Detection and Identification of Allergens from Canadian Mustard Varieties of Sinapis alba and Brassica juncea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Mustard Seed Flours
2.2. Optimization of Mustard Seed Protein Extraction
2.3. Protein Electrophoresis
2.4. Immunoblotting
2.5. Indirect ELISA
2.6. Identification of Protein Bands as Allergens by LC-MS/MS
2.7. Protein Sequence Comparisons with Known and Putative Allergens
3. Results and Discussion
3.1. Protein Content and Extractability from Different Mustard Varieties
3.2. Protein Electrophoretic Profiles of Differents Mustard Varieties as a Function of Extraction Buffer
3.3. Indirect ELISA for Serum IgE Response to Mustard Varieties
3.4. IgE-Binding Profiles of Select Canadian Mustard Varieties
3.5. Identification of Mustard IgE-Binding Proteins by Mass Spectroscopy
3.6. Bioinformatic Assessment of Potential Cross-Reactivity of Identified Mustard IgE-Binding Proteins with Known Allergens
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Band Intensity (1 × 106) | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sinapis alba | Brassica juncea | |||||||||||||||||||||||
MW a | AC Pennant | Andante | MW a | Duchess | Centennial Brown | Vulcan | Cutlass | Dahinda | ||||||||||||||||
Band Name | kDa | 1 | 2 | 3 | 1 | 2 | 3 | Band Name | kDa | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 |
Sin a 1 LC b | 9.5 | 24.1 | 18.9 | 19.1 | 23.4 | 18.3 | 18.3 | Bra j 1 LC b | 9.5 | 23.7 | 15.0 | 19.6 | 23.7 | 13.0 | 15.7 | 22.4 | 18.1 | 19.9 | 27.2 | 16.7 | 21.2 | 26.4 | 23.1 | 22.5 |
Sin a 1 HC c | 12 | 25.0 | 19.2 | 19.4 | 11.8 | 19.4 | 19.1 | Bra j 1 HC c | 12 | 19.2 | 11.1 | 14.1 | 17.7 | 10.0 | 18.0 | 13.9 | 12.9 | 14.7 | 19.6 | 10.8 | 15.0 | 16.1 | 13.9 | 14.6 |
Unknown | 15.1 | 0.6 | 2.8 | 0.8 | 2.1 | Unknown | 14.6 | 2.4 | 1.2 | 3.3 | 1.1 | 2.8 | 1.1 | 3.7 | 0.8 | |||||||||
Cruciferin | Cruciferin | 16.8 | 7.1 | 6.8 | 5.5 | 5.4 | 7.3 | 8.2 | 6.8 | 6.0 | 3.5 | 10.5 | 9.9 | |||||||||||
β-polypeptides | 18 | 13.3 | 14.8 | 15.0 | 11.8 | 14.2 | 10.6 | β-polypeptides | 18 | 11.3 | 10.3 | 8.7 | 12.7 | 11.8 | 10.9 | 13.9 | 12.3 | 9.7 | 14.8 | 12.5 | 11.8 | 13.7 | 14.3 | 13.3 |
11 S globulin | 19.1 | 5.9 | 11.6 | 9.6 | 6.4 | 12.0 | 5.1 | 11 S globulin | 19.1 | 5.7 | 6.7 | 5.2 | 5.7 | 6.4 | 5.3 | 6.6 | 7.9 | 5.9 | 5.7 | 6.5 | 4.7 | 6.7 | 8.2 | 7.0 |
Sin a 2 | 21.6 | 8.8 | 12.6 | 10.5 | 9.0 | 12.5 | 7.8 | Sin a 2 | 20.9 | 6.3 | 6.1 | 5.2 | 7.5 | 7.1 | 6.5 | 7.2 | 6.5 | 5.2 | 7.2 | 6.4 | 5.4 | 7.9 | 7.7 | 6.7 |
Cruciferin | Cruciferin | 23.7 | 1.4 | 1.3 | 2.3 | 2.2 | 1.7 | 2.2 | 1.9 | 1.6 | 2.2 | 1.9 | 1.6 | 2.6 | 2.7 | 2.7 | ||||||||
α-polypeptides | 26.8 | 6.2 | 7.0 | 6.5 | 4.4 | 4.2 | 4.7 | α-polypeptides | 25.3 | 10.5 | 10.4 | 10.4 | 13.0 | 12.6 | 12.1 | 13.7 | 12.5 | 10.7 | 14.1 | 12.9 | 11.9 | 11.1 | 10.4 | 10.0 |
