Numb Suppresses Notch-Dependent Activation of Enhancer of split during Lateral Inhibition in the Drosophila Embryonic Nervous System
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fly Stocks
2.2. Preparation of Fly Food
2.3. Generation of numb Maternal and Zygotic Mutant Embryos
2.4. Immunohistochemical Staining
2.5. Scoring the Neurogenic and Antineurogenic Phenotypes of numb Mutants
2.6. Confocal Microscope
3. Results
3.1. Strong Loss-of-Function Mutations in numb Universally Induce an Antineurogenic Phenotype in the PNS of Embryos
3.2. Maternal numb Does Not Contribute to Notch Signal Downregulation
3.3. The Antineurogenic Phenotype of the numb Mutant Was Dependent on E(spl), a Target Gene of Notch Signaling
3.4. Notch Signaling Is Not Upregulated during Lateral Inhibition in the CNS in numb Mutants
3.5. numb Mutations Impaired Neuronal Projection in the PNS
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genotype | % of Abnormally Short Axons |
---|---|
Wild type | 0% (n = 10) |
numb1 | 38% (n = 10) |
numbEY03840 | 25% (n = 10) |
numbEY03852 | 4% (n = 10) |
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Mujizah, E.Y.; Kuwana, S.; Matsumoto, K.; Gushiken, T.; Aoyama, N.; Ishikawa, H.O.; Sasamura, T.; Umetsu, D.; Inaki, M.; Yamakawa, T.; et al. Numb Suppresses Notch-Dependent Activation of Enhancer of split during Lateral Inhibition in the Drosophila Embryonic Nervous System. Biomolecules 2024, 14, 1062. https://doi.org/10.3390/biom14091062
Mujizah EY, Kuwana S, Matsumoto K, Gushiken T, Aoyama N, Ishikawa HO, Sasamura T, Umetsu D, Inaki M, Yamakawa T, et al. Numb Suppresses Notch-Dependent Activation of Enhancer of split during Lateral Inhibition in the Drosophila Embryonic Nervous System. Biomolecules. 2024; 14(9):1062. https://doi.org/10.3390/biom14091062
Chicago/Turabian StyleMujizah, Elzava Yuslimatin, Satoshi Kuwana, Kenjiroo Matsumoto, Takuma Gushiken, Naoki Aoyama, Hiroyuki O. Ishikawa, Takeshi Sasamura, Daiki Umetsu, Mikiko Inaki, Tomoko Yamakawa, and et al. 2024. "Numb Suppresses Notch-Dependent Activation of Enhancer of split during Lateral Inhibition in the Drosophila Embryonic Nervous System" Biomolecules 14, no. 9: 1062. https://doi.org/10.3390/biom14091062