Cant1 Affects Cartilage Proteoglycan Properties: Aggrecan and Decorin Characterization in a Mouse Model of Desbuquois Dysplasia Type 1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Model and Care
2.2. Chondrocyte Cultures
2.3. Protein Extraction from Chondrocytes
2.4. Proteoglycan Extraction from Cartilage Femoral Head and Skin
2.5. Purification of the Core Protein Standard from Cartilage Proteoglycans
2.6. Western Blot Analysis
2.7. Analysis of Collagen from Femoral Head and Skin
2.8. Immunohistochemical Analysis
3. Results
3.1. Characterization of Decorin in Cant1 Knock-Out Chondrocytes and Tissues
3.2. Characterization of Aggrecan in Cant1 Knock-Out Chondrocytes and Cartilage
3.3. Collagen Analysis in Cartilage and Skin
3.4. Immunohistochemical Localization of Decorin, Aggrecan, and Collagen Type II in the Tibia Epiphysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gramegna Tota, C.; Leone, A.; Khan, A.; Forlino, A.; Rossi, A.; Paganini, C. Cant1 Affects Cartilage Proteoglycan Properties: Aggrecan and Decorin Characterization in a Mouse Model of Desbuquois Dysplasia Type 1. Biomolecules 2024, 14, 1064. https://doi.org/10.3390/biom14091064
Gramegna Tota C, Leone A, Khan A, Forlino A, Rossi A, Paganini C. Cant1 Affects Cartilage Proteoglycan Properties: Aggrecan and Decorin Characterization in a Mouse Model of Desbuquois Dysplasia Type 1. Biomolecules. 2024; 14(9):1064. https://doi.org/10.3390/biom14091064
Chicago/Turabian StyleGramegna Tota, Chiara, Alessandra Leone, Asifa Khan, Antonella Forlino, Antonio Rossi, and Chiara Paganini. 2024. "Cant1 Affects Cartilage Proteoglycan Properties: Aggrecan and Decorin Characterization in a Mouse Model of Desbuquois Dysplasia Type 1" Biomolecules 14, no. 9: 1064. https://doi.org/10.3390/biom14091064