FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis and Characterization of Fluorescent Magnetic Submicronic Polymer-Nanoparticles
2.1.1. Preparation of the cationic magnetic emulsion
2.1.2. Polyethyleneimine adsorption study
2.1.3. Hetero-coagulation step
2.1.4. Film-formation step
2.1.5. Characterization of FMSP-nanoparticles
2.2. Treatment of Fluorescent Magnetic Submicronic Polymer-Nanoparticles
2.3. Morphometric Analysis
2.4. 4’,6-Diamidino-2-phenylindole Staining
2.5. Statistical Analysis
3. Results
3.1. Characterization of Fluorescent Magnetic Submicronic Polymer-Nanoparticles
3.2. Morphology of the Fluorescent Magnetic Submicronic Polymer-Nanoparticles Treated MCF-7 Cells
3.3. Cell Death Analysis by 4’,6-diamidino-2-phenylindole Staining
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Availability of Data and Material
References
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Reference | ME1 | ME2 |
---|---|---|
Dh (nm) a | 246 | 249 |
Dn (nm) b | 219 | 221 |
Dw (nm) b | 233 | 232 |
Polydispersity index b | 1.066 | 1.050 |
Solid content (%) | 6.1 | 9.7 |
Density (g cm−3) | 2.66 | 2.42 |
Magnetic content (wt %) | 70 | 70 |
Reference | Rhodopas PR 3500 | Yellow-Green Fluospheres |
---|---|---|
Dh (nm) a | 47 | 24 |
Polymer nature | Acrylic copolymer (Tg | Polystyren (Tg |
Density (g cm−3) | 1.03 d | 1.04 c |
Fluorescence | / | λ exc = 505 nm c em = 515 nm c |
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Khan, F.A.; Akhtar, S.; Almofty, S.A.; Almohazey, D.; Alomari, M. FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis. Biomolecules 2018, 8, 32. https://doi.org/10.3390/biom8020032
Khan FA, Akhtar S, Almofty SA, Almohazey D, Alomari M. FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis. Biomolecules. 2018; 8(2):32. https://doi.org/10.3390/biom8020032
Chicago/Turabian StyleKhan, Firdos Alam, Sultan Akhtar, Sarah Ameen Almofty, Dana Almohazey, and Munthar Alomari. 2018. "FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis" Biomolecules 8, no. 2: 32. https://doi.org/10.3390/biom8020032