IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication
Abstract
:1. Introduction
2. Materials and Methods
2.1. RNA Pull-Down Assay
2.2. Plasmids
2.3. In Vitro Transcription
2.4. Cells and Transfection
2.5. In Vitro RNA Pull-Down
2.6. Establishing a Stable DEE Cell Line Continuously Expressing IGF2BP1
2.7. RNA Interference (RNAi)
2.8. Luciferase Activities Measurement
2.9. Western Blot and Antibodies
2.10. Immunofluorescence Assays
2.11. Quantitative Real Time-Polymerase Chain Reaction
2.12. Statistical Analyses
3. Results
3.1. IGF2BP1 Specifically Interacted with 3′ UTR of DHAV-1
3.2. IGF2BP1 Strongly Enhances DHAV-1 IRES-Mediated Translation through Binding to 3′ UTR
3.3. Viral Protein Accumulation Significantly Increases IGF2BP1 Expression Level
3.4. IGF2BP1 Does Not Affect DHAV-1 Replication
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Chen, J.; Zhang, R.; Lan, J.; Lin, S.; Li, P.; Gao, J.; Wang, Y.; Xie, Z.-J.; Li, F.-C.; Jiang, S.-J. IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication. Biomolecules 2019, 9, 594. https://doi.org/10.3390/biom9100594
Chen J, Zhang R, Lan J, Lin S, Li P, Gao J, Wang Y, Xie Z-J, Li F-C, Jiang S-J. IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication. Biomolecules. 2019; 9(10):594. https://doi.org/10.3390/biom9100594
Chicago/Turabian StyleChen, Junhao, Ruihua Zhang, Jingjing Lan, Shaoli Lin, Pengfei Li, Jiming Gao, Yu Wang, Zhi-Jing Xie, Fu-Chang Li, and Shi-Jin Jiang. 2019. "IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication" Biomolecules 9, no. 10: 594. https://doi.org/10.3390/biom9100594