11S globulin | 28.4 | 10.6 | 13.2 | 11.7 | 11.7 | 14.1 | 12.3 | 11S globulin | 28 | 2.6 | 2.3 | 1.6 | 2.8 | 3.1 | 2.5 | 2.7 | 3.0 | 2.1 | 2.8 | 2.8 | 2.1 | 3.7 | 3.5 | 3.1 |
Sin a 2 | 30.9 | 1.3 | 0.8 | Sin a 2 | 30.5 | 9.6 | 9.1 | 7.3 | 12.0 | 10.6 | 9.8 | 10.2 | 8.7 | 7.2 | 10.2 | 8.7 | 7.2 | 6.2 | 5.6 | 5.0 | ||||
32.9 | 10.5 | 11.0 | 9.4 | 10.0 | 10.4 | 9.3 | ||||||||||||||||||
11S fragment | 55 | 1.7 | 1.5 | 1.9 | 1.6 | 11S fragment | 52 | 1.0 | 0.5 | 1.1 | 1.4 | 1.3 | 0.8 | 1.0 | 0.9 | 1.1 | 1.2 | 1.0 | 1.8 | 2.4 | 1.3 | |||
Procruciferin | 63.5 | 1.0 | 1.0 | 1.1 | 0.9 | 1.6 | 3.0 | Procruciferin | 63.6 | 2.9 | 1.1 | 1.6 | 1.5 | 1.3 | 1.5 | 1.7 | 1.7 | 1.3 | 1.2 | 2.0 | 3.0 | 1.0 | ||
66.8 | 2.0 | 1.5 | 2.0 | 2.7 | 65.9 | 3.8 | 2.6 | 2.3 | 1.5 | 1.1 | 1.1 | 1.3 | 1.2 | |||||||||||
69.4 | 3.4 | 5.9 | 5.2 | 2.0 | 3.2 | 2.0 | 69.9 | 2.0 | 1.8 | 1.2 | 1.1 | 1.9 | 2.4 | 2.0 | 1.2 | |||||||||
73.9 | 2.7 | 2.5 | 1.7 | 2.3 | 3.3 | 72.5 | 2.7 | 1.0 | 1.3 | 2.8 | 2.3 | 2.4 | 1.4 | 3.4 | 1.2 |
Band Number a | MW on SDS-PAGE (KDa) | Protein Name b | Protein Accession Numbers | Protein MW (KDa) | Exclusive Unique Peptide Count | Percentage Sequence Coverage c |
---|---|---|---|---|---|---|
Sinapis alba (AC Pennant) | ||||||
S1 | 16 | ALL1_SINAL Allergen Sin a 1 [Sinapis alba (White mustard)] | P15322 | 16.4 | 5 | 23.40% |
S2 | 28 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 16 | 39.40% |
S3 | 30 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 16 | 39.40% |
CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 13 | 30.10% | ||
S4 | 44 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 19 | 44.30% |
CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 14 | 29.70% | ||
S5 | 75 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 18 | 44.30% |
CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 7 | 23.40% | ||
S6 | 107 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 21 | 46.50% |
S7 | 223 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 20 | 39.40% |
BRANA Beta-glucosidase [Brassica napus (Rape)] | Q42618 | 56.5 | 20 | 34.40% | ||
RS1 | 9.5 | ALL1_SINAL Allergen Sin a 1 [Sinapis alba (White mustard)] | P15322 | 16.4 | 7 | 26.90% |
RS2 | 12 | ALL1_SINAL Allergen Sin a 1 [Sinapis alba (White mustard)] | P15322 | 16.4 | 9 | 57.90% |
RS3 | 20 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 19 | 36.10% |
BRANA Cruciferin (Fragment) [Brassica napus (Rape)] | Q7XB53 | 51.3 | 8 | 23.20% | ||
RS4 | 28 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 14 | 25.90% |
CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 7 | 27.50% | ||
RS5 | 34 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 14 | 25.90% |
SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLV9 | 57.9 | 5 | 34.60% | ||
RS6 | 44 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 18 | 46.70% |
RS7 | 55 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 15 | 37.60% |
CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 10 | 25.20% | ||
