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Article

Phytochemical Screening of Himatanthus sucuuba (Spruce) Woodson (Apocynaceae) Latex, In Vitro Cytotoxicity and Incision Wound Repair in Mice

by
Oscar Herrera-Calderón
1,*,
Lisbeth Lucia Calero-Armijos
1,
Wilson Cardona-G
2,
Angie Herrera-R
2,
Gustavo Moreno
2,
Majed A. Algarni
3,
Mohammed Alqarni
4 and
Gaber El-Saber Batiha
5
1
Department of Pharmacology, Bromatology, Toxicology, Faculty of Pharmacy and Biochemistry, Universidad Nacional Mayor de San Marcos, Jr. Puno 1002, Lima 15001, Peru
2
Química de Plantas Colombianas, Faculty of Exact and Natural Sciences, Institute of Chemistry, University of Antioquia (UdeA), Calle 70 No. 52–21, Medellín 1226, Colombia
3
Department of Clinical Pharmacy, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
4
Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
5
Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22515, Egypt
*
Author to whom correspondence should be addressed.
Plants 2021, 10(10), 2197; https://doi.org/10.3390/plants10102197
Submission received: 27 September 2021 / Revised: 12 October 2021 / Accepted: 13 October 2021 / Published: 16 October 2021
(This article belongs to the Special Issue Medicinal Plant Extracts)
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Abstract

Himatanthus sucuuba, also known as “Bellaco caspi”, is a medicinal plant whose latex, stem bark, and leaves possess phenolic acids, lupeol, β-dihydro-plumbericinic acid, plumericin, and plumeride, among other components. Some of these have been linked to such biological activities as antiulcer, anti-inflammatory, and wound healing. The aim of this study was to determine the phytochemical compounds of H. sucuuba latex, as well as its in vitro cytotoxicity and wound healing effect in mice. Latex was collected in the province of Iquitos, Peru. Phytochemical analysis was carried out with UPLC-ESI-MS/MS. The cytotoxicity was evaluated on two colon tumor cell lines (SW480 and SW620) and non-malignant cells (human keratinocytes, HaCaT, and Chinese hamster ovary, CHO-K1). The mice were distributed into two groups, as follows: Group I—control (n = 10; without treatment); II—(n = 10) H. sucuuba latex; wounds were induced with a scalpel in the dorsal–cervical area and treatments were applied topically twice a day on the incision for 10 days. Molecular docking was carried out on the glycogen synthase kinase 3β protein. Twenty-four chemical compounds were determined, mainly flavonoid-type compounds. Latex did not have a cytotoxic effect on tumor cells with IC50 values of more than 500 µg/mL. The latex had a regenerative effect on wounds in mice. Acacetin-7-O-neohesperidoside had the best docking score of −9.9 kcal/mol. In conclusion, H. sucuuba latex had a wound healing effect in mice, as confirmed by histological study. However, a non-cytotoxic effect was observed on colon tumor cells SW480 and SW620.
Keywords: latex; wound healing; anticancer; colon cancer; cytotoxicity; docking molecular latex; wound healing; anticancer; colon cancer; cytotoxicity; docking molecular

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MDPI and ACS Style

Herrera-Calderón, O.; Calero-Armijos, L.L.; Cardona-G, W.; Herrera-R, A.; Moreno, G.; Algarni, M.A.; Alqarni, M.; El-Saber Batiha, G. Phytochemical Screening of Himatanthus sucuuba (Spruce) Woodson (Apocynaceae) Latex, In Vitro Cytotoxicity and Incision Wound Repair in Mice. Plants 2021, 10, 2197. https://doi.org/10.3390/plants10102197

AMA Style

Herrera-Calderón O, Calero-Armijos LL, Cardona-G W, Herrera-R A, Moreno G, Algarni MA, Alqarni M, El-Saber Batiha G. Phytochemical Screening of Himatanthus sucuuba (Spruce) Woodson (Apocynaceae) Latex, In Vitro Cytotoxicity and Incision Wound Repair in Mice. Plants. 2021; 10(10):2197. https://doi.org/10.3390/plants10102197

Chicago/Turabian Style

Herrera-Calderón, Oscar, Lisbeth Lucia Calero-Armijos, Wilson Cardona-G, Angie Herrera-R, Gustavo Moreno, Majed A. Algarni, Mohammed Alqarni, and Gaber El-Saber Batiha. 2021. "Phytochemical Screening of Himatanthus sucuuba (Spruce) Woodson (Apocynaceae) Latex, In Vitro Cytotoxicity and Incision Wound Repair in Mice" Plants 10, no. 10: 2197. https://doi.org/10.3390/plants10102197

APA Style

Herrera-Calderón, O., Calero-Armijos, L. L., Cardona-G, W., Herrera-R, A., Moreno, G., Algarni, M. A., Alqarni, M., & El-Saber Batiha, G. (2021). Phytochemical Screening of Himatanthus sucuuba (Spruce) Woodson (Apocynaceae) Latex, In Vitro Cytotoxicity and Incision Wound Repair in Mice. Plants, 10(10), 2197. https://doi.org/10.3390/plants10102197

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