DnaJ Proteins Regulate WUS Expression in Shoot Apical Meristem of Arabidopsis
Abstract
:1. Introduction
2. Results and Discussion
2.1. WUS Interacts with DnaJ Proteins In Vivo and In Vitro
2.2. DnaJ Proteins Function as a Co-Factor of WUS in Activating WUS Expression
2.3. Disruption of DnaJ Proteins by Chimeric Repressor Silencing Technology Led to Premature Termination of SAM
2.4. SDJ1 and SDJ3 Genes are Expressed in the SAM, RAM and Vascular Tissues of Leaf and Hypocotyl
2.5. DnaJ Proteins Contribute to the Expression of WUS mRNA
3. Materials and Methods
3.1. Plant Materials and Transformation
3.2. Plasmids Construction for Transgenic Plants
3.3. Protein Purification
3.4. Yeast Two-Hybrid Assay
3.5. In Vitro Pull-Down Assay
3.6. Bimolecular Fluorescence Complementation (BiFC) Assays
3.7. Co-Immunoprecipitation
3.8. Chromatin Immunoprecipitation (ChIP)
3.9. Electrophoretic Mobility Shift Assay (EMSA)
3.10. Transactivation Assay in Tobacco
3.11. Subcellular Localization of DnaJ Proteins
3.12. RNA Isolation and Real-Time RT-PCR
3.13. Histochemical Localization of GUS Activity
3.14. Accession Numbers
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Jia, T.; Li, F.; Liu, S.; Dou, J.; Huang, T. DnaJ Proteins Regulate WUS Expression in Shoot Apical Meristem of Arabidopsis. Plants 2021, 10, 136. https://doi.org/10.3390/plants10010136
Jia T, Li F, Liu S, Dou J, Huang T. DnaJ Proteins Regulate WUS Expression in Shoot Apical Meristem of Arabidopsis. Plants. 2021; 10(1):136. https://doi.org/10.3390/plants10010136
Chicago/Turabian StyleJia, Tianqi, Fan Li, Shuang Liu, Jin Dou, and Tao Huang. 2021. "DnaJ Proteins Regulate WUS Expression in Shoot Apical Meristem of Arabidopsis" Plants 10, no. 1: 136. https://doi.org/10.3390/plants10010136