RS8 | 62 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 20 | 45.10% |
RS9 | 75 | Q2TLW0_SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 16 | 40.20% |
BRANA Beta-glucosidase [Brassica napus (Rape)] | Q42618 | 58.5 | 12 | 20.80% | ||
RS10 | 82 | Q2TLW0_SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 14 | 37.10% |
RS11 | 118 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 12 | 27.50% |
RS12 | 140 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 9 | 24.50% |
Brassicae juncea (AC Vulcan) | ||||||
B1 | 15.5 | Allergen Bra j 1-E [Brassica juncea (Indian mustard)] | P80207 | 14.6 | 5 | 51.90% |
OLES2_BRANA Oleosin S2-2 [Brassica napus (Rape)] | C3S7F1 | 19.9 | 15 | 46.80% | ||
B2 | 17 | BRANA Oleosin S3-1 [Brassica napus (Rape)] | C3S7F8 | 19.6 | 10 | 41.70% |
OLES2_BRANA Oleosin S2-2 [Brassica napus (Rape)] | C3S7F1 | 19.9 | 16 | 46.80% | ||
B3 | 26 | BRANA Caleosin CLO1-2 [Brassica napus (Rape)] | C3S7H5 | 28.1 | 7 | 35.90% |
CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 21 | 49.50% | ||
B4 | 30 | CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 12 | 47.20% |
OLES2_BRANA Oleosin S2-2 [Brassica napus (Rape)] | C3S7F1 | 19.9 | 11 | 46.30% | ||
B5 | 29 | BRANA Cruciferin (Fragment) [Brassica napus (Rape)] | Q7XB53 | 51.3 | 4 | 8.80% |
CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 8 | 24.30% | ||
B6 | 34 | SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 7 | 20.80% |
CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 3 | 21.80% | ||
B7 | 42.5 | CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 12 | 45.20% |
CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 20 | 52.30% | ||
B8 | 68 | CRU3_BRANA Cruciferin CRU1 [Brassica napus] | P33525 | 56.5 | 14 | 52.80% |
CRU4_BRANA Cruciferin CRU4 [Brassica napus] | P33522 | 51.4 | 13 | 40.90% | ||
B9 | 200 | Malate synthase. glyoxysomal [Brassica napus (Rape)] | P13244 | 63.7 | 14 | 23.00% |
BRANA Beta-glucosidase [Brassica napus (Rape)] | Q42618 | 58.5 | 23 | 44.20% | ||
RB1 | 9.5 | Allergen Bra j 1-E [Brassica juncea (Indian mustard)] | P80207 | 14.6 | 2 | 38.00% |
RB2 | 12 | Allergen Bra j 1-E [Brassica juncea (Indian mustard)] | P80207 | 14.6 | 5 | 68.20% |
ALL1_SINAL Allergen Sin a 1 [Sinapis alba (White mustard)] | P15322 | 16.5 | 4 | 35.20% | ||
RB3 | 22 | CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 11 | 39.50% |
CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 11 | 35.30% | ||
RB4 | 27 | BRANA Caleosin CLO1-2 [Brassica napus (Rape)] | C3S7H5 | 28.1 | 8 | 37.60% |
OLES2_BRANA Oleosin S2-2 [Brassica napus (Rape)] | C3S7F1 | 20.0 | 6 | 40.40% | ||
BRAJU Glutathione S-transferase 3 [Brassica juncea] | Q7XZT2 | 24.1 | 7 | 46.00% | ||
RB5 | 30 | CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 17 | 42.60% |
RB6 | 30 | CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 12 | 45.20% |
CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 15 | 38.70% | ||
RB7 | 34 | CRU3_BRANA Cruciferin CRU1 [Brassica napus (Rape)] | P33525 | 56.5 | 8 | 28.90% |
BRAJU Cysteine synthase [Brassica juncea] | O23733 | 33.9 | 8 | 33.20% | ||
RB8 | 47 | CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 25 | 54.00% |
BRANA Cruciferin (Fragment) [Brassica napus (Rape)] | Q7XB53 | 51.3 | 14 | 39.70% | ||
RB9 | 52 | CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 16 | 45.40% |
BRACM Enolase [Brassica campestris (Field mustard)] | Q6W7E8 | 47.4 | 21 | 61.00% | ||
RB10 | 62 | BRANA Beta-glucosidase [Brassica napus (Rape)] | Q42618 | 58.5 | 13 | 30.00% |
RB11 | 76 | CRU4_BRANA Cruciferin CRU4 [Brassica napus (Rape)] | P33522 | 51.4 | 8 | 30.10% |
SINAL 11S globulin [Sinapis alba (White mustard)] | Q2TLW0 | 56.5 | 5 | 15.30% |
Protein Identification a | Species | Best %ID b | # Hits >35% | Full Alignment | NCBI Links f | ||
---|---|---|---|---|---|---|---|
E-value c | %ID d | Length e | |||||
P15322: Allergen Sin a 1 [Sinapis alba (White mustard)] | |||||||
gid|192|Allergen Allergen Sin a 1 precursor | Sinapis alba | 100.00% | 66 of 66 | 2.4e-34 | 100.0% | 145 | gi|51338758 |
gid|1172|Putative 2S storage protein | Brassica rapa | 93.80% | 66 of 66 | 2.5e-29 | 88.30% | 145 | gi|17697 |
gid|1142|Putative Napin-3 | Brassica napus | 91.20% | 66 of 66 | 2.6e-17 | 82.60% | 144 | gi|75107016 |
gid|1170|Putative Allergen Bra j 1-E | Brassica juncea | 87.50% | 66 of 66 | 1.8e-17 | 79.90% | 144 | gi|32363444 |
P80207: Allergen Bra j 1-E [Brassica juncea (Indian mustard)] | |||||||
gid|1170|Putative Allergen Bra j 1-E (Bra j 1) | Brassica juncea | 100.00% | 50 of 50 | 7.1e-23 | 100.0% | 129 | gi|32363444 |
gid|1142|Putative Napin-3 (Napin BnIII) | Brassica napus | 92.50% | 50 of 50 | 8.9e-20 | 89.10% | 129 | gi|75107016 |
gid|192|Allergen allergen sin a 1.0104 | Sinapis alba | 88.78% | 50 of 50 | 2.5e-13 | 80.60% | 144 | gi|1009434 |
gid|1172|Putative 2S storage protein | Brassica rapa | 85.00% | 50 of 50 | 1e-11 | 77.10% | 144 | gi|17697 |
gid|386|Putative recombinant Ib pronapin | Brassica napus | 55.00% | 50 of 50 | 3.8e-6 | 50.00% | 114 | gi|26985163 |
Q2TLWO: 11S globulin [Sinapis alba (White mustard)] | |||||||
gid|837|Allergen 11S globulin precursor | Sinapis alba | 100.00% | 431 of 431 | 0 | 100.0% | 510 | gi|62240390 |
gid|2066|Putative 11S legumin protein | Carya illinoinensis | 62.51% | 351 of 431 | 2.6e-61 | 43.20% | 521 | gi|158998782 |
gid|2597|Putative legumin | Juglans nigra | 62.51% | 362 of 431 | 2.7e-58 | 42.00% | 528 | gi|1126299828 |
gid|76|Allergen allergen Ana 0 2 | Anacardium occidentale | 60.04% | 380 of 431 | 2.3e-70 | 44.80% | 498 | gi|25991543 |
gid|160|Allergen HMW glutenin | Triticum aestivum | 60.00% | 84 of 431 | 3.5e-18 | 37.60% | 213 | gi|288860106 |
gid|392|Allergen 11S globulin | Corylus avellana | 58.00% | 387 of 431 | 3.6e-61 | 45.70% | 534 | gi|18479082 |
