HIV-1 Gag Polyprotein Affinity to the Lipid Membrane Is Independent of Its Surface Charge
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Protein Purification
2.3. Model Lipid Membranes
2.4. Inner Field Compensation Method
2.5. ζ-Potential
3. Results
3.1. Charge Density on Lipid Membranes
3.2. HIV-1 Gag Polyprotein Adsorption onto Lipid Membranes
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lipid Mixture | ζ-Potential, mV | Surface Potential, mV | Charge Density × 10−6 C/cm2 |
---|---|---|---|
1 | −3.7 | −3.9 | −0.09 (≈0) |
2 | −59.8 | −64.9 | −2.3 ± 0.1 |
3 | −28.7 | −30.7 | −0.95 ± 0.02 |
4 | −75.4 | −82.8 | −3.3 ± 0.1 |
5 | −57.7 | −62.5 | −2.1 ± 0.1 |
6 | −76.8 | −84.5 | −3.1 ± 0.1 |
Lipid Mixture | Kads, ×107 M−1 | Kd, nM | Charge Number, z | Reduced χ2 | R2 |
---|---|---|---|---|---|
1 | 0.79 ± 0.04 | 127 ± 6 | 1.0 ± 0.1 | 0.07 | 0.998 |
2 | 0.81 ± 0.04 | 123 ± 6 | 1.11 ± 0.03 | 0.16 | 0.994 |
3 | 1.3 ± 0.2 | 77 ± 12 | 1.21 ± 0.02 | 0.29 | 0.994 |
4 | 0.9 ± 0.1 | 111 ± 12 | 1.12 ± 0.03 | 0.79 | 0.987 |
5 | 2.1 ± 0.2 | 48 ± 5 | 1.0 ± 0.1 | 0.74 | 0.989 |
6 | 2.5 ± 0.1 | 40 ± 2 | 1.02 ± 0.01 | 0.37 | 0.998 |
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Denieva, Z.G.; Sokolov, V.S.; Batishchev, O.V. HIV-1 Gag Polyprotein Affinity to the Lipid Membrane Is Independent of Its Surface Charge. Biomolecules 2024, 14, 1086. https://doi.org/10.3390/biom14091086
Denieva ZG, Sokolov VS, Batishchev OV. HIV-1 Gag Polyprotein Affinity to the Lipid Membrane Is Independent of Its Surface Charge. Biomolecules. 2024; 14(9):1086. https://doi.org/10.3390/biom14091086
Chicago/Turabian StyleDenieva, Zaret G., Valerij S. Sokolov, and Oleg V. Batishchev. 2024. "HIV-1 Gag Polyprotein Affinity to the Lipid Membrane Is Independent of Its Surface Charge" Biomolecules 14, no. 9: 1086. https://doi.org/10.3390/biom14091086