Insights into the Metabolite Profiles of Two Camellia (Theaceae) Species in Yunnan Province through Metabolomic and Transcriptomic Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Observation and Determination of Morphological Characteristics
2.3. Determination of Tea Physicochemical Indices
2.3.1. Determination of Aqueous Extraction
- m0—the weight of the tea powder, g;
- m1—the weight of the dried tea residue, g;
- ω—the dry matter content of the tea sample (mass fraction), %.
2.3.2. Determination of Total Tea Polyphenols
- A—absorbance of test solution;
- V—volume of sample extract, mL;
- d—dilution factor;
- SLOPEstd—slope of the standard curve for gallic acid;
- ω—the dry matter content of the tea sample (mass fraction), %
- m—the weight of the tea sample, g.
2.3.3. Determination of Total Free Amino Acids
- C—the theanine weight, which could be obtained according to the OD570 from a standard curve made by theanine as a standard component, using the same method as mentioned above, mg;
- V1—the total volume of the tea infusion, mL;
- V2—the volume of the infusion taken to reaction, mL;
- m—the dry weight of the tea sample, g;
- w—the dry ratio of the tea sample, %.
2.3.4. Quantification of Catechins and Caffeine via HPLC
2.3.5. Quantification of Amino Acids Using HPLC
2.4. Widely Targeted Metabolite Identification and Quantification
2.4.1. Dry Sample Extraction
2.4.2. UPLC Conditions
2.4.3. ESI-Q TRAP–MS/MS
2.4.4. Data Analysis
2.5. RNA Sequencing (RNA-seq) Analysis and Differentially Expressed Genes (DEGs)
3. Results
3.1. Morphological Characteristics
3.2. Physicochemical Indices in DL and QJZ
3.3. Metabolome Profiling
3.4. Differentially Accumulated Metabolites (DAMs) Analysis of Two Tea Species
3.5. Comparative Transcriptome Analysis of Two Tea Species
3.6. Conjoint Analysis of Transcriptomics and Metabolomics Data
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Niu, M.; Li, R.; Li, X.; Yang, H.; Ding, J.; Zhou, X.; He, Y.; Xu, Y.; Qu, Q.; Liu, Z.; et al. Insights into the Metabolite Profiles of Two Camellia (Theaceae) Species in Yunnan Province through Metabolomic and Transcriptomic Analysis. Biomolecules 2024, 14, 1106. https://doi.org/10.3390/biom14091106
Niu M, Li R, Li X, Yang H, Ding J, Zhou X, He Y, Xu Y, Qu Q, Liu Z, et al. Insights into the Metabolite Profiles of Two Camellia (Theaceae) Species in Yunnan Province through Metabolomic and Transcriptomic Analysis. Biomolecules. 2024; 14(9):1106. https://doi.org/10.3390/biom14091106
Chicago/Turabian StyleNiu, Miao, Ranyang Li, Xiongyu Li, Hongyan Yang, Jianliang Ding, Xianxiu Zhou, Yuqi He, Yawen Xu, Qian Qu, Zhiwei Liu, and et al. 2024. "Insights into the Metabolite Profiles of Two Camellia (Theaceae) Species in Yunnan Province through Metabolomic and Transcriptomic Analysis" Biomolecules 14, no. 9: 1106. https://doi.org/10.3390/biom14091106