Transmission of Engineered Plastids in Sugarcane, a C4 Monocotyledonous Plant, Reveals that Sorting of Preprogrammed Progenitor Cells Produce Heteroplasmy
Abstract
:1. Introduction
2. Results
2.1. Development of a Proficient Regeneration Protocol
2.2. Development of Species-Specific Chloroplast Transformation Vectors
2.3. Plastid Transformation and Recovery of Transplastomic Plants
2.4. Tracking Transgenic Plastids Using Green Fluorescence Protein (GFP)
2.5. Tracking Transgene Integration through PCR Approach
3. Discussion
4. Materials and Methods
4.1. Choice of Explant Material and Callus Induction
4.2. Shoot Induction, Multiplication, and Rooting
4.3. Construction of Species-Specific Chloroplast Transformation Vectors
4.4. Sugarcane Plastome Transformation
4.5. Tracking Fluorescent Protein (gfp) in Plastids/Cells
4.6. Total Cellular DNA Extraction and PCR Analyses
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mustafa, G.; Khan, M.S. Transmission of Engineered Plastids in Sugarcane, a C4 Monocotyledonous Plant, Reveals that Sorting of Preprogrammed Progenitor Cells Produce Heteroplasmy. Plants 2021, 10, 26. https://doi.org/10.3390/plants10010026
Mustafa G, Khan MS. Transmission of Engineered Plastids in Sugarcane, a C4 Monocotyledonous Plant, Reveals that Sorting of Preprogrammed Progenitor Cells Produce Heteroplasmy. Plants. 2021; 10(1):26. https://doi.org/10.3390/plants10010026
Chicago/Turabian StyleMustafa, Ghulam, and Muhammad Sarwar Khan. 2021. "Transmission of Engineered Plastids in Sugarcane, a C4 Monocotyledonous Plant, Reveals that Sorting of Preprogrammed Progenitor Cells Produce Heteroplasmy" Plants 10, no. 1: 26. https://doi.org/10.3390/plants10010026