Investigating the Impact of Estrogen Levels on Voiding Characteristics, Bladder Structure, and Related Proteins in a Mouse Model of Menopause-Induced Lower Urinary Tract Symptoms
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Design of Animal Model
2.2. Evaluation of Estradiol Level
2.3. Statistics on the Frequency of Urination
2.4. Bladder Cystometry (Urodynamics)
2.5. Masson Staining
2.6. Immunofluorescence Staining
2.7. Quantitative Real-Time PCR Assay
2.8. Statistical Analysis
3. Results
3.1. Establishment and Validation of Animal Models
3.2. Urination Frequency in Mice Correlates with Estrogen Levels
3.3. Bladder Function Is Correlated with Estrogen Levels in Mice
3.4. Estrogen Intervention Decreases Collagen Deposition in Bladder Tissue
3.5. Estrogen Intervention Decreases Transcript Levels of Piezo1 and TRPV4 in Mice Bladder Tissue
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Forward Primer (5′→3′) | Forward Primer (5′→3′) |
---|---|---|
Piezo1 | ATCGCCATCATCTGGTTCCC | AGCTCCAAGGTGTGCTTCTC |
TRPV4 | TGAGCAGGCCGAGAAGTACA | AGTCCATCTAGGTCCGCAGT |
Collagen I | ACGTAAGCACTGGTGGACAG | CAGGAGGGCCATAGCTGAAC |
Collagen III | GAGGAATGGGTGGCTATCCG | TTGCGTCCATCAAAGCCTCT |
β-actin | GCAGGAGTACGATGAGTCCG | AGTCCATCTAGGTCCGCAGT |
Variable | SHAM | OVX | E2 |
---|---|---|---|
Serum estradiol level (pg/mL) | 35.20 ± 17.21 | 1.80 ± 1.32 **** | 38.18 ± 22.00 |
Water intake (mL/48 h) | 9.50 ± 1.38 | 10.69 ± 3.42 | 8.00 ± 1.26 |
Body weight (g) | 17.59 ± 0.58 | 20.22 ± 1.17 *** | 19.13 ± 1.99 |
Bladder weight (mg) | 17.00 ± 1.60 | 18.00 ± 2.60 | 25.00 ± 2.20 ** |
The ratio of bladder weight (mg)/body weight (g) | 0.89 ± 0.13 | 0.90 ± 0.15 | 1.1 ± 0.14 |
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Zhang, C.; Chen, Y.; Yin, L.; Deng, G.; Xia, X.; Tang, X.; Zhang, Y.; Yan, J. Investigating the Impact of Estrogen Levels on Voiding Characteristics, Bladder Structure, and Related Proteins in a Mouse Model of Menopause-Induced Lower Urinary Tract Symptoms. Biomolecules 2024, 14, 1044. https://doi.org/10.3390/biom14091044
Zhang C, Chen Y, Yin L, Deng G, Xia X, Tang X, Zhang Y, Yan J. Investigating the Impact of Estrogen Levels on Voiding Characteristics, Bladder Structure, and Related Proteins in a Mouse Model of Menopause-Induced Lower Urinary Tract Symptoms. Biomolecules. 2024; 14(9):1044. https://doi.org/10.3390/biom14091044
Chicago/Turabian StyleZhang, Chenglong, Yuangui Chen, Lingxuan Yin, Guoxian Deng, Xiaowen Xia, Xiaoshuang Tang, Yifeng Zhang, and Junan Yan. 2024. "Investigating the Impact of Estrogen Levels on Voiding Characteristics, Bladder Structure, and Related Proteins in a Mouse Model of Menopause-Induced Lower Urinary Tract Symptoms" Biomolecules 14, no. 9: 1044. https://doi.org/10.3390/biom14091044