gid|1572|Putative prunin 2 precursor | Prunus dulcis | 57.80% | 418 of 431 | 3e-61 | 45.60% | 522 | gi|307159114 |
gid|2650|Allergen 11S globulin- | Actinidia chinensis | 57.50% | 352 of 431 | 2.3e-68 | 41.40% | 503 | gi|82469930 |
gid|2274|Putative 11S globulin | Sesamum indicum | 55.00% | 342 of 431 | 3.7e-60 | 42.10% | 470 | gi|13183173 |
gid|1093|Putative 11S globulin | Pistacia vera | 55.00% | 356 of 431 | 6.5e-53 | 41.80% | 505 | gi|156001070 |
gid|531|Putative allergenic protein | Fagopyrum tataricum | 55.00% | 256 of 431 | 1.5e-43 | 38.10% | 486 | gi|113200131 |
gid|733|Putative glycinin subunit G3 | Glycine max | 53.10% | 272 of 431 | 2e-38 | 36.20% | 514 | gi|18639 |
gid|347|Putative 11S globulin | Bertholletia excelsa | 52.50% | 318 of 431 | 5.8e-59 | 39.20% | 510 | gi|30313867 |
Q7XB53: Cruciferin (Fragment) [Brassica napus (Rape)] | |||||||
gid|837|Allergen 11S globulin precursor | Sinapis alba | 76.50% | 387 of 387 | 3.5e-29 | 61.00% | 472 | gi|62240390 |
gid|392|Allergen Cor a 9 allergen | Corylus avellana | 63.70% | 353 of 387 | 9.4e-22 | 44.00% | 509 | gi|557792009 |
gid|1572|Putative Pru du 6 allergen | Prunus dulcis | 62.51% | 160 of 387 | 3.2e-10 | 48.20% | 193 | gi|523916668 |
gid|76|Allergen allergen Ana 0 2 | Anacardium occidentale | 61.30% | 333 of 387 | 1.2e-22 | 44.70% | 474 | gi|25991543 |
gid|2597|Putative legumin | Juglans nigra | 58.79% | 298 of 387 | 3.8e-21 | 42.70% | 503 | gi|1126299828 |
gid|2066|Putative 11S legumin protein | Carya illinoinensis | 58.79% | 326 of 387 | 3.1e-21 | 43.50% | 506 | gi|158998782 |
gid|817|Putative seed storage protein | Juglans regia | 57.52% | 325 of 387 | 4.4e-21 | 42.90% | 513 | gi|56788031 |
gid|1093|Putative Pis v 2.0201 allergen 11S | Pistacia vera | 56.30% | 347 of 387 | 1.7e-33 | 41.60% | 459 | gi|110349085 |
gid|347|Putative 11S globulin | Bertholletia excelsa | 53.80% | 349 of 387 | 1.2e-24 | 43.30% | 466 | gi|30313867 |
gid|733|Putative glycinin subunit G3 | Glycine max | 53.80% | 274 of 387 | 4.8e-22 | 38.00% | 479 | gi|18639 |
gid|2274|Putative 11S globulin | Sesamum indicum | 52.46% | 318 of 387 | 1.6e-24 | 40.80% | 476 | gi|13183173 |
gid|291|Allergen trypsin inhibitor | Arachis hypogaea | 49.40% | 59 of 387 | 1.9e-5 | 35.80% | 204 | gi|22135348 |
P33525: Cruciferin CRU4 [Brassica napus (Rape) | |||||||
gid|837|Allergen 11S globulin precursor | Sinapis alba | 98.80% | 430 of 430 | 2.4e-210 | 91.60% | 510 | gi|62240390 |
gid|2066|Putative 11S legumin protein | Carya illinoinensis | 65.00% | 358 of 430 | 1.9e-61 | 44.70% | 535 | gi|158998782 |
gid|2597|Putative legumin | Juglans nigra | 63.79% | 373 of 430 | 4.1e-61 | 43.30% | 533 | gi|1126299828 |
gid|817|Putative seed storage protein | Juglans regia | 63.79% | 368 of 430 | 1e-63 | 44.50% | 533 | gi|56788031 |
gid|1572|Putative Pru du 6 allergen | Prunus dulcis | 60.04% | 168 of 430 | 4.6e-20 | 49.70% | 195 | gi|523916668 |
