A Putative Effector Pst-18220, from Puccinia striiformis f. sp. tritici, Participates in Rust Pathogenicity and Plant Defense Suppression
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Rust Inoculation
2.2. Cloning and Phylogenetic Analyses of Pst-18220
2.3. RNA Extraction and cDNA Synthesis
2.4. Construction of Plasmids
2.5. HIGS-Mediated Pst-18220 Silencing
2.6. Histological Observations of Pst-18220-Knockdown Wheat Plants
2.7. Transient Expression in N. benthamiana
2.8. Constitutive Overexpression in A. thaliana
2.9. Bacteria Pathogenicity Assay
2.10. H2O2 Accumulation and Observation
2.11. Statistical Analyses
3. Results
3.1. Pst-18220 Is a Pst-Specific Gene
3.2. Pst-18220 Is Highly Induced at the Early Infection Stage of Wheat
3.3. Knocking Down Pst-18220 Expression Compromised the Pathogenicity of Pst
3.4. Pst-18220 Suppressed Leaf Cell Death Mediated by Pseudomonas syringae pv. Tomato (Pto) DC3000 in Nicotiana benthamiana
3.5. Pst-18220 Promotes Pto DC3000 Bacteria Growth in Arabidopsis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tian, M.; Zhang, Z.; Bi, X.; Xue, Y.; Zhou, J.; Yuan, B.; Feng, Z.; Li, L.; Wang, J. A Putative Effector Pst-18220, from Puccinia striiformis f. sp. tritici, Participates in Rust Pathogenicity and Plant Defense Suppression. Biomolecules 2024, 14, 1092. https://doi.org/10.3390/biom14091092
Tian M, Zhang Z, Bi X, Xue Y, Zhou J, Yuan B, Feng Z, Li L, Wang J. A Putative Effector Pst-18220, from Puccinia striiformis f. sp. tritici, Participates in Rust Pathogenicity and Plant Defense Suppression. Biomolecules. 2024; 14(9):1092. https://doi.org/10.3390/biom14091092
Chicago/Turabian StyleTian, Mengfan, Zhen Zhang, Xiaorui Bi, Yan Xue, Jiahui Zhou, Bo Yuan, Zhaozhong Feng, Lianwei Li, and Junjuan Wang. 2024. "A Putative Effector Pst-18220, from Puccinia striiformis f. sp. tritici, Participates in Rust Pathogenicity and Plant Defense Suppression" Biomolecules 14, no. 9: 1092. https://doi.org/10.3390/biom14091092