Extracellular Vesicles Induce Nuclear Factor-κB Activation and Interleukin-8 Synthesis through miRNA-191-5p Contributing to Inflammatory Processes: Potential Implications in the Pathogenesis of Chronic Obstructive Pulmonary Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. Cell Culture
2.3. Cell Transfection with miR-191-5p Mimic and miR-191-5p Inhibitor
2.4. Evaluation of miR-191-5p Expression
2.5. Elisa for IL-6 and IL-8 Detection
2.6. NF-kB Activity Evaluation
2.7. Study Cohort and Functional Tests
2.8. Isolation and Characterization of Extracellular Vesicles
2.8.1. From Plasma of Patients
2.8.2. From Cell Medium
2.9. Network Analysis and Molecules Activity Prediction
2.10. Statistical Analysis
3. Results
3.1. miR-191-5p-Enriched EV Increased miR-191-5p in Recipient Cells
3.2. miR-191-5p-Enriched EVs Activate the Inflammatory Pathway
3.3. Correlation of EV-Derived miR-191-5p with Clinical Parameters
3.4. Bioinformatic Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Age, years | 71.0 (6.0) |
Birth sex, female:male, n | 9:26 |
Smoking history | |
| 9 |
| 26 |
Pack-years | 49.5 ± 23.4 |
Dyspnea, mMRC | 1 (1) |
BMI, Kg/m2 | 29.6 ± 6.1 |
FEV1, L | 1.34 ± 0.44 |
% pred. | 53.9 ± 11.8 |
FEV1/FVC, % | 48.0 ± 11.3 |
TLC, L | 6.70 ± 1.49 |
% pred. | 112.0 ± 19.3 |
DLCO, mL/min/mmHg | 17.1 ± 5.5 |
% pred. | 72.8 ± 22.5 |
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Carpi, S.; Polini, B.; Nieri, D.; Doccini, S.; Conti, M.; Bazzan, E.; Pagnini, M.; Santorelli, F.M.; Cecchini, M.; Nieri, P.; et al. Extracellular Vesicles Induce Nuclear Factor-κB Activation and Interleukin-8 Synthesis through miRNA-191-5p Contributing to Inflammatory Processes: Potential Implications in the Pathogenesis of Chronic Obstructive Pulmonary Disease. Biomolecules 2024, 14, 1030. https://doi.org/10.3390/biom14081030
Carpi S, Polini B, Nieri D, Doccini S, Conti M, Bazzan E, Pagnini M, Santorelli FM, Cecchini M, Nieri P, et al. Extracellular Vesicles Induce Nuclear Factor-κB Activation and Interleukin-8 Synthesis through miRNA-191-5p Contributing to Inflammatory Processes: Potential Implications in the Pathogenesis of Chronic Obstructive Pulmonary Disease. Biomolecules. 2024; 14(8):1030. https://doi.org/10.3390/biom14081030
Chicago/Turabian StyleCarpi, Sara, Beatrice Polini, Dario Nieri, Stefano Doccini, Maria Conti, Erika Bazzan, Marta Pagnini, Filippo Maria Santorelli, Marco Cecchini, Paola Nieri, and et al. 2024. "Extracellular Vesicles Induce Nuclear Factor-κB Activation and Interleukin-8 Synthesis through miRNA-191-5p Contributing to Inflammatory Processes: Potential Implications in the Pathogenesis of Chronic Obstructive Pulmonary Disease" Biomolecules 14, no. 8: 1030. https://doi.org/10.3390/biom14081030