gid|76|Allergen allergen Ana 0 2 | Anacardium occidentale | 60.04% | 379 of 430 | 7.9e-73 | 46.20% | 487 | gi|25991543 |
gid|392|Allergen Cor a 9 allergen | Corylus avellana | 58.79% | 391 of 430 | 4.6e-62 | 45.50% | 528 | gi|557792009 |
gid|160|Allergen glutenin | Triticum aestivum | 56.26% | 70 of 430 | 2.5e-11 | 33.30% | 207 | gi|736319 |
gid|1093|Putative Pis v 2.0101 11S globulin | Pistacia vera | 55.00% | 345 of 430 | 6.7e-51 | 41.10% | 518 | gi|110349083 |
gid|2274|Putative 11S globulin | Sesamum indicum | 53.80% | 343 of 430 | 4.8e-63 | 42.10% | 478 | gi|13183173 |
gid|531|Putative allergenic protein | Fagopyrum tataricum | 53.80% | 255 of 430 | 4.4e-44 | 35.60% | 523 | gi|113200131 |
gid|347|Putative 11S globulin | Bertholletia excelsa | 52.50% | 309 of 430 | 2.3e-59 | 39.70% | 506 | gi|30313867 |
gid|733|Putative glycinin subunit G3 | Glycine max | 51.20% | 267 of 430 | 3e-39 | 36.00% | 516 | gi|18639 |
gid|291|Allergen allergen Arah3/Arah4 | Arachis hypogaea | 47.50% | 219 of 430 | 2.1e-25 | 33.20% | 548 | gi|21314465 |
gid|574|Putative glycinin precursor | Glycine max | 47.50% | 116of430 | 4.9e-28 | 39.80% | 191 | gi|169971 |
Q2TLV9: 11S globulin [Sinapis alba (White mustard)] | |||||||
gid|837|Allergen 11S globulin precursor | Sinapis alba | 100.00% | 444 of 444 | 0 | 100.00% | 523 | gi|62240392 |
gid|160|Allergen HMW glutenin | Triticum aestivum | 68.80% | 99 of 444 | 1.3e-12 | 38.60% | 228 | gi|288860106 |
gid|2066|Putative 11S legumin protein | Carya illinoinensis | 63.79% | 360 of 444 | 6.3e-41 | 41.90% | 532 | gi|158998782 |
gid|817|Putative seed storage protein | Juglans regia | 62.51% | 367 of 444 | 1.1e-41 | 41.70% | 545 | gi|56788031 |
gid|2597|Putative legumin | Juglans nigra | 62.51% | 364 of 444 | 2.5e-41 | 40.80% | 539 | gi|1126299828 |
gid|76|Allergen allergen Ana 0 2 | Anacardium occidentale | 61.30% | 380 of 444 | 9.9e-70 | 42.70% | 510 | gi|25991543 |
gid|1572|Putative Chain A | Prunus dulcis | 59.30% | 417 of 444 | 6.5e-53 | 45.20% | 524 | gi|258588247 |
gid|392|Allergen Cor a 9 allergen | Corylus avellana | 59.30% | 390 of 444 | 3.4e-41 | 43.70% | 545 | gi|557792009 |
gid|1572|Putative prunin 1 precursor | Prunus dulcis | 59.30% | 436 of 444 | 2.6e-53 | 44.90% | 543 | gi|307159112 |
gid|160|Allergen HMW glutenin | Triticum aestivum | 57.52% | 76 of 444 | 4.2e-9 | 31.30% | 307 | gi|21751 |
gid|1572|Putative Pru du 6 allergen | Prunus dulcis | 57.50% | 168 of 444 | 4.8e-20 | 46.20% | 208 | gi|523916668 |
gid|2650|Allergen 11S globulin | Actinidia chinensis | 56.30% | 344 of 444 | 4.1e-67 | 39.70% | 514 | gi|82469930 |
gid|1093|Putative Pis v 2.0201 allergen 11S | Pistacia vera | 56.30% | 332 of 444 | 9.2e-53 | 39.50% | 516 | gi|110349085 |
gid|2274|Putative 11S globulin | Sesamum indicum | 53.80% | 345 of 444 | 2e-60 | 41.10% | 482 | gi|13183173 |
