 10−10 | Down | XP_010103054.1 | −2.815174427 | 7.64 × 10−92 | Down | Hypothetical protein L484_001885 |
XLOC_017564 | −1.780652222 | 5.73067 × 10−20 | Down | XP_010103289.1 | 4.930737338 | 3.35 × 10−92 | Up | 3’-hydroxy-N-methyl-(S)-coclaurine 4’-O-methyltransferase |
XLOC_018767 | −2.14733932 | 8.58013 × 10−35 | Down | XP_010104430.1 | −4.087462841 | 0.000183881 | Down | Hypothetical protein L484_016029 |
XLOC_019411 | −1.597933878 | 0.006303773 | Down | XP_010105051.1 | −2.297266041 | 3.97 × 10−15 | Down | Hypothetical protein L484_001492 |
XLOC_020739 | −2.772398916 | 2.36945 × 10−8 | Down | XP_010106301.1 | −3.795859283 | 3.06 × 10−10 | Down | Magnesium-transporting ATPase, P-type 1 |
XLOC_020789 | 1.043080361 | 4.3553 × 10−7 | Up | XP_010106332.1 | 4.558967292 | 1.88 × 10−156 | Up | Hypothetical protein L484_004731 |
XLOC_021217 | −1.307207494 | 8.68908 × 10−7 | Down | XP_010106725.1 | −1.634715536 | 5.88 × 10−5 | Down | Myb-related protein Myb4 |
XLOC_021596 | −2.443238328 | 0.003175704 | Down | XP_010107118.1 | −1.093574115 | 9.06 × 10−5 | Down | NAC domain-containing protein 1 |
XLOC_022461 | −1.911411449 | 0.031545424 | Down | XP_010107850.1 | −2.275735285 | 7.51 × 10−53 | Down | Hypothetical protein L484_027437 |
XLOC_022591 | 2.284500804 | 8.77802 × 10−9 | Up | XP_010108041.1 | 1.83518913 | 1.25 × 10−6 | Up | Exocyst complex component 7 |
XLOC_022840 | 1.471119577 | 0.008783891 | Up | XP_010108284.1 | −6.906890596 | 3.53 × 10−19 | Down | Hypothetical protein L484_007137 |
XLOC_022990 | −9.988102379 | 7.94406 × 10−5 | Down | XP_010108424.1 | 2.03562391 | 8.43 × 10−11 | Up | Nudix hydrolase 15 |
XLOC_025085 | 9.747083561 | 6.51127 × 10−23 | Up | XP_010110394.1 | 1.094718657 | 0.000123589 | Up | Hypothetical protein L484_022797 |
XLOC_025632 | −2.675688053 | 7.45831 × 10−12 | Down | XP_010110923.1 | −1.80937409 | 2.02 × 10−73 | Down | Omega-hydroxypalmitate O-feruloyl transferase |
XLOC_026011 | −2.847603572 | 7.57567 × 10−8 | Down | XP_010111400.1 | 1.961110987 | 1.28 × 10−25 | Up | Multiple C2 and transmembrane domain-containing protein 2 |
XLOC_026715 | −2.99257368 | 2.63073 × 10−53 | Down | XP_010111977.1 | −3.89077093 | 2.45 × 10−17 | Down | Putative peptide/nitrate transporter |
XLOC_026802 | −2.726806211 | 4.61238 × 10−40 | Down | XP_010112045.1 | −1.807354922 | 1.62 × 10−8 | Down | Endoglucanase 9 |
XLOC_027017 | −1.271269054 | 5.03723 × 10−10 | Down | XP_010112258.1 | −1.332575339 | 0.000230944 | Down | MADS-box transcription factor 27 |
XLOC_027018 | −2.185948672 | 1.18979 × 10−10 | Down | XP_010112258.1 | −1.332575339 | 0.000230944 | Down | MADS-box transcription factor 27 |
XLOC_027445 | −1.081848858 | 0.033465549 | Down | XP_010112683.1 | 4.024384159 | 5.29 × 10−30 | Up | Putative LRR receptor-like serine/threonine-protein kinase |
Site Name | Number | Sequence | Function |
---|---|---|---|
ABRE | 2 | ACGTG | Cis-acting element involved in abscisic acid responsiveness |
ACE | 1 | GACACGTATG | Cis-acting element involved in light responsiveness |
ARE | 1 | AAACCA | Cis-acting regulatory element essential for anaerobic induction |
ATCT-motif | 1 | AATCTAATCC | Part of a conserved DNA module involved in light responsiveness |
AuxRR-core | 1 | GGTCCAT | Cis-acting regulatory element involved in auxin responsiveness |
Box 4 | 1 | ATTAAT | Part of a conserved DNA module involved in light responsiveness |
