Exogenous Application of Alpha-Lipoic Acid Mitigates Salt-Induced Oxidative Damage in Sorghum Plants through Regulation Growth, Leaf Pigments, Ionic Homeostasis, Antioxidant Enzymes, and Expression of Salt Stress Responsive Genes
Abstract
:1. Introduction
2. Materials and Method
2.1. Growth Conditions and Experimental Design
2.2. Determination of Growth Parameters
2.3. Membrane Stability Index (MSI), Hydrogen Peroxide and Lipid Peroxidation
2.4. Determination of Leaf Pigments
2.5. Assay of Antioxidant Enzymes
2.6. Gene Expression
2.7. Statistics
3. Results
3.1. Effect of ALA on Growth Parameters
3.2. Effect of ALA on the Membranes’ Stability and Leaf Oxidative Damage
3.3. Effect of ALA on the Photosynthetic Pigments
3.4. Effect of ALA on the Activities of Antioxidant Enzymes
3.5. Effect of ALA on Na, K, Ca and Na/K Ratio
3.6. Effect of ALA on the Expression of SOS1, NHX1 and HKT1
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Sequence | NCBI Accession | |
---|---|---|---|
SOS1 | F | 5′-ACTTGCAGGAGGAATACAAC-3′ | NM001176582 |
R | 5′- CGAGAAGAGAAGACCACATC-3′, | ||
HKT1 | F | 5′-TGCTAATGTTTATCGTGCTG-3′ | HQ845286 |
R | 5′-AGGCTGATCCTCTTCCTAAC-3′ | ||
NHX1 | F | 5′-CGTGATGTCGCATTACACCT-3′ | AY270040 |
R | 5′- CTGGCAAACTCCCACTTCTC-3′ | ||
GAPDH | F | 5′-TGACGACATCAAGAAGGTGGTG-3′ | NM_001082253 |
R | 5′-:GAAGGTGGAGGAGTGGGTGTC-3′ |
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Youssef, M.H.M.; Raafat, A.; El-Yazied, A.A.; Selim, S.; Azab, E.; Khojah, E.; El Nahhas, N.; Ibrahim, M.F.M. Exogenous Application of Alpha-Lipoic Acid Mitigates Salt-Induced Oxidative Damage in Sorghum Plants through Regulation Growth, Leaf Pigments, Ionic Homeostasis, Antioxidant Enzymes, and Expression of Salt Stress Responsive Genes. Plants 2021, 10, 2519. https://doi.org/10.3390/plants10112519
Youssef MHM, Raafat A, El-Yazied AA, Selim S, Azab E, Khojah E, El Nahhas N, Ibrahim MFM. Exogenous Application of Alpha-Lipoic Acid Mitigates Salt-Induced Oxidative Damage in Sorghum Plants through Regulation Growth, Leaf Pigments, Ionic Homeostasis, Antioxidant Enzymes, and Expression of Salt Stress Responsive Genes. Plants. 2021; 10(11):2519. https://doi.org/10.3390/plants10112519
Chicago/Turabian StyleYoussef, Montaser H. M., Aly Raafat, Ahmed Abou El-Yazied, Samy Selim, Ehab Azab, Ebtihal Khojah, Nihal El Nahhas, and Mohamed F. M. Ibrahim. 2021. "Exogenous Application of Alpha-Lipoic Acid Mitigates Salt-Induced Oxidative Damage in Sorghum Plants through Regulation Growth, Leaf Pigments, Ionic Homeostasis, Antioxidant Enzymes, and Expression of Salt Stress Responsive Genes" Plants 10, no. 11: 2519. https://doi.org/10.3390/plants10112519