gid|574|Putative glycinin A3B4 subunit | Glycine max | 53.80% | 241 of 444 | 6.4e-27 | 32.40% | 558 | gi|10566449 |
gid|291|Allergen allergen Arah3/Arah4 | Arachis hypogaea | 47.50% | 208 of 444 | 1.2e-20 | 32.40% | 561 | gi|21314465 |
C3S7F1: Oleosin S2-2 [Brassica napus (Rape)] | |||||||
gid|2298|Allergen oleosin | Corylus avellana | 70.01% | 109 of 109 | 2.2e-42 | 52.50% | 158 | gi|49617323 |
gid|2283|Putative oleosin 1 | Arachis hypogaea | 64.98% | 104 of 109 | 6.5e-39 | 46.20% | 171 | gi|113200509 |
gid|1893|Putative oleosin | Sesamum indicum | 62.50% | 103 of 109 | 2.2e-31 | 45.90% | 157 | gi|10834827 |
gid|389|Putative oleosin | Corylus avellana | 50.00% | 68 of 109 | 1.1e-21 | 41.00% | 117 | gi|29170509 |
gid|2285|Allergen oleosin 3 | Arachis hypogaea | 45.10% | 77 of 109 | 9.1e-21 | 38.20% | 144 | gi|52001241 |
C3S7F8: Oleosin S3-1 [Brassica napus (Rape] | |||||||
gid|1238|Putative 15 kDa oleosin | Sesamum indicum | 76.20% | 101 of 101 | 6e-31 | 63.20% | 125 | gi|5381321 |
gid|389|Putative oleosin | Corylus avellana | 73.80% | 98 of 101 | 1.1e-32 | 66.90% | 121 | gi|29170509 |
gid|2284|Putative oleosin 1 | Arachis hypogaea | 70.00% | 94 of 101 | 1.2e-28 | 60.80% | 125 | gi|71040655 |
Q6W7E8: Enolase [Brassica campestris (Field mustard)] | |||||||
gid|586|Putative Enolase | Hevea brasiliensis | 98.80% | 365 of 365 | 1.1e-170 | 89.60% | 442 | gi|14423687 |
gid|1955|Allergen Alpha-enolase | Thunnus albacares | 88.70% | 365 of 365 | 2.9e-129 | 69.50% | 442 | gi|576011129 |
gid|1959|Allergen enolase | Salmo salar | 88.70% | 365 of 365 | 5.1e-130 | 69.90% | 442 | gi|385145180 |
gid|2710|Allergen beta-enolase | Gallus gallus | 83.80% | 365 of 365 | 3.6e-119 | 65.10% | 441 | gi|46048765 |
gid|396|Allergen Enolase | Candida albicans | 82.50% | 365 of 365 | 1.5e-111 | 63.10% | 444 | gi|232054 |
gid|103|Putative enolase | Rhodotorula mucilaginosa | 80.00% | 365 of 365 | 9.5e-107 | 61.00% | 441 | gi|30314940 |
Q7XZT2-BRAJU Glutathione S-transferase 3 [Brassica juncea] | |||||||
gid|856|Allergen glutathione S transferase c | Periplaneta americana | 40.00% | 14 of 134 | 1.2e-6 | 29.40% | 163 | gi|359326557 |
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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L’Hocine, L.; Pitre, M.; Achouri, A. Detection and Identification of Allergens from Canadian Mustard Varieties of Sinapis alba and Brassica juncea. Biomolecules 2019, 9, 489. https://doi.org/10.3390/biom9090489
L’Hocine L, Pitre M, Achouri A. Detection and Identification of Allergens from Canadian Mustard Varieties of Sinapis alba and Brassica juncea. Biomolecules. 2019; 9(9):489. https://doi.org/10.3390/biom9090489
Chicago/Turabian StyleL’Hocine, Lamia, Mélanie Pitre, and Allaoua Achouri. 2019. "Detection and Identification of Allergens from Canadian Mustard Varieties of Sinapis alba and Brassica juncea" Biomolecules 9, no. 9: 489. https://doi.org/10.3390/biom9090489