CAAT-box | 31 | CCAAT | Common cis-acting element in promoter and enhancer regions |
CAT-box | 1 | GCCACT | Cis-acting regulatory element related to meristem expression |
GARE-motif | 1 | TCTGTTG | Gibberellin-responsive element |
GATA-motif | 1 | GATAGGA | Part of a light responsive element |
G-Box | 3 | TACGTG | Cis-acting regulatory element involved in light responsiveness |
MBS | 1 | CGGTCA | MYB binding site involved in drought-inducibility |
P-box | 1 | CCTTTTG | Gibberellin-responsive element |
TATA-box | 13 | TATA | Core promoter element of approximately −30 transcription starts |
TATC-box | 1 | TATCCCA | Cis-acting element involved in gibberellin-responsiveness |
TCA-element | 1 | CCATCTTTTT | Cis-acting element involved in salicylic acid responsiveness |
TCT-motif | 1 | TCTTAC | Part of a light responsive element |
TGA-element | 1 | AACGAC | Auxin-responsive element |
Site Name | Number | Sequence | Function |
---|---|---|---|
ABRE | 2 | ACGTG | Cis-acting element involved in abscisic acid responsiveness |
ARE | 2 | AAACCA | Cis-acting regulatory element essential for anaerobic induction |
Box 4 | 1 | ATTAAT | Part of a conserved DNA module involved in light responsiveness |
CAAT-box | 46 | CCAAT | Common cis-acting element in promoter and enhancer regions |
CAT-box | 1 | GCCACT | Cis-acting regulatory element related to meristem expression |
CGTCA-motif | 2 | CGTCA | Cis-acting regulatory element involved in meja-responsiveness |
G-Box | 3 | TACGTG | Cis-acting regulatory element involved in light responsiveness |
GCN4_motif | 1 | TGAGTCA | Cis-regulatory element involved in endosperm expression |
GT1-motif | 1 | GGTTAA | Light responsive element |
LTR | 1 | CCGAAA | Cis-acting element involved in low-temperature responsiveness |
MBS | 1 | CGGTCA | MYB binding site involved in drought-inducibility |
P-box | 1 | CCTTTTG | Gibberellin-responsive element |
O2-site | 2 | GATGA(C/T)(A/G)TG(A/G) | Cis-acting regulatory element involved in zein metabolism regulation |
TATA-box | 46 | TATA | Core promoter element of approximately −30 transcription starts |
TC-rich repeats | 1 | GTTTTCTTAC | Cis-acting element involved in defense and stress responsiveness |
TCA-element | 1 | CCATCTTTTT | Cis-acting element involved in salicylic acid responsiveness |
TCT-motif | 1 | TCTTAC | Part of a light responsive element |
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Liu, Z.; Liu, C.; Zhao, T.; Yang, L.; Shang, Q.; Wang, G.; Liu, Z.; Gai, Y.; Ji, X. Integrated Analysis of lncRNAs and mRNAs Reveals Complex Gene Network Mediated by lncRNAs and Regulatory Function of MuLRR-RLK-AS in Response to Phytoplasma Infection in Mulberry. Biomolecules 2024, 14, 308. https://doi.org/10.3390/biom14030308
Liu Z, Liu C, Zhao T, Yang L, Shang Q, Wang G, Liu Z, Gai Y, Ji X. Integrated Analysis of lncRNAs and mRNAs Reveals Complex Gene Network Mediated by lncRNAs and Regulatory Function of MuLRR-RLK-AS in Response to Phytoplasma Infection in Mulberry. Biomolecules. 2024; 14(3):308. https://doi.org/10.3390/biom14030308
Chicago/Turabian StyleLiu, Zixuan, Chaorui Liu, Teng Zhao, Lulu Yang, Qiqi Shang, Gefan Wang, Zhaoyang Liu, Yingping Gai, and Xianling Ji. 2024. "Integrated Analysis of lncRNAs and mRNAs Reveals Complex Gene Network Mediated by lncRNAs and Regulatory Function of MuLRR-RLK-AS in Response to Phytoplasma Infection in Mulberry" Biomolecules 14, no. 3: 308. https://doi.org/10.3390/biom14030308